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Expression and regulation of scavenger receptor class B type I (SR-BI) in gall bladder epithelium.
Gut. 2003 Jul; 52(7):1017-24.Gut

Abstract

BACKGROUND AND AIMS

Biliary lipid absorption by the gall bladder mucosa and the cholesterol content of the gall bladder wall appear to play a role in cholesterol gall stone formation. As the scavenger receptor class B type I (SR- BI) regulates cellular cholesterol uptake, we studied its expression in human and murine gall bladders, its regulation by increased biliary lipid content, and its role in gall stone formation.

METHODS AND RESULTS

Using immunohistochemistry, SR-BI was found in the apical domain of human gall bladder epithelial cells. Immunoblotting of isolated membranes from gall bladder epithelial cells showed a specific signal for the 82 kDa SR-BI protein. In C57BL/6 mice, SR-BI was also found in the gall bladder epithelium. Using western blot analysis, an inverse relationship was observed between biliary cholesterol concentration and SR-BI expression in murine gall bladder mucosa. By comparing lithogenic diet fed wild-type and SR-BI deficient mice, gall bladder wall cholesterol content and gall stone formation were not found to be dependent on SR-BI expression.

CONCLUSIONS

(i) SR-BI is expressed in both human and murine gall bladder epithelium; (ii) biliary cholesterol hypersecretion is associated with decreased gall bladder SR-BI expression in mice; and (iii) murine SR-BI is not essential in controlling gall bladder wall cholesterol content and gall stone formation during diet induced cholelithiasis.

Authors+Show Affiliations

Departamento de Gastroenterología, Facultad de Medicina, Pontificia Universidad Católica, Marcoleta 367, Santiago, Chile. jfmiquel@med.puc.clNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12801960

Citation

Miquel, J F., et al. "Expression and Regulation of Scavenger Receptor Class B Type I (SR-BI) in Gall Bladder Epithelium." Gut, vol. 52, no. 7, 2003, pp. 1017-24.
Miquel JF, Moreno M, Amigo L, et al. Expression and regulation of scavenger receptor class B type I (SR-BI) in gall bladder epithelium. Gut. 2003;52(7):1017-24.
Miquel, J. F., Moreno, M., Amigo, L., Molina, H., Mardones, P., Wistuba, I. I., & Rigotti, A. (2003). Expression and regulation of scavenger receptor class B type I (SR-BI) in gall bladder epithelium. Gut, 52(7), 1017-24.
Miquel JF, et al. Expression and Regulation of Scavenger Receptor Class B Type I (SR-BI) in Gall Bladder Epithelium. Gut. 2003;52(7):1017-24. PubMed PMID: 12801960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression and regulation of scavenger receptor class B type I (SR-BI) in gall bladder epithelium. AU - Miquel,J F, AU - Moreno,M, AU - Amigo,L, AU - Molina,H, AU - Mardones,P, AU - Wistuba,I I, AU - Rigotti,A, PY - 2003/6/13/pubmed PY - 2003/8/20/medline PY - 2003/6/13/entrez SP - 1017 EP - 24 JF - Gut JO - Gut VL - 52 IS - 7 N2 - BACKGROUND AND AIMS: Biliary lipid absorption by the gall bladder mucosa and the cholesterol content of the gall bladder wall appear to play a role in cholesterol gall stone formation. As the scavenger receptor class B type I (SR- BI) regulates cellular cholesterol uptake, we studied its expression in human and murine gall bladders, its regulation by increased biliary lipid content, and its role in gall stone formation. METHODS AND RESULTS: Using immunohistochemistry, SR-BI was found in the apical domain of human gall bladder epithelial cells. Immunoblotting of isolated membranes from gall bladder epithelial cells showed a specific signal for the 82 kDa SR-BI protein. In C57BL/6 mice, SR-BI was also found in the gall bladder epithelium. Using western blot analysis, an inverse relationship was observed between biliary cholesterol concentration and SR-BI expression in murine gall bladder mucosa. By comparing lithogenic diet fed wild-type and SR-BI deficient mice, gall bladder wall cholesterol content and gall stone formation were not found to be dependent on SR-BI expression. CONCLUSIONS: (i) SR-BI is expressed in both human and murine gall bladder epithelium; (ii) biliary cholesterol hypersecretion is associated with decreased gall bladder SR-BI expression in mice; and (iii) murine SR-BI is not essential in controlling gall bladder wall cholesterol content and gall stone formation during diet induced cholelithiasis. SN - 0017-5749 UR - https://www.unboundmedicine.com/medline/citation/12801960/Expression_and_regulation_of_scavenger_receptor_class_B_type_I__SR_BI__in_gall_bladder_epithelium_ L2 - http://gut.bmj.com/cgi/pmidlookup?view=long&pmid=12801960 DB - PRIME DP - Unbound Medicine ER -