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Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom. Identification of the origin of a new viper population based on ammodytin I1 heterogeneity.
Eur J Biochem. 2003 Jul; 270(13):2697-706.EJ

Abstract

We used a PCR-based method to determine the genomic DNA sequences encoding phospholipases A2 (PLA2s) from the venoms of Vipera aspis aspis (V. a. aspis), Vipera aspis zinnikeri (V. a. zinnikeri), Vipera berus berus (V. b. berus) and a neurotoxic V. a. aspis snake (neurotoxic V. a. aspis) from a population responsible for unusual neurotoxic envenomations in south-east France. We sequenced five groups of genes, each corresponding to a different PLA2. The genes encoding the A and B chains of vaspin from the neurotoxic V. a. aspis, PLA2-I from V. a. zinnikeri, and the anticoagulant PLA2 from V. b. berus are described here. Single nucleotide differences leading to amino-acid substitutions were observed both between genes encoding the same PLA2 and between genes encoding different PLA2s. These differences were clustered in exons 3 and 5, potentially altering the biological activities of PLA2. The distribution and characteristics of the PLA2 genes differed according to the species or subspecies. We characterized for the first time genes encoding neurotoxins from the V. a. aspis and V. b. berus snakes of central France. Genes encoding ammodytins I1 and I2, described previously in Vipera ammodytes ammodytes (V. am. ammodytes), were also present in V. a. aspis and V. b. berus. Three different ammodytin I1 gene sequences were characterized: one from V. b. berus, the second from V. a. aspis, V. a. zinnikeri and the neurotoxic V. a. aspis, and the third from the neurotoxic V. a. aspis. This third sequence was identical with the reported sequence of the V. am. ammodytes ammodytin I1 gene. Genes encoding monomeric neurotoxins of V. am. ammodytes venom, ammodytoxins A, B and C, and the Bov-B LINE retroposon, a phylogenetic marker found in V. am. ammodytes genome, were identified in the genome of the neurotoxic V. a. aspis. These results suggest that the population of neurotoxic V. a. aspis snakes from south-east France may have resulted from interbreeding between V. a. aspis and V. am. ammodytes.

Authors+Show Affiliations

Guillemin I
Unité des Venins, Institut Pasteur, Paris, France.
Bouchier C
No affiliation info available
Garrigues T
No affiliation info available
Wisner A
No affiliation info available
Choumet V
No affiliation info available

MeSH

Amino Acid SequenceAnimalsBase SequenceFranceMolecular Sequence DataPhospholipases APhospholipases A2Sequence AlignmentViper VenomsViperidae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12823540

Citation

Guillemin, Isabelle, et al. "Sequences and Structural Organization of Phospholipase A2 Genes From Vipera Aspis Aspis, V. Aspis Zinnikeri and Vipera Berus Berus Venom. Identification of the Origin of a New Viper Population Based On Ammodytin I1 Heterogeneity." European Journal of Biochemistry, vol. 270, no. 13, 2003, pp. 2697-706.
Guillemin I, Bouchier C, Garrigues T, et al. Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom. Identification of the origin of a new viper population based on ammodytin I1 heterogeneity. Eur J Biochem. 2003;270(13):2697-706.
Guillemin, I., Bouchier, C., Garrigues, T., Wisner, A., & Choumet, V. (2003). Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom. Identification of the origin of a new viper population based on ammodytin I1 heterogeneity. European Journal of Biochemistry, 270(13), 2697-706.
Guillemin I, et al. Sequences and Structural Organization of Phospholipase A2 Genes From Vipera Aspis Aspis, V. Aspis Zinnikeri and Vipera Berus Berus Venom. Identification of the Origin of a New Viper Population Based On Ammodytin I1 Heterogeneity. Eur J Biochem. 2003;270(13):2697-706. PubMed PMID: 12823540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequences and structural organization of phospholipase A2 genes from Vipera aspis aspis, V. aspis zinnikeri and Vipera berus berus venom. Identification of the origin of a new viper population based on ammodytin I1 heterogeneity. AU - Guillemin,Isabelle, AU - Bouchier,Christiane, AU - Garrigues,Thomas, AU - Wisner,Anne, AU - Choumet,Valérie, PY - 2003/6/26/pubmed PY - 2003/8/30/medline PY - 2003/6/26/entrez SP - 2697 EP - 706 JF - European journal of biochemistry JO - Eur J Biochem VL - 270 IS - 13 N2 - We used a PCR-based method to determine the genomic DNA sequences encoding phospholipases A2 (PLA2s) from the venoms of Vipera aspis aspis (V. a. aspis), Vipera aspis zinnikeri (V. a. zinnikeri), Vipera berus berus (V. b. berus) and a neurotoxic V. a. aspis snake (neurotoxic V. a. aspis) from a population responsible for unusual neurotoxic envenomations in south-east France. We sequenced five groups of genes, each corresponding to a different PLA2. The genes encoding the A and B chains of vaspin from the neurotoxic V. a. aspis, PLA2-I from V. a. zinnikeri, and the anticoagulant PLA2 from V. b. berus are described here. Single nucleotide differences leading to amino-acid substitutions were observed both between genes encoding the same PLA2 and between genes encoding different PLA2s. These differences were clustered in exons 3 and 5, potentially altering the biological activities of PLA2. The distribution and characteristics of the PLA2 genes differed according to the species or subspecies. We characterized for the first time genes encoding neurotoxins from the V. a. aspis and V. b. berus snakes of central France. Genes encoding ammodytins I1 and I2, described previously in Vipera ammodytes ammodytes (V. am. ammodytes), were also present in V. a. aspis and V. b. berus. Three different ammodytin I1 gene sequences were characterized: one from V. b. berus, the second from V. a. aspis, V. a. zinnikeri and the neurotoxic V. a. aspis, and the third from the neurotoxic V. a. aspis. This third sequence was identical with the reported sequence of the V. am. ammodytes ammodytin I1 gene. Genes encoding monomeric neurotoxins of V. am. ammodytes venom, ammodytoxins A, B and C, and the Bov-B LINE retroposon, a phylogenetic marker found in V. am. ammodytes genome, were identified in the genome of the neurotoxic V. a. aspis. These results suggest that the population of neurotoxic V. a. aspis snakes from south-east France may have resulted from interbreeding between V. a. aspis and V. am. ammodytes. SN - 0014-2956 UR - https://www.unboundmedicine.com/medline/citation/12823540/Sequences_and_structural_organization_of_phospholipase_A2_genes_from_Vipera_aspis_aspis_V__aspis_zinnikeri_and_Vipera_berus_berus_venom__Identification_of_the_origin_of_a_new_viper_population_based_on_ammodytin_I1_heterogeneity_ DB - PRIME DP - Unbound Medicine ER -