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[Role of apoptosis-associated genes and caspase-3 in cisplatin-resistant human ovarian cancer cell lines].
Zhonghua Fu Chan Ke Za Zhi. 2003 Mar; 38(3):158-61.ZF

Abstract

OBJECTIVE

To explore the role of apoptosis-associated genes and caspase-3 activity in cisplatin (DDP)-resistant human ovarian cancer cell lines.

METHODS

The expressions of apoptosis-associated genes (bcl-2, bax, bcl-X(L) and bcl-X(S)) and the activity of caspase-3 were studied by reverse transcription-polymerase chain reaction (RT-PCR) and western blot in the cisplatin-resistant (A2780/DDP, COC1/DDP) and sensitive human ovarian cancer cells (A2780 and COC1). The apoptotic rates of A2780, COC1, A2780/DDP and COC1/DDP were measured with flow cytometry when treated with cisplatin.

RESULTS

The mRNA expressions of bcl-2 and bcl-X(L) in A2780/DDP cells were 1.87 +/- 0.25 and 1.73 +/- 0.15, and significantly higher than those in A2780 cells (P < 0.05), which were 1.48 +/- 0.14 and 1.41 +/- 0.19 respectively. The protein expressions of bcl-2 and bcl-X(L) in A2780/DDP cells were 1.99 +/- 0.11 and 1.69 +/- 0.16, and significantly higher than those in A2780 cells (P < 0.05), which were 1.51 +/- 0.17 and 1.28 +/- 0.11 respectively. The expressions of bcl-2 and bcl-X(L) in COC1/DDP cells were also significantly higher than those in COC1 cells (P < 0.05). While there was no significant difference in expression of bax between A2780/DDP and A2780 as well as between COC1/DDP and COC1. No expression of bcl-X(S) was detected. When cells treated with different concentration of DDP, the caspase-3 activities, apoptotic rates and PARP expressions in A2780/DDP and COC1/DDP were significantly lower than those in A2780 and COC1 (P < 0.05), which showed dose-dependent (P < 0.05).

CONCLUSION

Overexpression of anti-apoptotic genes and decrease of caspase-3 activity may relate to cisplatin resistance in human ovarian cancer cell lines.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, University of Huazhong Science and Technology, Wuhan 430030, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12816691

Citation

Yang, Xiao-kui, et al. "[Role of Apoptosis-associated Genes and Caspase-3 in Cisplatin-resistant Human Ovarian Cancer Cell Lines]." Zhonghua Fu Chan Ke Za Zhi, vol. 38, no. 3, 2003, pp. 158-61.
Yang XK, Xing H, Zheng F, et al. [Role of apoptosis-associated genes and caspase-3 in cisplatin-resistant human ovarian cancer cell lines]. Zhonghua Fu Chan Ke Za Zhi. 2003;38(3):158-61.
Yang, X. K., Xing, H., Zheng, F., Gao, Q. L., Wang, W., Lu, Y. P., & Ma, D. (2003). [Role of apoptosis-associated genes and caspase-3 in cisplatin-resistant human ovarian cancer cell lines]. Zhonghua Fu Chan Ke Za Zhi, 38(3), 158-61.
Yang XK, et al. [Role of Apoptosis-associated Genes and Caspase-3 in Cisplatin-resistant Human Ovarian Cancer Cell Lines]. Zhonghua Fu Chan Ke Za Zhi. 2003;38(3):158-61. PubMed PMID: 12816691.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Role of apoptosis-associated genes and caspase-3 in cisplatin-resistant human ovarian cancer cell lines]. AU - Yang,Xiao-kui, AU - Xing,Hui, AU - Zheng,Fang, AU - Gao,Qing-lei, AU - Wang,Wei, AU - Lu,Yun-ping, AU - Ma,Ding, PY - 2003/6/21/pubmed PY - 2005/1/12/medline PY - 2003/6/21/entrez SP - 158 EP - 61 JF - Zhonghua fu chan ke za zhi JO - Zhonghua Fu Chan Ke Za Zhi VL - 38 IS - 3 N2 - OBJECTIVE: To explore the role of apoptosis-associated genes and caspase-3 activity in cisplatin (DDP)-resistant human ovarian cancer cell lines. METHODS: The expressions of apoptosis-associated genes (bcl-2, bax, bcl-X(L) and bcl-X(S)) and the activity of caspase-3 were studied by reverse transcription-polymerase chain reaction (RT-PCR) and western blot in the cisplatin-resistant (A2780/DDP, COC1/DDP) and sensitive human ovarian cancer cells (A2780 and COC1). The apoptotic rates of A2780, COC1, A2780/DDP and COC1/DDP were measured with flow cytometry when treated with cisplatin. RESULTS: The mRNA expressions of bcl-2 and bcl-X(L) in A2780/DDP cells were 1.87 +/- 0.25 and 1.73 +/- 0.15, and significantly higher than those in A2780 cells (P < 0.05), which were 1.48 +/- 0.14 and 1.41 +/- 0.19 respectively. The protein expressions of bcl-2 and bcl-X(L) in A2780/DDP cells were 1.99 +/- 0.11 and 1.69 +/- 0.16, and significantly higher than those in A2780 cells (P < 0.05), which were 1.51 +/- 0.17 and 1.28 +/- 0.11 respectively. The expressions of bcl-2 and bcl-X(L) in COC1/DDP cells were also significantly higher than those in COC1 cells (P < 0.05). While there was no significant difference in expression of bax between A2780/DDP and A2780 as well as between COC1/DDP and COC1. No expression of bcl-X(S) was detected. When cells treated with different concentration of DDP, the caspase-3 activities, apoptotic rates and PARP expressions in A2780/DDP and COC1/DDP were significantly lower than those in A2780 and COC1 (P < 0.05), which showed dose-dependent (P < 0.05). CONCLUSION: Overexpression of anti-apoptotic genes and decrease of caspase-3 activity may relate to cisplatin resistance in human ovarian cancer cell lines. SN - 0529-567X UR - https://www.unboundmedicine.com/medline/citation/12816691/[Role_of_apoptosis_associated_genes_and_caspase_3_in_cisplatin_resistant_human_ovarian_cancer_cell_lines]_ L2 - http://www.diseaseinfosearch.org/result/5504 DB - PRIME DP - Unbound Medicine ER -