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[Development and application of a genome specific PCR marker for Haynaldia villosa].
Yi Chuan Xue Bao. 2003 Apr; 30(4):350-6.YC

Abstract

Random amplified polymorphic DNA (RAPD) analysis was performed on common wheat Chinese Spring, H. villosa, addition lines of H. villosa chromosome in CS, substitution line 3V of H. villosa chromosome in Triticum aestivum. A genome specific polymorphic DNA segment from H. villosa, OPF02757, was obtained. On the basis of cloning and sequencing of OPF02757, two PCR primers were designed and a genome specific PCR marker for H. villosa was established. The PCR marker including 677 bp was localized on all the seven pairs of H. villosa chromosomes. The result of PCR amplification by the primers indicated that there was a specific band of 677 bp in the materials containing H. villosa Chromosome such as T. aestivum-H. villosa addition, T. aestivum-H. villosa substitution, T. aestivum-H. villosa amphidiploid, T. durum-H. villosa amphidiploid and H. villosum from different accessions, and there was no specific band of 677 bp if the materials did not contain H. villosa chromosome, such as T. aestivum, T. durum, Secale cereale, Hordeum vulgare, Thinopyrum elongatum, Thinopyrum intermedium. Therefore, the PCR maker of 677 bp is specific to H. villosa genome, and could be used as molecular marker for detection of chromosomes of H. villosa in wheat.

Authors+Show Affiliations

College of Crop Science, China Agricultural University, Beijing 100094, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12812061

Citation

Liu, Shou-Bin, et al. "[Development and Application of a Genome Specific PCR Marker for Haynaldia Villosa]." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 30, no. 4, 2003, pp. 350-6.
Liu SB, Tang ZH, You MS, et al. [Development and application of a genome specific PCR marker for Haynaldia villosa]. Yi Chuan Xue Bao. 2003;30(4):350-6.
Liu, S. B., Tang, Z. H., You, M. S., Li, B. Y., Song, J. M., & Liu, G. T. (2003). [Development and application of a genome specific PCR marker for Haynaldia villosa]. Yi Chuan Xue Bao = Acta Genetica Sinica, 30(4), 350-6.
Liu SB, et al. [Development and Application of a Genome Specific PCR Marker for Haynaldia Villosa]. Yi Chuan Xue Bao. 2003;30(4):350-6. PubMed PMID: 12812061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Development and application of a genome specific PCR marker for Haynaldia villosa]. AU - Liu,Shou-Bin, AU - Tang,Zhao-Hui, AU - You,Ming-Shan, AU - Li,Bao-Yun, AU - Song,Jian-Min, AU - Liu,Guang-Tian, PY - 2003/6/19/pubmed PY - 2003/12/13/medline PY - 2003/6/19/entrez SP - 350 EP - 6 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 30 IS - 4 N2 - Random amplified polymorphic DNA (RAPD) analysis was performed on common wheat Chinese Spring, H. villosa, addition lines of H. villosa chromosome in CS, substitution line 3V of H. villosa chromosome in Triticum aestivum. A genome specific polymorphic DNA segment from H. villosa, OPF02757, was obtained. On the basis of cloning and sequencing of OPF02757, two PCR primers were designed and a genome specific PCR marker for H. villosa was established. The PCR marker including 677 bp was localized on all the seven pairs of H. villosa chromosomes. The result of PCR amplification by the primers indicated that there was a specific band of 677 bp in the materials containing H. villosa Chromosome such as T. aestivum-H. villosa addition, T. aestivum-H. villosa substitution, T. aestivum-H. villosa amphidiploid, T. durum-H. villosa amphidiploid and H. villosum from different accessions, and there was no specific band of 677 bp if the materials did not contain H. villosa chromosome, such as T. aestivum, T. durum, Secale cereale, Hordeum vulgare, Thinopyrum elongatum, Thinopyrum intermedium. Therefore, the PCR maker of 677 bp is specific to H. villosa genome, and could be used as molecular marker for detection of chromosomes of H. villosa in wheat. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/12812061/[Development_and_application_of_a_genome_specific_PCR_marker_for_Haynaldia_villosa]_ DB - PRIME DP - Unbound Medicine ER -