Tags

Type your tag names separated by a space and hit enter

A genipin-crosslinked gelatin membrane as wound-dressing material: in vitro and in vivo studies.
J Biomater Sci Polym Ed 2003; 14(5):481-95JB

Abstract

A naturally occurring crosslinking agent (genipin) was used in this study to crosslink gelatin hydrogel to develop a wound-dressing membrane. The study was to investigate the in vitro characteristics of the genipin-crosslinked gelatin membrane. The glutaraldehyde-crosslinked counterpart, at a similar crosslinking degree, was used as control. Additionally, an in vivo experiment was undertaken to study the wound healings covered with the glutaraldehyde- and genipin-crosslinked dressings in a rat model. The in vitro results obtained suggested that crosslinking of gelatin membranes with glutaraldehyde or genipin may produce distinct crosslinking structures. The differences in crosslinking structure can significantly affect the mechanical property, water-vapor-transmission rate, swelling ratio, degradation against enzyme and cellular compatibility of the crosslinked membranes. In the in vivo study, it was found that the degree of inflammatory reaction for the wound treated with the genipin-crosslinked dressing was significantly less severe than that covered with the glutaraldehyde-crosslinked dressing throughout the entire course of the study. Additionally, the healing rate for the wound treated with the genipin-crosslinked dressing was notably faster than its glutaraldehyde-crosslinked counterpart.

Authors+Show Affiliations

Department of Chemical Engineering. National Tsing Hua University, Hsinchu, Taiwan 30013, ROC.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12807149

Citation

Chang, Wen-Hsiang, et al. "A Genipin-crosslinked Gelatin Membrane as Wound-dressing Material: in Vitro and in Vivo Studies." Journal of Biomaterials Science. Polymer Edition, vol. 14, no. 5, 2003, pp. 481-95.
Chang WH, Chang Y, Lai PH, et al. A genipin-crosslinked gelatin membrane as wound-dressing material: in vitro and in vivo studies. J Biomater Sci Polym Ed. 2003;14(5):481-95.
Chang, W. H., Chang, Y., Lai, P. H., & Sung, H. W. (2003). A genipin-crosslinked gelatin membrane as wound-dressing material: in vitro and in vivo studies. Journal of Biomaterials Science. Polymer Edition, 14(5), pp. 481-95.
Chang WH, et al. A Genipin-crosslinked Gelatin Membrane as Wound-dressing Material: in Vitro and in Vivo Studies. J Biomater Sci Polym Ed. 2003;14(5):481-95. PubMed PMID: 12807149.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A genipin-crosslinked gelatin membrane as wound-dressing material: in vitro and in vivo studies. AU - Chang,Wen-Hsiang, AU - Chang,Yen, AU - Lai,Po-Hong, AU - Sung,Hsing-Wen, PY - 2003/6/17/pubmed PY - 2003/9/18/medline PY - 2003/6/17/entrez SP - 481 EP - 95 JF - Journal of biomaterials science. Polymer edition JO - J Biomater Sci Polym Ed VL - 14 IS - 5 N2 - A naturally occurring crosslinking agent (genipin) was used in this study to crosslink gelatin hydrogel to develop a wound-dressing membrane. The study was to investigate the in vitro characteristics of the genipin-crosslinked gelatin membrane. The glutaraldehyde-crosslinked counterpart, at a similar crosslinking degree, was used as control. Additionally, an in vivo experiment was undertaken to study the wound healings covered with the glutaraldehyde- and genipin-crosslinked dressings in a rat model. The in vitro results obtained suggested that crosslinking of gelatin membranes with glutaraldehyde or genipin may produce distinct crosslinking structures. The differences in crosslinking structure can significantly affect the mechanical property, water-vapor-transmission rate, swelling ratio, degradation against enzyme and cellular compatibility of the crosslinked membranes. In the in vivo study, it was found that the degree of inflammatory reaction for the wound treated with the genipin-crosslinked dressing was significantly less severe than that covered with the glutaraldehyde-crosslinked dressing throughout the entire course of the study. Additionally, the healing rate for the wound treated with the genipin-crosslinked dressing was notably faster than its glutaraldehyde-crosslinked counterpart. SN - 0920-5063 UR - https://www.unboundmedicine.com/medline/citation/12807149/A_genipin_crosslinked_gelatin_membrane_as_wound_dressing_material:_in_vitro_and_in_vivo_studies_ L2 - http://www.tandfonline.com/doi/full/10.1163/156856203766652084 DB - PRIME DP - Unbound Medicine ER -