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Ethanol fermentation of acid-hydrolyzed cellulosic pyrolysate with Saccharomyces cerevisiae.
Bioresour Technol. 2003 Oct; 90(1):95-100.BT

Abstract

The acid hydrolysis of cellulosic pyrolysate to glucose and its fermentation to ethanol were investigated. The maximum glucose yield (17.4%) was obtained by the hydrolysis with 0.2 mol sulfuric acid per liter pyrolysate using autoclaving at 121 degrees C for 20 min. The fermentation by Saccharomyces cerevisiae of a hydrolysate medium containing 31.6 g/l glucose gave 14.2 g/l ethanol in 24 h, whereas the fermentation of the medium containing 31.6 g/l pure glucose gave 13.7 g/l ethanol in 18 h. The results showed that the acid-hydrolyzed pyrolysate could be used for ethanol production. Different nitrogen sources were evaluated and the best ethanol concentration (15.1 g/l) was achieved by single urea. S. cerevisiae (R) was obtained by adaptation of S. cerevisiae to the hydrolysate medium for 12 times, and 40.2 g/l ethanol was produced by S. cerevisiae (R) in the fermentation with the hydrolysate medium containing 95.8 g/l glucose, which was about 47% increase in ethanol production compared to its parent strain.

Authors+Show Affiliations

Yu Z
Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, People's Republic of China.
Zhang H
No affiliation info available

MeSH

BioreactorsCellulose, OxidizedCotton FiberEthanolFeasibility StudiesGlucoseHot TemperatureHydrogen-Ion ConcentrationHydrolysisNitrogenPilot ProjectsQuality ControlSaccharomyces cerevisiae

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12835064

Citation

Yu, Zhisheng, and Hongxun Zhang. "Ethanol Fermentation of Acid-hydrolyzed Cellulosic Pyrolysate With Saccharomyces Cerevisiae." Bioresource Technology, vol. 90, no. 1, 2003, pp. 95-100.
Yu Z, Zhang H. Ethanol fermentation of acid-hydrolyzed cellulosic pyrolysate with Saccharomyces cerevisiae. Bioresour Technol. 2003;90(1):95-100.
Yu, Z., & Zhang, H. (2003). Ethanol fermentation of acid-hydrolyzed cellulosic pyrolysate with Saccharomyces cerevisiae. Bioresource Technology, 90(1), 95-100.
Yu Z, Zhang H. Ethanol Fermentation of Acid-hydrolyzed Cellulosic Pyrolysate With Saccharomyces Cerevisiae. Bioresour Technol. 2003;90(1):95-100. PubMed PMID: 12835064.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ethanol fermentation of acid-hydrolyzed cellulosic pyrolysate with Saccharomyces cerevisiae. AU - Yu,Zhisheng, AU - Zhang,Hongxun, PY - 2003/7/2/pubmed PY - 2003/10/31/medline PY - 2003/7/2/entrez SP - 95 EP - 100 JF - Bioresource technology JO - Bioresour Technol VL - 90 IS - 1 N2 - The acid hydrolysis of cellulosic pyrolysate to glucose and its fermentation to ethanol were investigated. The maximum glucose yield (17.4%) was obtained by the hydrolysis with 0.2 mol sulfuric acid per liter pyrolysate using autoclaving at 121 degrees C for 20 min. The fermentation by Saccharomyces cerevisiae of a hydrolysate medium containing 31.6 g/l glucose gave 14.2 g/l ethanol in 24 h, whereas the fermentation of the medium containing 31.6 g/l pure glucose gave 13.7 g/l ethanol in 18 h. The results showed that the acid-hydrolyzed pyrolysate could be used for ethanol production. Different nitrogen sources were evaluated and the best ethanol concentration (15.1 g/l) was achieved by single urea. S. cerevisiae (R) was obtained by adaptation of S. cerevisiae to the hydrolysate medium for 12 times, and 40.2 g/l ethanol was produced by S. cerevisiae (R) in the fermentation with the hydrolysate medium containing 95.8 g/l glucose, which was about 47% increase in ethanol production compared to its parent strain. SN - 0960-8524 UR - https://www.unboundmedicine.com/medline/citation/12835064/Ethanol_fermentation_of_acid_hydrolyzed_cellulosic_pyrolysate_with_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -