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Hop (Humulus lupulus L.) proanthocyanidins characterized by mass spectrometry, acid catalysis, and gel permeation chromatography.
J Agric Food Chem. 2003 Jul 02; 51(14):4101-10.JA

Abstract

Proanthocyanidins extracted from hops (Humulus lupulus L. cv. Willamette) were subjected to Sephadex LH-20 column chromatography using a step gradient of methanol, water, and acetone. The resulting fractions were analyzed by acid catalysis, electrospray ionization and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and gel permeation chromatography (GPC). The proanthocyanidins contained catechin and epicatechin as monomers and as terminal and extension units. Epigallocatechin was found as extension units. The mean degree of polymerization (mDP) of the crude proanthocyanidins was 7.8, but heptamers were the largest oligomers visible in mass spectra of the whole. In the last-eluted fraction (mDP = 22.2), polymers as large as 20-mers were detected by MALDI-TOF-MS, demonstrating the effectiveness of prior separation in improving MS detection. GPC data correlated well with acid catalysis results, confirming the presence of large polymers that were not detected by MS.

Authors+Show Affiliations

Department of Chemistry, Oregon State University, Corvallis, Oregon 97331, USA. Alan.Taylor@orst.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12822954

Citation

Taylor, Alan W., et al. "Hop (Humulus Lupulus L.) Proanthocyanidins Characterized By Mass Spectrometry, Acid Catalysis, and Gel Permeation Chromatography." Journal of Agricultural and Food Chemistry, vol. 51, no. 14, 2003, pp. 4101-10.
Taylor AW, Barofsky E, Kennedy JA, et al. Hop (Humulus lupulus L.) proanthocyanidins characterized by mass spectrometry, acid catalysis, and gel permeation chromatography. J Agric Food Chem. 2003;51(14):4101-10.
Taylor, A. W., Barofsky, E., Kennedy, J. A., & Deinzer, M. L. (2003). Hop (Humulus lupulus L.) proanthocyanidins characterized by mass spectrometry, acid catalysis, and gel permeation chromatography. Journal of Agricultural and Food Chemistry, 51(14), 4101-10.
Taylor AW, et al. Hop (Humulus Lupulus L.) Proanthocyanidins Characterized By Mass Spectrometry, Acid Catalysis, and Gel Permeation Chromatography. J Agric Food Chem. 2003 Jul 2;51(14):4101-10. PubMed PMID: 12822954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hop (Humulus lupulus L.) proanthocyanidins characterized by mass spectrometry, acid catalysis, and gel permeation chromatography. AU - Taylor,Alan W, AU - Barofsky,Elizabeth, AU - Kennedy,James A, AU - Deinzer,Max L, PY - 2003/6/26/pubmed PY - 2003/8/21/medline PY - 2003/6/26/entrez SP - 4101 EP - 10 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 51 IS - 14 N2 - Proanthocyanidins extracted from hops (Humulus lupulus L. cv. Willamette) were subjected to Sephadex LH-20 column chromatography using a step gradient of methanol, water, and acetone. The resulting fractions were analyzed by acid catalysis, electrospray ionization and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and gel permeation chromatography (GPC). The proanthocyanidins contained catechin and epicatechin as monomers and as terminal and extension units. Epigallocatechin was found as extension units. The mean degree of polymerization (mDP) of the crude proanthocyanidins was 7.8, but heptamers were the largest oligomers visible in mass spectra of the whole. In the last-eluted fraction (mDP = 22.2), polymers as large as 20-mers were detected by MALDI-TOF-MS, demonstrating the effectiveness of prior separation in improving MS detection. GPC data correlated well with acid catalysis results, confirming the presence of large polymers that were not detected by MS. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/12822954/Hop__Humulus_lupulus_L___proanthocyanidins_characterized_by_mass_spectrometry_acid_catalysis_and_gel_permeation_chromatography_ L2 - https://doi.org/10.1021/jf0340409 DB - PRIME DP - Unbound Medicine ER -