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Sphingosine 1-phosphate in amniotic fluid modulates cyclooxygenase-2 expression in human amnion-derived WISH cells.
J Biol Chem. 2003 Aug 22; 278(34):31731-6.JB

Abstract

The metabolism of arachidonic acid, in particular the generation of prostaglandins (PGs), has been proposed to play a key role in the regulation of labor. Moreover, several extracellular proteins have been reported to modulate PG synthesis in amnion cells. In this study, we found that lipid components dissolved in the amniotic fluid modulate PG synthesis in WISH human amnion cells and identified one of these components as a sphingosine 1-phosphate (S1P). WISH cells express several S1P receptors including S1P1, S1P2, and S1P3. When WISH cells were stimulated with S1P, PGE2 synthesis increased in a concentration-dependent manner, showing maximal activity at around 100 nM. S1P treatment also caused the up-regulation of cyclooxygenase-2 (COX-2) mRNA and protein, which was apparent within 3-12 h of stimulation. In terms of the intracellular signaling pathway of S1P-induced WISH cell activation, we found that S1P stimulated two kinds of MAPK, ERK, and p38 kinase. We examined the roles of these two MAPKs in S1P-induced COX-2 expression. S1P-induced COX-2 expression was blocked completely by PD-98059 but not by SB-203580, suggesting that ERK has a critical role in the process. Transfection of S1P1 or S1P3 but not of S1P2 antisense oligonucleotide inhibited S1P-induced COX-2 expression and PGE2 production in WISH cells, indicating the involvements of S1P1 and S1P3 in the processes. This study demonstrates the physiological role of S1P in amniotic fluid and its effect on the modulation of COX-2 expression and PGs synthesis in WISH cells.

Authors+Show Affiliations

Medical Research Center for Cancer Molecular Therapy and Department of Biochemistry, College of Medicine, Dong-A University, Busan 602-714, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12796504

Citation

Kim, Jung Im, et al. "Sphingosine 1-phosphate in Amniotic Fluid Modulates Cyclooxygenase-2 Expression in Human Amnion-derived WISH Cells." The Journal of Biological Chemistry, vol. 278, no. 34, 2003, pp. 31731-6.
Kim JI, Jo EJ, Lee HY, et al. Sphingosine 1-phosphate in amniotic fluid modulates cyclooxygenase-2 expression in human amnion-derived WISH cells. J Biol Chem. 2003;278(34):31731-6.
Kim, J. I., Jo, E. J., Lee, H. Y., Cha, M. S., Min, J. K., Choi, C. H., Lee, Y. M., Choi, Y. A., Baek, S. H., Ryu, S. H., Lee, K. S., Kwak, J. Y., & Bae, Y. S. (2003). Sphingosine 1-phosphate in amniotic fluid modulates cyclooxygenase-2 expression in human amnion-derived WISH cells. The Journal of Biological Chemistry, 278(34), 31731-6.
Kim JI, et al. Sphingosine 1-phosphate in Amniotic Fluid Modulates Cyclooxygenase-2 Expression in Human Amnion-derived WISH Cells. J Biol Chem. 2003 Aug 22;278(34):31731-6. PubMed PMID: 12796504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sphingosine 1-phosphate in amniotic fluid modulates cyclooxygenase-2 expression in human amnion-derived WISH cells. AU - Kim,Jung Im, AU - Jo,Eun Jin, AU - Lee,Ha-Young, AU - Cha,Moon Seok, AU - Min,Jung Kee, AU - Choi,Chang Hwan, AU - Lee,Yong Moon, AU - Choi,Young-Ae, AU - Baek,Suk-Hwan, AU - Ryu,Sung Ho, AU - Lee,Kyu Sup, AU - Kwak,Jong-Young, AU - Bae,Yoe-Sik, Y1 - 2003/06/09/ PY - 2003/6/11/pubmed PY - 2003/10/30/medline PY - 2003/6/11/entrez SP - 31731 EP - 6 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 278 IS - 34 N2 - The metabolism of arachidonic acid, in particular the generation of prostaglandins (PGs), has been proposed to play a key role in the regulation of labor. Moreover, several extracellular proteins have been reported to modulate PG synthesis in amnion cells. In this study, we found that lipid components dissolved in the amniotic fluid modulate PG synthesis in WISH human amnion cells and identified one of these components as a sphingosine 1-phosphate (S1P). WISH cells express several S1P receptors including S1P1, S1P2, and S1P3. When WISH cells were stimulated with S1P, PGE2 synthesis increased in a concentration-dependent manner, showing maximal activity at around 100 nM. S1P treatment also caused the up-regulation of cyclooxygenase-2 (COX-2) mRNA and protein, which was apparent within 3-12 h of stimulation. In terms of the intracellular signaling pathway of S1P-induced WISH cell activation, we found that S1P stimulated two kinds of MAPK, ERK, and p38 kinase. We examined the roles of these two MAPKs in S1P-induced COX-2 expression. S1P-induced COX-2 expression was blocked completely by PD-98059 but not by SB-203580, suggesting that ERK has a critical role in the process. Transfection of S1P1 or S1P3 but not of S1P2 antisense oligonucleotide inhibited S1P-induced COX-2 expression and PGE2 production in WISH cells, indicating the involvements of S1P1 and S1P3 in the processes. This study demonstrates the physiological role of S1P in amniotic fluid and its effect on the modulation of COX-2 expression and PGs synthesis in WISH cells. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/12796504/Sphingosine_1_phosphate_in_amniotic_fluid_modulates_cyclooxygenase_2_expression_in_human_amnion_derived_WISH_cells_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=12796504 DB - PRIME DP - Unbound Medicine ER -