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In vivo fractional P(i) absorption and NaPi-II mRNA expression in rainbow trout are upregulated by dietary P restriction.
Am J Physiol Regul Integr Comp Physiol. 2003 Oct; 285(4):R770-81.AJ

Abstract

Mammalian type II sodium-phosphate cotransporter (NaPi-II) and inorganic phosphate uptake stimulator (PiUS) genes are upregulated by dietary phosphorus (P) restriction to increase intestinal and renal P transport, but little is known about NaPi-II and PiUS regulation in other vertebrates. We studied the 1). the tissue distribution and dietary regulation of NaPi-II, PiUS, and sodium-glucose cotransporter (SGLT1) mRNA and NaPi-II protein in juvenile rainbow trout (Oncorhynchus mykiss) and 2). effects of dietary P on intestinal Pi absorption in vivo. NaPi-II, PiUS, and SGLT1 mRNA were found in the proximal and distal intestine, pyloric ceca, and kidney. PiUS mRNA was also found in the heart, gill, blood, stomach, liver, skin, and muscle. Tissue distribution of NaPi-II protein correlated with that of NaPi-II mRNA except in gill ionocytes where NaPi-II antibodies recognized related epitopes. Chronic consumption of a low-P diet increased NaPi-II and PiUS but not SGLT1 mRNA abundance in the intestine and kidney. Unlike mammals, there was no detectable shift in tissue or cellular localization of NaPi-II protein in response to dietary P restriction. Regulation of NaPi and PiUS mRNA expression was observed only in fish grown under optimal aqueous oxygen concentrations. In vivo fractional absorption of Pi by the intestine decreased in fish fed high-P diets. Decreases in absorption were less pronounced in fish previously fed low-P diets, suggesting that diet history modulates acute regulation of P absorption. Regulation of dietary Pi absorption in vivo may involve a specific change in intestinal NaPi-II and PiUS gene expression.

Authors+Show Affiliations

Department of Pharmacology and Physiology, New Jersey Medical School, Newark, NJ 07103, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12816744

Citation

Sugiura, Shozo H., et al. "In Vivo Fractional P(i) Absorption and NaPi-II mRNA Expression in Rainbow Trout Are Upregulated By Dietary P Restriction." American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, vol. 285, no. 4, 2003, pp. R770-81.
Sugiura SH, McDaniel NK, Ferraris RP. In vivo fractional P(i) absorption and NaPi-II mRNA expression in rainbow trout are upregulated by dietary P restriction. Am J Physiol Regul Integr Comp Physiol. 2003;285(4):R770-81.
Sugiura, S. H., McDaniel, N. K., & Ferraris, R. P. (2003). In vivo fractional P(i) absorption and NaPi-II mRNA expression in rainbow trout are upregulated by dietary P restriction. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 285(4), R770-81.
Sugiura SH, McDaniel NK, Ferraris RP. In Vivo Fractional P(i) Absorption and NaPi-II mRNA Expression in Rainbow Trout Are Upregulated By Dietary P Restriction. Am J Physiol Regul Integr Comp Physiol. 2003;285(4):R770-81. PubMed PMID: 12816744.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo fractional P(i) absorption and NaPi-II mRNA expression in rainbow trout are upregulated by dietary P restriction. AU - Sugiura,Shozo H, AU - McDaniel,Nichole K, AU - Ferraris,Ronaldo P, Y1 - 2003/06/19/ PY - 2003/6/21/pubmed PY - 2003/10/25/medline PY - 2003/6/21/entrez SP - R770 EP - 81 JF - American journal of physiology. Regulatory, integrative and comparative physiology JO - Am J Physiol Regul Integr Comp Physiol VL - 285 IS - 4 N2 - Mammalian type II sodium-phosphate cotransporter (NaPi-II) and inorganic phosphate uptake stimulator (PiUS) genes are upregulated by dietary phosphorus (P) restriction to increase intestinal and renal P transport, but little is known about NaPi-II and PiUS regulation in other vertebrates. We studied the 1). the tissue distribution and dietary regulation of NaPi-II, PiUS, and sodium-glucose cotransporter (SGLT1) mRNA and NaPi-II protein in juvenile rainbow trout (Oncorhynchus mykiss) and 2). effects of dietary P on intestinal Pi absorption in vivo. NaPi-II, PiUS, and SGLT1 mRNA were found in the proximal and distal intestine, pyloric ceca, and kidney. PiUS mRNA was also found in the heart, gill, blood, stomach, liver, skin, and muscle. Tissue distribution of NaPi-II protein correlated with that of NaPi-II mRNA except in gill ionocytes where NaPi-II antibodies recognized related epitopes. Chronic consumption of a low-P diet increased NaPi-II and PiUS but not SGLT1 mRNA abundance in the intestine and kidney. Unlike mammals, there was no detectable shift in tissue or cellular localization of NaPi-II protein in response to dietary P restriction. Regulation of NaPi and PiUS mRNA expression was observed only in fish grown under optimal aqueous oxygen concentrations. In vivo fractional absorption of Pi by the intestine decreased in fish fed high-P diets. Decreases in absorption were less pronounced in fish previously fed low-P diets, suggesting that diet history modulates acute regulation of P absorption. Regulation of dietary Pi absorption in vivo may involve a specific change in intestinal NaPi-II and PiUS gene expression. SN - 0363-6119 UR - https://www.unboundmedicine.com/medline/citation/12816744/In_vivo_fractional_P_i__absorption_and_NaPi_II_mRNA_expression_in_rainbow_trout_are_upregulated_by_dietary_P_restriction_ L2 - https://journals.physiology.org/doi/10.1152/ajpregu.00127.2003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -