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Transforming growth factor-beta1 downregulation of Smad1 gene expression in rat hepatic stellate cells.
Am J Physiol Gastrointest Liver Physiol. 2003 Sep; 285(3):G539-46.AJ

Abstract

Smads are intracellular signaling molecules of the transforming growth factor-beta (TGF-beta) superfamily that play an important role in the activation of hepatic stellate cells (HSCs) and hepatic fibrosis. Excepting the regulation of Smad7, receptor-regulated Smad gene expression is still unclear. We employed rat HSCs to investigate the expression and regulation of the Smad1 gene, which is a bone morphogenetic protein (BMP) receptor-regulated Smad. We found that the expression and phosphorylation of Smad1 are increased during the activation of HSCs. Moreover, TGF-beta significantly inhibits Smad1 gene expression in HSCs in a time- and dose-dependent manner. Furthermore, although both TGF-beta1 and BMP2 stimulate the activation of HSCs, they have different effects on HSC proliferation. In conclusion, Smad1 expression and phosphorylation are increased during the activation of HSCs and TGF-beta1 significantly inhibits the expression of the Smad1 gene.

Authors+Show Affiliations

Faculty of Pharmacy, University of Manitoba, Winnipeg, MB R3T 2N2 Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12791600

Citation

Shen, Hong, et al. "Transforming Growth Factor-beta1 Downregulation of Smad1 Gene Expression in Rat Hepatic Stellate Cells." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 285, no. 3, 2003, pp. G539-46.
Shen H, Huang G, Hadi M, et al. Transforming growth factor-beta1 downregulation of Smad1 gene expression in rat hepatic stellate cells. Am J Physiol Gastrointest Liver Physiol. 2003;285(3):G539-46.
Shen, H., Huang, G., Hadi, M., Choy, P., Zhang, M., Minuk, G. Y., Chen, Y., & Gong, Y. (2003). Transforming growth factor-beta1 downregulation of Smad1 gene expression in rat hepatic stellate cells. American Journal of Physiology. Gastrointestinal and Liver Physiology, 285(3), G539-46.
Shen H, et al. Transforming Growth Factor-beta1 Downregulation of Smad1 Gene Expression in Rat Hepatic Stellate Cells. Am J Physiol Gastrointest Liver Physiol. 2003;285(3):G539-46. PubMed PMID: 12791600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transforming growth factor-beta1 downregulation of Smad1 gene expression in rat hepatic stellate cells. AU - Shen,Hong, AU - Huang,Guojiang, AU - Hadi,Mohammed, AU - Choy,Patrick, AU - Zhang,Manna, AU - Minuk,Gerald Y, AU - Chen,Yongping, AU - Gong,Yuewen, Y1 - 2003/06/04/ PY - 2003/6/7/pubmed PY - 2003/9/27/medline PY - 2003/6/7/entrez SP - G539 EP - 46 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 285 IS - 3 N2 - Smads are intracellular signaling molecules of the transforming growth factor-beta (TGF-beta) superfamily that play an important role in the activation of hepatic stellate cells (HSCs) and hepatic fibrosis. Excepting the regulation of Smad7, receptor-regulated Smad gene expression is still unclear. We employed rat HSCs to investigate the expression and regulation of the Smad1 gene, which is a bone morphogenetic protein (BMP) receptor-regulated Smad. We found that the expression and phosphorylation of Smad1 are increased during the activation of HSCs. Moreover, TGF-beta significantly inhibits Smad1 gene expression in HSCs in a time- and dose-dependent manner. Furthermore, although both TGF-beta1 and BMP2 stimulate the activation of HSCs, they have different effects on HSC proliferation. In conclusion, Smad1 expression and phosphorylation are increased during the activation of HSCs and TGF-beta1 significantly inhibits the expression of the Smad1 gene. SN - 0193-1857 UR - https://www.unboundmedicine.com/medline/citation/12791600/Transforming_growth_factor_beta1_downregulation_of_Smad1_gene_expression_in_rat_hepatic_stellate_cells_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.00436.2002?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -