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Development and application of an alpha-face-specific radioimmunoassay for vitamin B12.
Biol Trace Elem Res. 1992 Nov; 35(2):153-66.BT

Abstract

The first development of an alpha-face-specific radioimmunoassay for vitamin B12 is described. Sheep, fed a cobalt-deficient diet, and immunized with a conjugate between Co-beta carboxypropyl cobalamin and keyhole limpet hemocyanin, were used to produce antisera. The antisera crossreacted with Co-beta derivatives of vitamin B12, but did not crossreact with the alpha-face vitamin B12 analog cobinamide. The antisera were used to develop a sensitive and reproducible radioimmunoassay that was free from contamination with the nonspecific vitamin B12 binding protein, R-protein. Both the radioimmunoassay and measurements of plasma concentrations of methylmalonic acid were applied to the diagnosis of cobalt/vitamin B12 deficiency in sheep. The assay correlated well with a commercially available radioassay and did not falsely detect normal vitamin B12 concentration in plasma samples containing elevated concentrations of methylmalonic acid.

Authors+Show Affiliations

Kennedy DG
Department of Agriculture for Northern Ireland, Stormont, Belfast, UK.
Blanchflower WJ
No affiliation info available
Young PB
No affiliation info available
Davidson WB
No affiliation info available

MeSH

AnimalsCobaltHemocyaninsImmune SeraImmunizationMiceRabbitsRadioimmunoassaySheepSheep DiseasesVitamin B 12Vitamin B 12 Deficiency

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1280981

Citation

Kennedy, D G., et al. "Development and Application of an Alpha-face-specific Radioimmunoassay for Vitamin B12." Biological Trace Element Research, vol. 35, no. 2, 1992, pp. 153-66.
Kennedy DG, Blanchflower WJ, Young PB, et al. Development and application of an alpha-face-specific radioimmunoassay for vitamin B12. Biol Trace Elem Res. 1992;35(2):153-66.
Kennedy, D. G., Blanchflower, W. J., Young, P. B., & Davidson, W. B. (1992). Development and application of an alpha-face-specific radioimmunoassay for vitamin B12. Biological Trace Element Research, 35(2), 153-66.
Kennedy DG, et al. Development and Application of an Alpha-face-specific Radioimmunoassay for Vitamin B12. Biol Trace Elem Res. 1992;35(2):153-66. PubMed PMID: 1280981.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and application of an alpha-face-specific radioimmunoassay for vitamin B12. AU - Kennedy,D G, AU - Blanchflower,W J, AU - Young,P B, AU - Davidson,W B, PY - 1992/11/1/pubmed PY - 1992/11/1/medline PY - 1992/11/1/entrez SP - 153 EP - 66 JF - Biological trace element research JO - Biol Trace Elem Res VL - 35 IS - 2 N2 - The first development of an alpha-face-specific radioimmunoassay for vitamin B12 is described. Sheep, fed a cobalt-deficient diet, and immunized with a conjugate between Co-beta carboxypropyl cobalamin and keyhole limpet hemocyanin, were used to produce antisera. The antisera crossreacted with Co-beta derivatives of vitamin B12, but did not crossreact with the alpha-face vitamin B12 analog cobinamide. The antisera were used to develop a sensitive and reproducible radioimmunoassay that was free from contamination with the nonspecific vitamin B12 binding protein, R-protein. Both the radioimmunoassay and measurements of plasma concentrations of methylmalonic acid were applied to the diagnosis of cobalt/vitamin B12 deficiency in sheep. The assay correlated well with a commercially available radioassay and did not falsely detect normal vitamin B12 concentration in plasma samples containing elevated concentrations of methylmalonic acid. SN - 0163-4984 UR - https://www.unboundmedicine.com/medline/citation/1280981/Development_and_application_of_an_alpha_face_specific_radioimmunoassay_for_vitamin_B12_ DB - PRIME DP - Unbound Medicine ER -