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Isolation and characterization of S genome specific sequences from Aegilops sect. sitopsis species.
Genome. 2003 Jun; 46(3):478-89.G

Abstract

Three S genome specific sequences were isolated from Aegilops sect. sitopsis species using different experimental approaches. Two clones, UTV86 and UTV39, were isolated from a partial genomic library obtained from DNA of Aegilops sharonensis, whereas a third clone, UTV5, was isolated from Aegilops speltoides. The three clones were characterized by sequencing, analysis of methylation, and sequence organization and abundance in some Aegilops and Triticum species. The clones UTV39 and UTV5 belong to the same family of tandem repeated sequences and showed high homology with a sequence already present in nucleotide databases. The UTV86 clone from Ae. sharonensis corresponded to an interspersed low frequency repeated sequence and did not show any significant homology with reported sequences. Southern hybridization experiments, using the cloned sequences as probes, detected polymorphism in the restriction patterns of all the five Aegilops species in section sitopsis. Aegilops speltoides showed the most divergent hybridization pattern. A close relationship was detected between the S genome of Ae. speltoides and the G genome of the wild Triticum timopheevii. In situ hybridization revealed a telomeric and (or) subtelomeric location of the sequences UTV39 and UTV5.

Authors+Show Affiliations

Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12834066

Citation

Giorgi, D, et al. "Isolation and Characterization of S Genome Specific Sequences From Aegilops Sect. Sitopsis Species." Genome, vol. 46, no. 3, 2003, pp. 478-89.
Giorgi D, D'Ovidio R, Tanzarella OA, et al. Isolation and characterization of S genome specific sequences from Aegilops sect. sitopsis species. Genome. 2003;46(3):478-89.
Giorgi, D., D'Ovidio, R., Tanzarella, O. A., Ceoloni, C., & Porceddu, E. (2003). Isolation and characterization of S genome specific sequences from Aegilops sect. sitopsis species. Genome, 46(3), 478-89.
Giorgi D, et al. Isolation and Characterization of S Genome Specific Sequences From Aegilops Sect. Sitopsis Species. Genome. 2003;46(3):478-89. PubMed PMID: 12834066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of S genome specific sequences from Aegilops sect. sitopsis species. AU - Giorgi,D, AU - D'Ovidio,R, AU - Tanzarella,O A, AU - Ceoloni,C, AU - Porceddu,E, PY - 2003/7/2/pubmed PY - 2004/2/5/medline PY - 2003/7/2/entrez SP - 478 EP - 89 JF - Genome JO - Genome VL - 46 IS - 3 N2 - Three S genome specific sequences were isolated from Aegilops sect. sitopsis species using different experimental approaches. Two clones, UTV86 and UTV39, were isolated from a partial genomic library obtained from DNA of Aegilops sharonensis, whereas a third clone, UTV5, was isolated from Aegilops speltoides. The three clones were characterized by sequencing, analysis of methylation, and sequence organization and abundance in some Aegilops and Triticum species. The clones UTV39 and UTV5 belong to the same family of tandem repeated sequences and showed high homology with a sequence already present in nucleotide databases. The UTV86 clone from Ae. sharonensis corresponded to an interspersed low frequency repeated sequence and did not show any significant homology with reported sequences. Southern hybridization experiments, using the cloned sequences as probes, detected polymorphism in the restriction patterns of all the five Aegilops species in section sitopsis. Aegilops speltoides showed the most divergent hybridization pattern. A close relationship was detected between the S genome of Ae. speltoides and the G genome of the wild Triticum timopheevii. In situ hybridization revealed a telomeric and (or) subtelomeric location of the sequences UTV39 and UTV5. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/12834066/Isolation_and_characterization_of_S_genome_specific_sequences_from_Aegilops_sect__sitopsis_species_ L2 - https://cdnsciencepub.com/doi/10.1139/g03-022?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -