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Crystallization and preliminary X-ray crystallographic analysis of quinolinate phosphoribosyltransferase of Helicobacter pylori.
Acta Crystallogr D Biol Crystallogr. 2003 Jul; 59(Pt 7):1265-6.AC

Abstract

Quinolinic acid phosphoribosyltransferase (NadC; EC 2.4.2.19) is the key enzyme of NAD(+) biosynthesis in both prokaryotes and eukaryotes. NadC catalyzes the decarboxylation of quinolinic acid (QA) to produce nicotinic acid mononucleotide (NAMN), an intermediate in NAD synthesis. NadCs of Helicobacter pylori appeared to be a hexamer during the purification procedure. Three different complexes of NadC, with QA, NAMN and phthalic acid (PA), an analogue of QA, were crystallized at 294 +/- 1 K using the hanging-drop vapour-diffusion method. The QA complex crystal was found to belong to space group P4(1)2(1)2, with unit-cell parameters a = b = 148.8, c = 145.7 A, alpha = beta = gamma = 90 degrees. Diffraction data were collected from the NadC-substrate and NadC-substrate analogue complexes to resolutions of 2.3 A (QA), 2.8 A (PA) and 3.3 A (NAMN) using synchrotron X-ray radiation.

Authors+Show Affiliations

Kim MK
Department of Life Science, Kwangju Institute of Science and Technology, Gwangju 500-712, South Korea.
Kim YS
No affiliation info available
Rho SH
No affiliation info available
Im YJ
No affiliation info available
Lee JH
No affiliation info available
Kang GB
No affiliation info available
Eom SH
No affiliation info available

MeSH

Cloning, MolecularCrystallizationCrystallography, X-RayHelicobacter pyloriNicotinamide MononucleotidePentosyltransferasesPhthalic AcidsProtein BindingQuinolinic Acid

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12832780

Citation

Kim, Mun-Kyoung, et al. "Crystallization and Preliminary X-ray Crystallographic Analysis of Quinolinate Phosphoribosyltransferase of Helicobacter Pylori." Acta Crystallographica. Section D, Biological Crystallography, vol. 59, no. Pt 7, 2003, pp. 1265-6.
Kim MK, Kim YS, Rho SH, et al. Crystallization and preliminary X-ray crystallographic analysis of quinolinate phosphoribosyltransferase of Helicobacter pylori. Acta Crystallogr D Biol Crystallogr. 2003;59(Pt 7):1265-6.
Kim, M. K., Kim, Y. S., Rho, S. H., Im, Y. J., Lee, J. H., Kang, G. B., & Eom, S. H. (2003). Crystallization and preliminary X-ray crystallographic analysis of quinolinate phosphoribosyltransferase of Helicobacter pylori. Acta Crystallographica. Section D, Biological Crystallography, 59(Pt 7), 1265-6.
Kim MK, et al. Crystallization and Preliminary X-ray Crystallographic Analysis of Quinolinate Phosphoribosyltransferase of Helicobacter Pylori. Acta Crystallogr D Biol Crystallogr. 2003;59(Pt 7):1265-6. PubMed PMID: 12832780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystallization and preliminary X-ray crystallographic analysis of quinolinate phosphoribosyltransferase of Helicobacter pylori. AU - Kim,Mun-Kyoung, AU - Kim,Yun Sik, AU - Rho,Seong-Hwan, AU - Im,Young Jun, AU - Lee,Jun Hyuck, AU - Kang,Gil Bu, AU - Eom,Soo Hyun, Y1 - 2003/06/27/ PY - 2003/02/11/received PY - 2003/04/22/accepted PY - 2003/7/2/pubmed PY - 2004/3/26/medline PY - 2003/7/2/entrez SP - 1265 EP - 6 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 59 IS - Pt 7 N2 - Quinolinic acid phosphoribosyltransferase (NadC; EC 2.4.2.19) is the key enzyme of NAD(+) biosynthesis in both prokaryotes and eukaryotes. NadC catalyzes the decarboxylation of quinolinic acid (QA) to produce nicotinic acid mononucleotide (NAMN), an intermediate in NAD synthesis. NadCs of Helicobacter pylori appeared to be a hexamer during the purification procedure. Three different complexes of NadC, with QA, NAMN and phthalic acid (PA), an analogue of QA, were crystallized at 294 +/- 1 K using the hanging-drop vapour-diffusion method. The QA complex crystal was found to belong to space group P4(1)2(1)2, with unit-cell parameters a = b = 148.8, c = 145.7 A, alpha = beta = gamma = 90 degrees. Diffraction data were collected from the NadC-substrate and NadC-substrate analogue complexes to resolutions of 2.3 A (QA), 2.8 A (PA) and 3.3 A (NAMN) using synchrotron X-ray radiation. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/12832780/Crystallization_and_preliminary_X_ray_crystallographic_analysis_of_quinolinate_phosphoribosyltransferase_of_Helicobacter_pylori_ DB - PRIME DP - Unbound Medicine ER -