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In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L.
J Agric Food Chem. 2003 Jul 02; 51(14):3958-65.JA

Abstract

The present study was designated to evaluate the antimicrobial and antioxidant activities of the essential oil, obtained by using a Clevenger distillation apparatus, water soluble (polar) and water insoluble (nonpolar) subfractions of the methanol extracts from aerial parts of Satureja hortensis L. plants, and methanol extract from calli established from the seeds using Gamborg's B5 basal media supplemented with indole-3-butyric acid (1.0 ppm), 6-benzylaminopurine (N(6)-benzyladenine) (1.0 ppm), and sucrose (2.5%). The antimicrobial test results showed that the essential oil of S. hortensis had great potential antimicrobial activities against all 23 bacteria and 15 fungi and yeast species tested. In contrast, the methanol extract from callus cultures and water soluble subfraction of the methanol extract did not show antimicrobial activities, but the nonpolar subfraction had antibacterial activity against only five out of 23 bacterial species, which were Bacillus subtilis, Enterococcus fecalis, Pseudomonas aeruginosa, Salmonella enteritidis, and Streptococcus pyogenes. Antioxidant studies suggested that the polar subfractions of the methanol extract of intact plant and methanol extract of callus cultures were able to reduce the stable free radical 2,2-diphenyl-1-picrylhydrazyl to the yellow-colored diphenylpicrylhydrazine. In this assay, the strongest effect was observed for the tissue culture extract, with an IC(50) value of 23.76 +/- 0.80 microgram/mL, which could be compared with the synthetic antioxidant agent butylated hydroxytoluene. On the other hand, linoleic acid oxidation was 95% inhibited in the presence of the essential oil while the inhibition was 90% with the chloroform subfraction of the intact plant. The chemical composition of a hydrodistilled essential oil of S. hortensis was analyzed by gas chromatography (GC)/flame ionization detection (FID) and a GC-mass spectrometry system. A total 22 constituents representing 99.9% of the essential oil were identified by GC-FID analaysis. Thymol (29.0%), carvacrol (26.5%), gamma-terpinene (22.6%), and p-cymene (9.3%) were the main components.

Authors+Show Affiliations

Güllüce M
Department of Biology, Faculty of Art and Science, Ataturk University, Erzurum 25240, Turkey. mgulluce@atauni.edu.tr
Sökmen M
No affiliation info available
Daferera D
No affiliation info available
Ağar G
No affiliation info available
Ozkan H
No affiliation info available
Kartal N
No affiliation info available
Polissiou M
No affiliation info available
Sökmen A
No affiliation info available
Sahin F
No affiliation info available

MeSH

Anti-Bacterial AgentsAntifungal AgentsAntioxidantsBacteriaChromatography, GasGas Chromatography-Mass SpectrometryMethanolOils, VolatilePlant ExtractsSatureja

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12822930

Citation

Güllüce, M, et al. "In Vitro Antibacterial, Antifungal, and Antioxidant Activities of the Essential Oil and Methanol Extracts of Herbal Parts and Callus Cultures of Satureja Hortensis L." Journal of Agricultural and Food Chemistry, vol. 51, no. 14, 2003, pp. 3958-65.
Güllüce M, Sökmen M, Daferera D, et al. In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. J Agric Food Chem. 2003;51(14):3958-65.
Güllüce, M., Sökmen, M., Daferera, D., Ağar, G., Ozkan, H., Kartal, N., Polissiou, M., Sökmen, A., & Sahin, F. (2003). In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. Journal of Agricultural and Food Chemistry, 51(14), 3958-65.
Güllüce M, et al. In Vitro Antibacterial, Antifungal, and Antioxidant Activities of the Essential Oil and Methanol Extracts of Herbal Parts and Callus Cultures of Satureja Hortensis L. J Agric Food Chem. 2003 Jul 2;51(14):3958-65. PubMed PMID: 12822930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. AU - Güllüce,M, AU - Sökmen,M, AU - Daferera,D, AU - Ağar,G, AU - Ozkan,H, AU - Kartal,N, AU - Polissiou,M, AU - Sökmen,A, AU - Sahin,F, PY - 2003/6/26/pubmed PY - 2003/8/21/medline PY - 2003/6/26/entrez SP - 3958 EP - 65 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 51 IS - 14 N2 - The present study was designated to evaluate the antimicrobial and antioxidant activities of the essential oil, obtained by using a Clevenger distillation apparatus, water soluble (polar) and water insoluble (nonpolar) subfractions of the methanol extracts from aerial parts of Satureja hortensis L. plants, and methanol extract from calli established from the seeds using Gamborg's B5 basal media supplemented with indole-3-butyric acid (1.0 ppm), 6-benzylaminopurine (N(6)-benzyladenine) (1.0 ppm), and sucrose (2.5%). The antimicrobial test results showed that the essential oil of S. hortensis had great potential antimicrobial activities against all 23 bacteria and 15 fungi and yeast species tested. In contrast, the methanol extract from callus cultures and water soluble subfraction of the methanol extract did not show antimicrobial activities, but the nonpolar subfraction had antibacterial activity against only five out of 23 bacterial species, which were Bacillus subtilis, Enterococcus fecalis, Pseudomonas aeruginosa, Salmonella enteritidis, and Streptococcus pyogenes. Antioxidant studies suggested that the polar subfractions of the methanol extract of intact plant and methanol extract of callus cultures were able to reduce the stable free radical 2,2-diphenyl-1-picrylhydrazyl to the yellow-colored diphenylpicrylhydrazine. In this assay, the strongest effect was observed for the tissue culture extract, with an IC(50) value of 23.76 +/- 0.80 microgram/mL, which could be compared with the synthetic antioxidant agent butylated hydroxytoluene. On the other hand, linoleic acid oxidation was 95% inhibited in the presence of the essential oil while the inhibition was 90% with the chloroform subfraction of the intact plant. The chemical composition of a hydrodistilled essential oil of S. hortensis was analyzed by gas chromatography (GC)/flame ionization detection (FID) and a GC-mass spectrometry system. A total 22 constituents representing 99.9% of the essential oil were identified by GC-FID analaysis. Thymol (29.0%), carvacrol (26.5%), gamma-terpinene (22.6%), and p-cymene (9.3%) were the main components. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/12822930/In_vitro_antibacterial_antifungal_and_antioxidant_activities_of_the_essential_oil_and_methanol_extracts_of_herbal_parts_and_callus_cultures_of_Satureja_hortensis_L_ DB - PRIME DP - Unbound Medicine ER -