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Characterization and formation mechanism of water-insoluble DNA-matrix induced by UV irradiation.

Abstract

We have prepared water-insoluble and nuclease resistant DNA-matrixes by UV irradiation. The UV-irradiated DNA-matrix could effectively accumulate and condense harmful DNA-intercalating compounds, such as acridine orange (AO) and ethidium bromide (EB), from diluted aqueous solutions. The binding constant of AO and EB for UV-irradiated DNA were determined to be 1.0 (+/- 0.2) x 10(5) M-1 and 6.8 (+/- 0.3) x 10(4) M-1, respectively; values consisted with reported results for non-irradiated DNA. In addition, the agarose gel electrophoresis and AFM measurements indicate that DNA matrix forms an intermolecular cross-linking structure with the radical reaction. The UV-irradiated DNA-matrixes have potential uses as a biomaterial filter for the removal of harmful DNA intercalating compounds.

Authors+Show Affiliations

Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12836336

Citation

Yamada, M, et al. "Characterization and Formation Mechanism of Water-insoluble DNA-matrix Induced By UV Irradiation." Nucleic Acids Research. Supplement (2001), 2001, pp. 205-6.
Yamada M, Satoh S, Nomizu M, et al. Characterization and formation mechanism of water-insoluble DNA-matrix induced by UV irradiation. Nucleic Acids Res Suppl. 2001.
Yamada, M., Satoh, S., Nomizu, M., Ohkawa, K., Yamamoto, H., & Nishi, N. (2001). Characterization and formation mechanism of water-insoluble DNA-matrix induced by UV irradiation. Nucleic Acids Research. Supplement (2001), (1), 205-6.
Yamada M, et al. Characterization and Formation Mechanism of Water-insoluble DNA-matrix Induced By UV Irradiation. Nucleic Acids Res Suppl. 2001;(1)205-6. PubMed PMID: 12836336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and formation mechanism of water-insoluble DNA-matrix induced by UV irradiation. AU - Yamada,M, AU - Satoh,S, AU - Nomizu,M, AU - Ohkawa,K, AU - Yamamoto,H, AU - Nishi,N, PY - 2003/7/3/pubmed PY - 2003/8/16/medline PY - 2003/7/3/entrez SP - 205 EP - 6 JF - Nucleic acids research. Supplement (2001) JO - Nucleic Acids Res. Suppl. IS - 1 N2 - We have prepared water-insoluble and nuclease resistant DNA-matrixes by UV irradiation. The UV-irradiated DNA-matrix could effectively accumulate and condense harmful DNA-intercalating compounds, such as acridine orange (AO) and ethidium bromide (EB), from diluted aqueous solutions. The binding constant of AO and EB for UV-irradiated DNA were determined to be 1.0 (+/- 0.2) x 10(5) M-1 and 6.8 (+/- 0.3) x 10(4) M-1, respectively; values consisted with reported results for non-irradiated DNA. In addition, the agarose gel electrophoresis and AFM measurements indicate that DNA matrix forms an intermolecular cross-linking structure with the radical reaction. The UV-irradiated DNA-matrixes have potential uses as a biomaterial filter for the removal of harmful DNA intercalating compounds. UR - https://www.unboundmedicine.com/medline/citation/12836336/Characterization_and_formation_mechanism_of_water_insoluble_DNA_matrix_induced_by_UV_irradiation_ DB - PRIME DP - Unbound Medicine ER -