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Expression of inducible nitric oxide synthase in thyroid neoplasms: immunohistochemical and molecular analysis.
Pathol Int. 2003 Jul; 53(7):434-9.PI

Abstract

To understand the role of inducible nitric oxide synthase (iNOS) in thyroid tumorigenesis, immunohistochemical staining of 36 surgical specimens of thyroid neoplasm that consisted of seven follicular adenomas, 12 papillary carcinomas, seven follicular carcinomas, five medullary carcinomas, and five anaplastic carcinomas were analyzed. In addition, 20 specimens of normal thyroid were used as control samples. Reverse transcription-polymerase chain reaction and western blot analysis were also performed using a normal thyroid and a representative papillary carcinoma case. The intensity and proportion of the immunostained tumor cells were graded semiquantitatively. The grades of the intensity and the proportion were then summed to provide an immunohistochemical score. There was a variation in the staining intensity and proportion. The iNOS expression was low in normal follicular epithelia. Inducible nitric oxide synthase is present in the majority of thyroid tumor cells, including follicular adenomas, papillary carcinomas, follicular carcinomas, medullary carcinomas, and anaplastic carcinomas. Relatively low expression was shown in follicular neoplasms. Only a few inflammatory cells in the stroma were immunoreactive. These results suggest that iNOS may have a role in tumorigenesis, and iNOS in human thyroid carcinoma is mostly derived from tumor cells not from macrophages.

Authors+Show Affiliations

Department of Nuclear Medicine, Inha University College of Medicine, Incheon, Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12828608

Citation

Choe, Wonsick, et al. "Expression of Inducible Nitric Oxide Synthase in Thyroid Neoplasms: Immunohistochemical and Molecular Analysis." Pathology International, vol. 53, no. 7, 2003, pp. 434-9.
Choe W, Kim S, Hwang TS, et al. Expression of inducible nitric oxide synthase in thyroid neoplasms: immunohistochemical and molecular analysis. Pathol Int. 2003;53(7):434-9.
Choe, W., Kim, S., Hwang, T. S., & Lee, S. S. (2003). Expression of inducible nitric oxide synthase in thyroid neoplasms: immunohistochemical and molecular analysis. Pathology International, 53(7), 434-9.
Choe W, et al. Expression of Inducible Nitric Oxide Synthase in Thyroid Neoplasms: Immunohistochemical and Molecular Analysis. Pathol Int. 2003;53(7):434-9. PubMed PMID: 12828608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of inducible nitric oxide synthase in thyroid neoplasms: immunohistochemical and molecular analysis. AU - Choe,Wonsick, AU - Kim,Sungeun, AU - Hwang,Tae Sook, AU - Lee,Seung Sook, PY - 2003/6/28/pubmed PY - 2004/3/31/medline PY - 2003/6/28/entrez SP - 434 EP - 9 JF - Pathology international JO - Pathol Int VL - 53 IS - 7 N2 - To understand the role of inducible nitric oxide synthase (iNOS) in thyroid tumorigenesis, immunohistochemical staining of 36 surgical specimens of thyroid neoplasm that consisted of seven follicular adenomas, 12 papillary carcinomas, seven follicular carcinomas, five medullary carcinomas, and five anaplastic carcinomas were analyzed. In addition, 20 specimens of normal thyroid were used as control samples. Reverse transcription-polymerase chain reaction and western blot analysis were also performed using a normal thyroid and a representative papillary carcinoma case. The intensity and proportion of the immunostained tumor cells were graded semiquantitatively. The grades of the intensity and the proportion were then summed to provide an immunohistochemical score. There was a variation in the staining intensity and proportion. The iNOS expression was low in normal follicular epithelia. Inducible nitric oxide synthase is present in the majority of thyroid tumor cells, including follicular adenomas, papillary carcinomas, follicular carcinomas, medullary carcinomas, and anaplastic carcinomas. Relatively low expression was shown in follicular neoplasms. Only a few inflammatory cells in the stroma were immunoreactive. These results suggest that iNOS may have a role in tumorigenesis, and iNOS in human thyroid carcinoma is mostly derived from tumor cells not from macrophages. SN - 1320-5463 UR - https://www.unboundmedicine.com/medline/citation/12828608/Expression_of_inducible_nitric_oxide_synthase_in_thyroid_neoplasms:_immunohistochemical_and_molecular_analysis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1320-5463&date=2003&volume=53&issue=7&spage=434 DB - PRIME DP - Unbound Medicine ER -