Tags

Type your tag names separated by a space and hit enter

Estrogen receptor expression in lumbosacral dorsal root ganglion cells innervating the female rat urinary bladder.
Auton Neurosci. 2003 May 30; 105(2):90-100.AN

Abstract

We have investigated whether bladder afferent neurons are likely to be targets for circulating estrogens by mapping estrogen receptor (ER) distribution in lumbosacral dorsal root ganglia (DRG) of adult female rats. Sensory neurons innervating either the detrusor or trigone regions were identified by application of fluorescent retrograde tracer dyes to the bladder wall. Labelled neurons were classified by their immunoreactivity for either type of ER (ERalpha or ERbeta) and further compared with subpopulations of neurons containing substance P, calcitonin gene-related peptide and vanilloid receptor (a marker of polymodal nociceptors). Both ER types were expressed in numerous sensory neurons of either upper lumbar (L1/L2) or lower lumbar/sacral (L6/S1) ganglia and there was almost complete coexpression of ERalpha and ERbeta. ER-positive neurons were mainly small-medium size (18-25-microm diameter), indicating that they may be nociceptors and/or supply visceral targets. Most bladder-projecting neurons expressed ERs and the majority of these also expressed neuropeptides or vanilloid receptor. Afferent neurons supplying detrusor and trigone regions had similar immunohistochemical features. About a third of the bladder-projecting neurons expressed both ER and vanilloid receptor, suggesting a mechanism by which estrogens could influence bladder pain. The prevalence of different chemical classes of ER-positive bladder-projecting neurons was reflected throughout the entire population of neurons in dorsal root ganglia of these spinal levels, suggesting that neurons supplying other pelvic visceral targets may have similar chemical profiles. These results suggest that many functional classes of sensory neurons innervating the lower urinary tract are likely to be targets for circulating estrogens, including many nociceptor neurons. The coexistence of ERalpha and ERbeta suggests a broad range of potential mechanisms by which estrogens may exert their genomic effects in this system.

Authors+Show Affiliations

Prince of Wales Medical Research Institute, University of New South Wales, NSW 2052, Sydney, Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12798205

Citation

Bennett, Haley L., et al. "Estrogen Receptor Expression in Lumbosacral Dorsal Root Ganglion Cells Innervating the Female Rat Urinary Bladder." Autonomic Neuroscience : Basic & Clinical, vol. 105, no. 2, 2003, pp. 90-100.
Bennett HL, Gustafsson JA, Keast JR. Estrogen receptor expression in lumbosacral dorsal root ganglion cells innervating the female rat urinary bladder. Auton Neurosci. 2003;105(2):90-100.
Bennett, H. L., Gustafsson, J. A., & Keast, J. R. (2003). Estrogen receptor expression in lumbosacral dorsal root ganglion cells innervating the female rat urinary bladder. Autonomic Neuroscience : Basic & Clinical, 105(2), 90-100.
Bennett HL, Gustafsson JA, Keast JR. Estrogen Receptor Expression in Lumbosacral Dorsal Root Ganglion Cells Innervating the Female Rat Urinary Bladder. Auton Neurosci. 2003 May 30;105(2):90-100. PubMed PMID: 12798205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Estrogen receptor expression in lumbosacral dorsal root ganglion cells innervating the female rat urinary bladder. AU - Bennett,Haley L, AU - Gustafsson,Jan Ake, AU - Keast,Janet R, PY - 2003/6/12/pubmed PY - 2003/8/2/medline PY - 2003/6/12/entrez SP - 90 EP - 100 JF - Autonomic neuroscience : basic & clinical JO - Auton Neurosci VL - 105 IS - 2 N2 - We have investigated whether bladder afferent neurons are likely to be targets for circulating estrogens by mapping estrogen receptor (ER) distribution in lumbosacral dorsal root ganglia (DRG) of adult female rats. Sensory neurons innervating either the detrusor or trigone regions were identified by application of fluorescent retrograde tracer dyes to the bladder wall. Labelled neurons were classified by their immunoreactivity for either type of ER (ERalpha or ERbeta) and further compared with subpopulations of neurons containing substance P, calcitonin gene-related peptide and vanilloid receptor (a marker of polymodal nociceptors). Both ER types were expressed in numerous sensory neurons of either upper lumbar (L1/L2) or lower lumbar/sacral (L6/S1) ganglia and there was almost complete coexpression of ERalpha and ERbeta. ER-positive neurons were mainly small-medium size (18-25-microm diameter), indicating that they may be nociceptors and/or supply visceral targets. Most bladder-projecting neurons expressed ERs and the majority of these also expressed neuropeptides or vanilloid receptor. Afferent neurons supplying detrusor and trigone regions had similar immunohistochemical features. About a third of the bladder-projecting neurons expressed both ER and vanilloid receptor, suggesting a mechanism by which estrogens could influence bladder pain. The prevalence of different chemical classes of ER-positive bladder-projecting neurons was reflected throughout the entire population of neurons in dorsal root ganglia of these spinal levels, suggesting that neurons supplying other pelvic visceral targets may have similar chemical profiles. These results suggest that many functional classes of sensory neurons innervating the lower urinary tract are likely to be targets for circulating estrogens, including many nociceptor neurons. The coexistence of ERalpha and ERbeta suggests a broad range of potential mechanisms by which estrogens may exert their genomic effects in this system. SN - 1566-0702 UR - https://www.unboundmedicine.com/medline/citation/12798205/Estrogen_receptor_expression_in_lumbosacral_dorsal_root_ganglion_cells_innervating_the_female_rat_urinary_bladder_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1566-0702(03)00044-4 DB - PRIME DP - Unbound Medicine ER -