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Differential dendritic shrinkage of alpha and beta retinal ganglion cells in cats with chronic glaucoma.
Invest Ophthalmol Vis Sci. 2003 Jul; 44(7):3005-10.IO

Abstract

PURPOSE

To study changes in the dendritic morphology of retinal ganglion cells (RGCs) in cats with experimental chronic glaucoma.

METHODS

Chronic elevation of intraocular pressure (IOP) was produced by injecting endogenous ghost red blood cells into the unilateral anterior chamber of the feline eyes for 1 month. The morphologic features of retrograde-labeled RGCs by bilateral injection of horseradish peroxidase (HRP) into layers A and Aa1 of the lateral geniculate nucleus (LGN) were examined and compared between the normal and glaucomatous eyes. Nissl staining was used for measuring the change in cell density in the retina and the LGN.

RESULTS

Quantitative analysis of 720 labeled alpha and beta type RGCs showed that the cell density, body size, maximum dendritic field radius, total dendritic length, and number of branch bifurcations of dendrites decreased significantly in glaucomatous eyes compared with normal ones. The cell loss and shrinkage of dendrites in alpha type ganglion cells in the retina was more pronounced than that in beta type cells. The cell density of all kinds of cells in the retina and LGN monotonically declined with time while IOP was elevated, and cell loss was more significant in large cells than in small ones.

CONCLUSION

Progressive cell loss and dendritic damage by chronic elevation of IOP in RGCs and LGN cells are more pronounced in the Y-channel (large cells) than the X-channel (small cells) in feline glaucomatous eyes. The dendritic structure changes and corresponding physiological deficits of RGCs occur before cell death and thus may provide an opportunity for clinical treatment.

Authors+Show Affiliations

Vision Research Laboratory, Center for Brain Science Research and State Key Laboratory of Medical Neurobiology, School of Life Sciences, Fudan University, Shanghai, China. tdshou@fudan.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12824245

Citation

Shou, Tiande, et al. "Differential Dendritic Shrinkage of Alpha and Beta Retinal Ganglion Cells in Cats With Chronic Glaucoma." Investigative Ophthalmology & Visual Science, vol. 44, no. 7, 2003, pp. 3005-10.
Shou T, Liu J, Wang W, et al. Differential dendritic shrinkage of alpha and beta retinal ganglion cells in cats with chronic glaucoma. Invest Ophthalmol Vis Sci. 2003;44(7):3005-10.
Shou, T., Liu, J., Wang, W., Zhou, Y., & Zhao, K. (2003). Differential dendritic shrinkage of alpha and beta retinal ganglion cells in cats with chronic glaucoma. Investigative Ophthalmology & Visual Science, 44(7), 3005-10.
Shou T, et al. Differential Dendritic Shrinkage of Alpha and Beta Retinal Ganglion Cells in Cats With Chronic Glaucoma. Invest Ophthalmol Vis Sci. 2003;44(7):3005-10. PubMed PMID: 12824245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential dendritic shrinkage of alpha and beta retinal ganglion cells in cats with chronic glaucoma. AU - Shou,Tiande, AU - Liu,Jie, AU - Wang,Wei, AU - Zhou,Yifeng, AU - Zhao,Kanxing, PY - 2003/6/26/pubmed PY - 2003/8/5/medline PY - 2003/6/26/entrez SP - 3005 EP - 10 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 44 IS - 7 N2 - PURPOSE: To study changes in the dendritic morphology of retinal ganglion cells (RGCs) in cats with experimental chronic glaucoma. METHODS: Chronic elevation of intraocular pressure (IOP) was produced by injecting endogenous ghost red blood cells into the unilateral anterior chamber of the feline eyes for 1 month. The morphologic features of retrograde-labeled RGCs by bilateral injection of horseradish peroxidase (HRP) into layers A and Aa1 of the lateral geniculate nucleus (LGN) were examined and compared between the normal and glaucomatous eyes. Nissl staining was used for measuring the change in cell density in the retina and the LGN. RESULTS: Quantitative analysis of 720 labeled alpha and beta type RGCs showed that the cell density, body size, maximum dendritic field radius, total dendritic length, and number of branch bifurcations of dendrites decreased significantly in glaucomatous eyes compared with normal ones. The cell loss and shrinkage of dendrites in alpha type ganglion cells in the retina was more pronounced than that in beta type cells. The cell density of all kinds of cells in the retina and LGN monotonically declined with time while IOP was elevated, and cell loss was more significant in large cells than in small ones. CONCLUSION: Progressive cell loss and dendritic damage by chronic elevation of IOP in RGCs and LGN cells are more pronounced in the Y-channel (large cells) than the X-channel (small cells) in feline glaucomatous eyes. The dendritic structure changes and corresponding physiological deficits of RGCs occur before cell death and thus may provide an opportunity for clinical treatment. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/12824245/Differential_dendritic_shrinkage_of_alpha_and_beta_retinal_ganglion_cells_in_cats_with_chronic_glaucoma_ DB - PRIME DP - Unbound Medicine ER -