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Comparison of early ileal invasion by Salmonella enterica serovars Choleraesuis and Typhimurium.
Vet Pathol. 2003 Jul; 40(4):371-5.VP

Abstract

The mechanisms of Salmonella serovar-host specificity are not well defined. Pig ileal loops were used to compare phenotypic differences in early cellular invasion between non-host-adapted Salmonella serovar Typhimurium (SsT) and host-adapted Salmonella serovar Choleraesuis (SsC). By 10 minutes postinoculation, both serovars invaded a small number of M cells, enterocytes, and goblet cells. Multiple SsC organisms (up to 6 per cell) simultaneously invaded M cells, whereas SsT often invaded as one to two organisms per M cell. Internalization of both serovars resulted in vacuoles containing a single bacterium. The follicle-associated epithelium (FAE) of SsC-inoculated loops responded with more filopodia and lamellipodia although exhibiting less cell swelling than SsT. Additionally, SsT showed an enhanced affinity for sites of cell extrusion compared with SsC at 60 minutes. These results suggest: 1) both SsC and SsT exhibit non-cell-specific invasion as early as 10 minutes postinoculation, 2) Salmonella serovars exhibit differences in early invasion of FAE and M cells, and 3) cells undergoing extrusion may provide a site for preferential adherence by SsT and SsC.

Authors+Show Affiliations

Department of Veterinary Microbiology, Iowa State University, Ames, IA 50010, USA.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12824508

Citation

Meyerholz, D K., and T J. Stabel. "Comparison of Early Ileal Invasion By Salmonella Enterica Serovars Choleraesuis and Typhimurium." Veterinary Pathology, vol. 40, no. 4, 2003, pp. 371-5.
Meyerholz DK, Stabel TJ. Comparison of early ileal invasion by Salmonella enterica serovars Choleraesuis and Typhimurium. Vet Pathol. 2003;40(4):371-5.
Meyerholz, D. K., & Stabel, T. J. (2003). Comparison of early ileal invasion by Salmonella enterica serovars Choleraesuis and Typhimurium. Veterinary Pathology, 40(4), 371-5.
Meyerholz DK, Stabel TJ. Comparison of Early Ileal Invasion By Salmonella Enterica Serovars Choleraesuis and Typhimurium. Vet Pathol. 2003;40(4):371-5. PubMed PMID: 12824508.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of early ileal invasion by Salmonella enterica serovars Choleraesuis and Typhimurium. AU - Meyerholz,D K, AU - Stabel,T J, PY - 2003/6/26/pubmed PY - 2003/10/15/medline PY - 2003/6/26/entrez SP - 371 EP - 5 JF - Veterinary pathology JO - Vet Pathol VL - 40 IS - 4 N2 - The mechanisms of Salmonella serovar-host specificity are not well defined. Pig ileal loops were used to compare phenotypic differences in early cellular invasion between non-host-adapted Salmonella serovar Typhimurium (SsT) and host-adapted Salmonella serovar Choleraesuis (SsC). By 10 minutes postinoculation, both serovars invaded a small number of M cells, enterocytes, and goblet cells. Multiple SsC organisms (up to 6 per cell) simultaneously invaded M cells, whereas SsT often invaded as one to two organisms per M cell. Internalization of both serovars resulted in vacuoles containing a single bacterium. The follicle-associated epithelium (FAE) of SsC-inoculated loops responded with more filopodia and lamellipodia although exhibiting less cell swelling than SsT. Additionally, SsT showed an enhanced affinity for sites of cell extrusion compared with SsC at 60 minutes. These results suggest: 1) both SsC and SsT exhibit non-cell-specific invasion as early as 10 minutes postinoculation, 2) Salmonella serovars exhibit differences in early invasion of FAE and M cells, and 3) cells undergoing extrusion may provide a site for preferential adherence by SsT and SsC. SN - 0300-9858 UR - https://www.unboundmedicine.com/medline/citation/12824508/Comparison_of_early_ileal_invasion_by_Salmonella_enterica_serovars_Choleraesuis_and_Typhimurium_ L2 - https://journals.sagepub.com/doi/10.1354/vp.40-4-371?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -