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Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases.
Cytometry B Clin Cytom. 2003 Jul; 54(1):19-27.CB

Abstract

BACKGROUND

The integrin CD103 is preferentially expressed on intraepithelial T lymphocytes, and cells expressing this integrin may play a regulatory role in the microenvironment of the epithelial cell layer.

METHODS

The relative number of CD103(+)/CD4(+) T cells in the bronchoalveolar lavage was significantly elevated in all patients diagnosed with interstitial lung diseases compared with patients with other non-fibrotic disorders of the lung.

RESULTS

Analysis by flow cytometry showed that the CD103(+) and the CD103(-) subpopulations were memory T cells based on the high expression of CD45RO(+). However, the CD103(+)/CD4(+) T cells were CD25(low), CD27(-), CD28(low), and CD62L(-), whereas the CD103(-)/CD4(+) T cells expressed CD25 and CD62L and were CD27(high) and CD28(high). In addition, the CD103(+)/CD4(+) T cells expressed significantly higher quantities of VLA-1 and CD101 than did CD103(-)/CD4(+) T cells. Reverse transcriptase polymerase chain reaction analysis of purified CD103(+) and CD103(-) CD4(+) T cells showed production of tumor necrosis factor (TNF) alpha-R-1 (p55), TNF-alpha-R-2 (p75), interferon gamma, interleukin-10, and TNF-alpha mRNA in both subpopulations. No interleukin-4 mRNA was detected in either subpopulation.

CONCLUSIONS

CD103(+)/CD4(+) T cells represent a T-helper 1-like subpopulation in human lungs with a distinct effector phenotype. Despite the lack of CD27 and the low CD25 and CD28 expression, these cells show a high degree of activation. These results suggest that CD103 expressing CD4 T cells in the lung are continuously activated, long-living cells.

Authors+Show Affiliations

Ottawa Health Research Institute, Ottawa, Ontario, Canada. rbraun@ohri.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12827664

Citation

Braun, Ruedi K., et al. "Phenotypic and Molecular Characterization of CD103+ CD4+ T Cells in Bronchoalveolar Lavage From Patients With Interstitial Lung Diseases." Cytometry. Part B, Clinical Cytometry, vol. 54, no. 1, 2003, pp. 19-27.
Braun RK, Foerster M, Grahmann PR, et al. Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases. Cytometry B Clin Cytom. 2003;54(1):19-27.
Braun, R. K., Foerster, M., Grahmann, P. R., Haefner, D., Workalemahu, G., & Kroegel, C. (2003). Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases. Cytometry. Part B, Clinical Cytometry, 54(1), 19-27.
Braun RK, et al. Phenotypic and Molecular Characterization of CD103+ CD4+ T Cells in Bronchoalveolar Lavage From Patients With Interstitial Lung Diseases. Cytometry B Clin Cytom. 2003;54(1):19-27. PubMed PMID: 12827664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases. AU - Braun,Ruedi K, AU - Foerster,Martin, AU - Grahmann,P Reinhard, AU - Haefner,Daniela, AU - Workalemahu,Grefachew, AU - Kroegel,Claus, PY - 2003/6/27/pubmed PY - 2004/2/12/medline PY - 2003/6/27/entrez SP - 19 EP - 27 JF - Cytometry. Part B, Clinical cytometry JO - Cytometry B Clin Cytom VL - 54 IS - 1 N2 - BACKGROUND: The integrin CD103 is preferentially expressed on intraepithelial T lymphocytes, and cells expressing this integrin may play a regulatory role in the microenvironment of the epithelial cell layer. METHODS: The relative number of CD103(+)/CD4(+) T cells in the bronchoalveolar lavage was significantly elevated in all patients diagnosed with interstitial lung diseases compared with patients with other non-fibrotic disorders of the lung. RESULTS: Analysis by flow cytometry showed that the CD103(+) and the CD103(-) subpopulations were memory T cells based on the high expression of CD45RO(+). However, the CD103(+)/CD4(+) T cells were CD25(low), CD27(-), CD28(low), and CD62L(-), whereas the CD103(-)/CD4(+) T cells expressed CD25 and CD62L and were CD27(high) and CD28(high). In addition, the CD103(+)/CD4(+) T cells expressed significantly higher quantities of VLA-1 and CD101 than did CD103(-)/CD4(+) T cells. Reverse transcriptase polymerase chain reaction analysis of purified CD103(+) and CD103(-) CD4(+) T cells showed production of tumor necrosis factor (TNF) alpha-R-1 (p55), TNF-alpha-R-2 (p75), interferon gamma, interleukin-10, and TNF-alpha mRNA in both subpopulations. No interleukin-4 mRNA was detected in either subpopulation. CONCLUSIONS: CD103(+)/CD4(+) T cells represent a T-helper 1-like subpopulation in human lungs with a distinct effector phenotype. Despite the lack of CD27 and the low CD25 and CD28 expression, these cells show a high degree of activation. These results suggest that CD103 expressing CD4 T cells in the lung are continuously activated, long-living cells. SN - 1552-4949 UR - https://www.unboundmedicine.com/medline/citation/12827664/Phenotypic_and_molecular_characterization_of_CD103+_CD4+_T_cells_in_bronchoalveolar_lavage_from_patients_with_interstitial_lung_diseases_ DB - PRIME DP - Unbound Medicine ER -