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Antitumor activity of balsam fir oil: production of reactive oxygen species induced by alpha-humulene as possible mechanism of action.
Planta Med. 2003 May; 69(5):402-7.PM

Abstract

The antitumor activity of the essential oil of Abies balsamea (balsam fir oil) was evaluated against several solid tumor cell lines including MCF-7, PC-3, A-549, DLD-1, M4BEU and CT-26. Balsam fir oil was found to be active against all the solid tumor cell lines tested, with GI 50 values ranging between 0.76 and 1.7 mg/mL. The oil was analyzed by GC-MS and the cytotoxicity of each oil constituent was determined. Balsam fir oil is essentially constituted of monoterpenes tau; 96 %) and some sesquiterpenes. All the compounds tested were inactive (tau; 250 microM) except for alpha-humulene (GI50 = 55 to 73 microM) which thus seems responsible for the cytotoxicity of the oil. We also tested the cytotoxicity of caryophyllene oxide, which proved inactive, and gamma-caryophyllene which was found to be active against all solid tumor cell lines tested. We evaluated the effects of balsam fir oil and alpha-humulene on the cellular glutathione (GSH) content and on the production of reactive oxygen species (ROS). Balsam fir oil and alpha-humulene induced a dose- and time-dependent decrease in cellular GSH content and an increase in ROS production. These results suggest that GSH depletion and ROS production may be implicated in the cytotoxicity of alpha-humulene and balsam fir oil.

Authors+Show Affiliations

UMR-Institut National de la Santé et de la Recherche Médicale, Clermont-Ferrand, France. jean_legault@uqac.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12802719

Citation

Legault, Jean, et al. "Antitumor Activity of Balsam Fir Oil: Production of Reactive Oxygen Species Induced By Alpha-humulene as Possible Mechanism of Action." Planta Medica, vol. 69, no. 5, 2003, pp. 402-7.
Legault J, Dahl W, Debiton E, et al. Antitumor activity of balsam fir oil: production of reactive oxygen species induced by alpha-humulene as possible mechanism of action. Planta Med. 2003;69(5):402-7.
Legault, J., Dahl, W., Debiton, E., Pichette, A., & Madelmont, J. C. (2003). Antitumor activity of balsam fir oil: production of reactive oxygen species induced by alpha-humulene as possible mechanism of action. Planta Medica, 69(5), 402-7.
Legault J, et al. Antitumor Activity of Balsam Fir Oil: Production of Reactive Oxygen Species Induced By Alpha-humulene as Possible Mechanism of Action. Planta Med. 2003;69(5):402-7. PubMed PMID: 12802719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antitumor activity of balsam fir oil: production of reactive oxygen species induced by alpha-humulene as possible mechanism of action. AU - Legault,Jean, AU - Dahl,Wivecke, AU - Debiton,Eric, AU - Pichette,André, AU - Madelmont,Jean-Claude, PY - 2003/6/13/pubmed PY - 2003/9/30/medline PY - 2003/6/13/entrez SP - 402 EP - 7 JF - Planta medica JO - Planta Med VL - 69 IS - 5 N2 - The antitumor activity of the essential oil of Abies balsamea (balsam fir oil) was evaluated against several solid tumor cell lines including MCF-7, PC-3, A-549, DLD-1, M4BEU and CT-26. Balsam fir oil was found to be active against all the solid tumor cell lines tested, with GI 50 values ranging between 0.76 and 1.7 mg/mL. The oil was analyzed by GC-MS and the cytotoxicity of each oil constituent was determined. Balsam fir oil is essentially constituted of monoterpenes tau; 96 %) and some sesquiterpenes. All the compounds tested were inactive (tau; 250 microM) except for alpha-humulene (GI50 = 55 to 73 microM) which thus seems responsible for the cytotoxicity of the oil. We also tested the cytotoxicity of caryophyllene oxide, which proved inactive, and gamma-caryophyllene which was found to be active against all solid tumor cell lines tested. We evaluated the effects of balsam fir oil and alpha-humulene on the cellular glutathione (GSH) content and on the production of reactive oxygen species (ROS). Balsam fir oil and alpha-humulene induced a dose- and time-dependent decrease in cellular GSH content and an increase in ROS production. These results suggest that GSH depletion and ROS production may be implicated in the cytotoxicity of alpha-humulene and balsam fir oil. SN - 0032-0943 UR - https://www.unboundmedicine.com/medline/citation/12802719/Antitumor_activity_of_balsam_fir_oil:_production_of_reactive_oxygen_species_induced_by_alpha_humulene_as_possible_mechanism_of_action_ DB - PRIME DP - Unbound Medicine ER -