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Simultaneous determination of bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 05; 791(1-2):171-7.JC

Abstract

We devised a sensitive and simple method to simultaneously determine bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. Bromvalerylurea and allylisopropylacetylurea were extracted using an Extrelut column with an internal standard, 2-bromohexanoylurea, followed by derivatization with heptafluorobutyric anhydride. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of both compounds were linear in the concentration range from 0.01 to 10 microg/ml in both blood and urine samples. The lower limits of detection of bromvalerylurea and allylisopropylacetylurea were 0.005 and 0.005 microg/ml, respectively. This method proved most useful in accurately identifying these drugs in blood and urine from an autopsied individual.

Authors+Show Affiliations

Department of Forensic Pathology and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12798177

Citation

Kudo, Keiko, et al. "Simultaneous Determination of Bromvalerylurea and Allylisopropylacetylurea in Human Blood and Urine By Gas Chromatography-mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 791, no. 1-2, 2003, pp. 171-7.
Kudo K, Kiyoshima A, Ohtsuka Y, et al. Simultaneous determination of bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2003;791(1-2):171-7.
Kudo, K., Kiyoshima, A., Ohtsuka, Y., & Ikeda, N. (2003). Simultaneous determination of bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 791(1-2), 171-7.
Kudo K, et al. Simultaneous Determination of Bromvalerylurea and Allylisopropylacetylurea in Human Blood and Urine By Gas Chromatography-mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 5;791(1-2):171-7. PubMed PMID: 12798177.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. AU - Kudo,Keiko, AU - Kiyoshima,Akiko, AU - Ohtsuka,Yukio, AU - Ikeda,Noriaki, PY - 2003/6/12/pubmed PY - 2004/3/5/medline PY - 2003/6/12/entrez SP - 171 EP - 7 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 791 IS - 1-2 N2 - We devised a sensitive and simple method to simultaneously determine bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography-mass spectrometry. Bromvalerylurea and allylisopropylacetylurea were extracted using an Extrelut column with an internal standard, 2-bromohexanoylurea, followed by derivatization with heptafluorobutyric anhydride. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of both compounds were linear in the concentration range from 0.01 to 10 microg/ml in both blood and urine samples. The lower limits of detection of bromvalerylurea and allylisopropylacetylurea were 0.005 and 0.005 microg/ml, respectively. This method proved most useful in accurately identifying these drugs in blood and urine from an autopsied individual. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/12798177/Simultaneous_determination_of_bromvalerylurea_and_allylisopropylacetylurea_in_human_blood_and_urine_by_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570023203002198 DB - PRIME DP - Unbound Medicine ER -