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Characterization of six wheat x Thinopyrum intermedium derivatives by GISH, RFLP, and multicolor GISH.
Genome. 2003 Jun; 46(3):490-5.G

Abstract

Restriction fragment length polymorphism (RFLP) analysis and multicolor genomic in situ hybridization (GISH) are useful tools to precisely characterize genetic stocks derived from crosses of wheat (Triticum aestivum) with Thinopyrum intermedium and Thinopyrum elongatum. The wheat x Th. intermedium derived stocks designated Z1, Z2, Z3, Z4, Z5, and Z6 were initially screened by multicolor GISH using Aegilops speltoides genomic DNA for blocking and various combinations of genomic DNA from Th. intermedium, Triticum urartu, and Aegilops tauschii for probes. The probing (GISH) results indicated that lines Z1 and Z3 were alien disomic addition lines with chromosome numbers of 2n = 44. Z2 was a substitution line in which chromosome 2D was substituted by a pair of Th. intermedium chromosomes; this was confirmed by RFLP and muticolour GISH. Z4 (2n = 44) contained two pairs of wheat--Th. intermedium translocated chromosomes; one pair involved A-genome chromosomes, the other involved D- and A- genome chromosomes. Z5 (2n = 44) contained one pair of wheat--Th. intermedium translocated chromosomes involving the D- and A-genome chromosomes of wheat. Z6 (2n = 44) contained one pair of chromosomes derived from Th. intermedium plus another pair of translocated chromosomes involving B-genome chromosomes of wheat Line Z2 was of special interest because it has some resistance to infection by Fusarium graminearum.

Authors+Show Affiliations

Han FP
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, 960 Carling Ave., Bldg 50, Ottawa, ON K1A 0C6, Canada.
Fedak G
No affiliation info available
Benabdelmouna A
No affiliation info available
Armstrong K
No affiliation info available
Ouellet T
No affiliation info available

MeSH

Blotting, SouthernChromosomes, PlantDNA ProbesFusariumHybridization, GeneticIn Situ Hybridization, FluorescencePolymorphism, Restriction Fragment LengthTriticum

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

12834067

Citation

Han, F P., et al. "Characterization of Six Wheat X Thinopyrum Intermedium Derivatives By GISH, RFLP, and Multicolor GISH." Genome, vol. 46, no. 3, 2003, pp. 490-5.
Han FP, Fedak G, Benabdelmouna A, et al. Characterization of six wheat x Thinopyrum intermedium derivatives by GISH, RFLP, and multicolor GISH. Genome. 2003;46(3):490-5.
Han, F. P., Fedak, G., Benabdelmouna, A., Armstrong, K., & Ouellet, T. (2003). Characterization of six wheat x Thinopyrum intermedium derivatives by GISH, RFLP, and multicolor GISH. Genome, 46(3), 490-5.
Han FP, et al. Characterization of Six Wheat X Thinopyrum Intermedium Derivatives By GISH, RFLP, and Multicolor GISH. Genome. 2003;46(3):490-5. PubMed PMID: 12834067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of six wheat x Thinopyrum intermedium derivatives by GISH, RFLP, and multicolor GISH. AU - Han,F P, AU - Fedak,G, AU - Benabdelmouna,A, AU - Armstrong,K, AU - Ouellet,T, PY - 2003/7/2/pubmed PY - 2004/2/5/medline PY - 2003/7/2/entrez SP - 490 EP - 5 JF - Genome JO - Genome VL - 46 IS - 3 N2 - Restriction fragment length polymorphism (RFLP) analysis and multicolor genomic in situ hybridization (GISH) are useful tools to precisely characterize genetic stocks derived from crosses of wheat (Triticum aestivum) with Thinopyrum intermedium and Thinopyrum elongatum. The wheat x Th. intermedium derived stocks designated Z1, Z2, Z3, Z4, Z5, and Z6 were initially screened by multicolor GISH using Aegilops speltoides genomic DNA for blocking and various combinations of genomic DNA from Th. intermedium, Triticum urartu, and Aegilops tauschii for probes. The probing (GISH) results indicated that lines Z1 and Z3 were alien disomic addition lines with chromosome numbers of 2n = 44. Z2 was a substitution line in which chromosome 2D was substituted by a pair of Th. intermedium chromosomes; this was confirmed by RFLP and muticolour GISH. Z4 (2n = 44) contained two pairs of wheat--Th. intermedium translocated chromosomes; one pair involved A-genome chromosomes, the other involved D- and A- genome chromosomes. Z5 (2n = 44) contained one pair of wheat--Th. intermedium translocated chromosomes involving the D- and A-genome chromosomes of wheat. Z6 (2n = 44) contained one pair of chromosomes derived from Th. intermedium plus another pair of translocated chromosomes involving B-genome chromosomes of wheat Line Z2 was of special interest because it has some resistance to infection by Fusarium graminearum. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/12834067/Characterization_of_six_wheat_x_Thinopyrum_intermedium_derivatives_by_GISH_RFLP_and_multicolor_GISH_ DB - PRIME DP - Unbound Medicine ER -