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[Effects of glucose-insulin-potassium cocktail on cardiac myocyte death and post-ischemic recovery cardiac functional recovery: the critical role of insulin].
Zhonghua Nei Ke Za Zhi. 2003 Mar; 42(3):148-52.ZN

Abstract

OBJECTIVE

To study the effect of glucose-insulin-potassium (GIK) cocktail on cardiac myocyte death (i.e., necrosis and apoptosis) and cardiac functional recovery following myocardial ischemia/reperfusion (MI/R), and to further investigate the role of insulin in the GIK-induced cardioprotective effect.

METHODS

Male Sprague-Dawley rats were subjected to 30 min myocardial ischemia followed by reperfusion for 4 h (for cardiac function and cardiomyocyte apoptosis study) or 6 h (for myocardial infarction measurement). Anesthetized rats were randomly treated with continuous infusions of saline, GIK (Glucose: 200 g/L, Insulin: 60 U/L and KCl: 60 mmol/L), GK or insulin at 4 ml.kg(-1).h(-1), beginning 5 min before reperfusion and continuing through the 4-h reperfusion. Arterial blood pressure, ECG and left ventricular pressure were monitored throughout the experiment. Myocardial DNA fragmentation and myocardial infarction were determined at the end of reperfusion.

RESULTS

MI/R caused significant cardiac dysfunction and myocardial death (both necrosis and strong DNA ladder formation). Compared with the vehicle treated rats, the GIK-treated rats showed protection against MI/R injury as evidenced by reduced myocardial infarction [(41.3 +/- 8.3)% vs. (54.4 +/- 10.4)% of vehicle, P < 0.05, n = 10], marked decrease of DNA fragmentation, and improved recovery of cardiac systolic/diastolic function at the end of reperfusion [left ventricular developed pressure: (94 +/- 6) mm Hg vs. (86 +/- 5) mm Hg of vehicle, P < 0.05; +LVdP/dt(max): (2 940 +/- 114) mm Hg/s vs. (2 733 +/- 132) mm Hg/s, P < 0.05; -LVdP/dt(max): (2 629 +/- 156) mm Hg/s vs. (2 463 +/- 133) mm Hg/s, P < 0.05]. Insulin exerted the similar cardioprotective effects with GIK; whereas the rats receiving GK failed to show any significant, cardioprotection against MI/R injury.

CONCLUSIONS

GIK exerts cardioprotective effect against postischemic myocardial injury. Insulin, mainly through its anti-apoptotic effect, plays a critical role in the GIK-elicited myocardial protection in MI/R.

Authors+Show Affiliations

Department of Physiology, The Fourth Military Medical University, Xi'an 710032, China. fgao@fmmu.du.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12816693

Citation

Gao, Feng, et al. "[Effects of Glucose-insulin-potassium Cocktail On Cardiac Myocyte Death and Post-ischemic Recovery Cardiac Functional Recovery: the Critical Role of Insulin]." Zhonghua Nei Ke Za Zhi, vol. 42, no. 3, 2003, pp. 148-52.
Gao F, Shi DW, Wang XM, et al. [Effects of glucose-insulin-potassium cocktail on cardiac myocyte death and post-ischemic recovery cardiac functional recovery: the critical role of insulin]. Zhonghua Nei Ke Za Zhi. 2003;42(3):148-52.
Gao, F., Shi, D. W., Wang, X. M., Dong, L., Wang, Y. M., & Ma, X. L. (2003). [Effects of glucose-insulin-potassium cocktail on cardiac myocyte death and post-ischemic recovery cardiac functional recovery: the critical role of insulin]. Zhonghua Nei Ke Za Zhi, 42(3), 148-52.
Gao F, et al. [Effects of Glucose-insulin-potassium Cocktail On Cardiac Myocyte Death and Post-ischemic Recovery Cardiac Functional Recovery: the Critical Role of Insulin]. Zhonghua Nei Ke Za Zhi. 2003;42(3):148-52. PubMed PMID: 12816693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of glucose-insulin-potassium cocktail on cardiac myocyte death and post-ischemic recovery cardiac functional recovery: the critical role of insulin]. AU - Gao,Feng, AU - Shi,Dong-wei, AU - Wang,Xiao-ming, AU - Dong,Ling, AU - Wang,Yue-min, AU - Ma,Xin-liang, PY - 2003/6/21/pubmed PY - 2004/11/17/medline PY - 2003/6/21/entrez SP - 148 EP - 52 JF - Zhonghua nei ke za zhi JO - Zhonghua Nei Ke Za Zhi VL - 42 IS - 3 N2 - OBJECTIVE: To study the effect of glucose-insulin-potassium (GIK) cocktail on cardiac myocyte death (i.e., necrosis and apoptosis) and cardiac functional recovery following myocardial ischemia/reperfusion (MI/R), and to further investigate the role of insulin in the GIK-induced cardioprotective effect. METHODS: Male Sprague-Dawley rats were subjected to 30 min myocardial ischemia followed by reperfusion for 4 h (for cardiac function and cardiomyocyte apoptosis study) or 6 h (for myocardial infarction measurement). Anesthetized rats were randomly treated with continuous infusions of saline, GIK (Glucose: 200 g/L, Insulin: 60 U/L and KCl: 60 mmol/L), GK or insulin at 4 ml.kg(-1).h(-1), beginning 5 min before reperfusion and continuing through the 4-h reperfusion. Arterial blood pressure, ECG and left ventricular pressure were monitored throughout the experiment. Myocardial DNA fragmentation and myocardial infarction were determined at the end of reperfusion. RESULTS: MI/R caused significant cardiac dysfunction and myocardial death (both necrosis and strong DNA ladder formation). Compared with the vehicle treated rats, the GIK-treated rats showed protection against MI/R injury as evidenced by reduced myocardial infarction [(41.3 +/- 8.3)% vs. (54.4 +/- 10.4)% of vehicle, P < 0.05, n = 10], marked decrease of DNA fragmentation, and improved recovery of cardiac systolic/diastolic function at the end of reperfusion [left ventricular developed pressure: (94 +/- 6) mm Hg vs. (86 +/- 5) mm Hg of vehicle, P < 0.05; +LVdP/dt(max): (2 940 +/- 114) mm Hg/s vs. (2 733 +/- 132) mm Hg/s, P < 0.05; -LVdP/dt(max): (2 629 +/- 156) mm Hg/s vs. (2 463 +/- 133) mm Hg/s, P < 0.05]. Insulin exerted the similar cardioprotective effects with GIK; whereas the rats receiving GK failed to show any significant, cardioprotection against MI/R injury. CONCLUSIONS: GIK exerts cardioprotective effect against postischemic myocardial injury. Insulin, mainly through its anti-apoptotic effect, plays a critical role in the GIK-elicited myocardial protection in MI/R. SN - 0578-1426 UR - https://www.unboundmedicine.com/medline/citation/12816693/[Effects_of_glucose_insulin_potassium_cocktail_on_cardiac_myocyte_death_and_post_ischemic_recovery_cardiac_functional_recovery:_the_critical_role_of_insulin]_ L2 - https://medlineplus.gov/heartattack.html DB - PRIME DP - Unbound Medicine ER -