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High level of cFLIP correlates with resistance to death receptor-induced apoptosis in bladder carcinoma cells.
Anticancer Res. 2003 Mar-Apr; 23(2B):1213-8.AR

Abstract

BACKGROUND

The cellular form of FLICE-inhibitory protein (cFLIP) blocks death receptor-induced apoptosis and has been implicated in tumour progression. cFLIP interacts with caspase-8, thereby preventing activation of the caspase cascade. In this study we investigated the endogenous expression of cFLIP and caspase-8 in bladder carcinoma cells in relation to their sensitivity to death receptor-ligation.

MATERIALS AND METHODS

Apoptosis was induced by agonistic anti-CD95 mAbs or recombinant TRAIL and quantified by the TUNEL technique. The relative mRNA expression of cFLIP and caspase-8 was quantified by real-time PCR. Stable expression of cFLIP long (cFLIPL) was obtained by retroviral transduction.

RESULTS

The relative ratio of cFLIP and caspase-8 was directly correlated to resistance to anti-CD95 or TRAIL-mediated apoptosis. Overexpression of cFLIPL shifted the responsiveness towards resistant status.

CONCLUSION

cFLIP is an important determinant of susceptibility to death receptor-induced apoptosis in bladder carcinomas and could function as a prognostic marker for death receptor sensitivity in future immune therapy.

Authors+Show Affiliations

Department of Immunology, Wenner-Gren Institute, Stockholm University, S-106 91 Stockholm, Sweden. gun@imm2.su.seNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12820373

Citation

Jönsson, Gun, et al. "High Level of cFLIP Correlates With Resistance to Death Receptor-induced Apoptosis in Bladder Carcinoma Cells." Anticancer Research, vol. 23, no. 2B, 2003, pp. 1213-8.
Jönsson G, Paulie S, Grandien A. High level of cFLIP correlates with resistance to death receptor-induced apoptosis in bladder carcinoma cells. Anticancer Res. 2003;23(2B):1213-8.
Jönsson, G., Paulie, S., & Grandien, A. (2003). High level of cFLIP correlates with resistance to death receptor-induced apoptosis in bladder carcinoma cells. Anticancer Research, 23(2B), 1213-8.
Jönsson G, Paulie S, Grandien A. High Level of cFLIP Correlates With Resistance to Death Receptor-induced Apoptosis in Bladder Carcinoma Cells. Anticancer Res. 2003 Mar-Apr;23(2B):1213-8. PubMed PMID: 12820373.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High level of cFLIP correlates with resistance to death receptor-induced apoptosis in bladder carcinoma cells. AU - Jönsson,Gun, AU - Paulie,Staffan, AU - Grandien,Alf, PY - 2003/6/25/pubmed PY - 2003/7/26/medline PY - 2003/6/25/entrez SP - 1213 EP - 8 JF - Anticancer research JO - Anticancer Res VL - 23 IS - 2B N2 - BACKGROUND: The cellular form of FLICE-inhibitory protein (cFLIP) blocks death receptor-induced apoptosis and has been implicated in tumour progression. cFLIP interacts with caspase-8, thereby preventing activation of the caspase cascade. In this study we investigated the endogenous expression of cFLIP and caspase-8 in bladder carcinoma cells in relation to their sensitivity to death receptor-ligation. MATERIALS AND METHODS: Apoptosis was induced by agonistic anti-CD95 mAbs or recombinant TRAIL and quantified by the TUNEL technique. The relative mRNA expression of cFLIP and caspase-8 was quantified by real-time PCR. Stable expression of cFLIP long (cFLIPL) was obtained by retroviral transduction. RESULTS: The relative ratio of cFLIP and caspase-8 was directly correlated to resistance to anti-CD95 or TRAIL-mediated apoptosis. Overexpression of cFLIPL shifted the responsiveness towards resistant status. CONCLUSION: cFLIP is an important determinant of susceptibility to death receptor-induced apoptosis in bladder carcinomas and could function as a prognostic marker for death receptor sensitivity in future immune therapy. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/12820373/High_level_of_cFLIP_correlates_with_resistance_to_death_receptor_induced_apoptosis_in_bladder_carcinoma_cells_ L2 - https://medlineplus.gov/bladdercancer.html DB - PRIME DP - Unbound Medicine ER -