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Comparative study of allograft survival of heterotopic and orthotopic small bowel transplantation in rat.
Transplantation. 2003 Jun 15; 75(11):1895-7.T

Abstract

BACKGROUND

Small bowel allografts can either be placed heterotopically or orthotopically. In heterotopic small bowel transplantation (H-SBT), the host small intestine containing a substantial amount of gut-associated lymphoid tissue is removed, whereas in conventional orthotopic small bowel transplantation (O-SBT) it is retained. This study compared the allograft survival of H-SBT and O-SBT and evaluated the effect of retaining the host intestine in O-SBT in an altered O-SBT (AO-SBT) model.

METHODS

SBT was performed in a high-responder rat stain combination (blood group D Agouti --> Lewis). Immunosuppressive treatment consisting of a short course of FK506 (2 mg/kg/day IM for 3 days before transplantation and 0.3 mg/kg/day for 14 days after transplantation) was used.

RESULTS

Survival (mean +/- SD) of H-, O-, and AO-SBT untreated animals was 6.25+/-0.58 days, 6.5+/-0.58 days, and 6.7+/-0.25 days, respectively. With FK506 immunosuppression, survival of H-SBT animals was 49.3+/-13.17 days, whereas 75% (12/16) and 80% (4/5), respectively, of O-SBT and AO-SBT animals achieved indefinite survival (>120 days) with functioning grafts.

CONCLUSION

Our data suggest that heterotopic placement of intestinal allografts results in a more severe graft rejection than orthotopic placement. The indefinite survival of O-SBT is not due to of the removal of host intestine carrying a heavy load of gut-associated lymphoid tissue.

Authors+Show Affiliations

Division of Paediatric Surgery, Department of Surgery, University of Hong Kong Medical Centre, Queen Mary Hospital, Hong Kong SAR, P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12811251

Citation

Guo, Wei Hong, et al. "Comparative Study of Allograft Survival of Heterotopic and Orthotopic Small Bowel Transplantation in Rat." Transplantation, vol. 75, no. 11, 2003, pp. 1895-7.
Guo WH, Tian L, Dallmann MJ, et al. Comparative study of allograft survival of heterotopic and orthotopic small bowel transplantation in rat. Transplantation. 2003;75(11):1895-7.
Guo, W. H., Tian, L., Dallmann, M. J., & Tam, P. K. (2003). Comparative study of allograft survival of heterotopic and orthotopic small bowel transplantation in rat. Transplantation, 75(11), 1895-7.
Guo WH, et al. Comparative Study of Allograft Survival of Heterotopic and Orthotopic Small Bowel Transplantation in Rat. Transplantation. 2003 Jun 15;75(11):1895-7. PubMed PMID: 12811251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative study of allograft survival of heterotopic and orthotopic small bowel transplantation in rat. AU - Guo,Wei Hong, AU - Tian,Lina, AU - Dallmann,Margaret J, AU - Tam,Paul K H, PY - 2003/6/18/pubmed PY - 2003/7/16/medline PY - 2003/6/18/entrez SP - 1895 EP - 7 JF - Transplantation JO - Transplantation VL - 75 IS - 11 N2 - BACKGROUND: Small bowel allografts can either be placed heterotopically or orthotopically. In heterotopic small bowel transplantation (H-SBT), the host small intestine containing a substantial amount of gut-associated lymphoid tissue is removed, whereas in conventional orthotopic small bowel transplantation (O-SBT) it is retained. This study compared the allograft survival of H-SBT and O-SBT and evaluated the effect of retaining the host intestine in O-SBT in an altered O-SBT (AO-SBT) model. METHODS: SBT was performed in a high-responder rat stain combination (blood group D Agouti --> Lewis). Immunosuppressive treatment consisting of a short course of FK506 (2 mg/kg/day IM for 3 days before transplantation and 0.3 mg/kg/day for 14 days after transplantation) was used. RESULTS: Survival (mean +/- SD) of H-, O-, and AO-SBT untreated animals was 6.25+/-0.58 days, 6.5+/-0.58 days, and 6.7+/-0.25 days, respectively. With FK506 immunosuppression, survival of H-SBT animals was 49.3+/-13.17 days, whereas 75% (12/16) and 80% (4/5), respectively, of O-SBT and AO-SBT animals achieved indefinite survival (>120 days) with functioning grafts. CONCLUSION: Our data suggest that heterotopic placement of intestinal allografts results in a more severe graft rejection than orthotopic placement. The indefinite survival of O-SBT is not due to of the removal of host intestine carrying a heavy load of gut-associated lymphoid tissue. SN - 0041-1337 UR - https://www.unboundmedicine.com/medline/citation/12811251/Comparative_study_of_allograft_survival_of_heterotopic_and_orthotopic_small_bowel_transplantation_in_rat_ L2 - https://doi.org/10.1097/01.TP.0000065294.04793.11 DB - PRIME DP - Unbound Medicine ER -