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[Significance of serum level of NO and IL-8 in Helicobacter pylori associated gastric diseases].
Zhonghua Zhong Liu Za Zhi 2003; 25(3):258-60ZZ

Abstract

OBJECTIVE

To investigate the relation between serum level of interleukin-8 (IL-8), nitrogen monoxide (NO) and Helicobacter pylori (HP) infection, as well as the possible molecular mechanism of HP infection causing the imbalance of apoptosis and proliferation in gastric epithelial cells, which may lead to oncogenesis in stomach.

METHODS

Serum IL-8 level was detected with enzyme-linked immunosorbent assay (ELISA). Serum NO was detected with chrome reduction method using plated copper.

RESULTS

Serum level of IL-8 were 22.50 +/- 1.87 pg/ml in the normal tissue, 34.99 +/- 7.89 pg/ml in superficial gastritis, 65.27 +/- 10.60 pg/ml in atrophic gastritis and 94.84 +/- 11.09 pg/ml in gastric cancer (P < 0.01). Serum level of NO in the atrophic gastritis group (39.93 +/- 5.43 micromol/L) was significantly higher than that in the gastric cancer group (37.02 +/- 4.13 micromol/L) (P < 0.05). The differences in the other groups were not significant. IL-8 and NO levels in the HP(+) group were significantly higher than those in the HP(-) group (77.30 +/- 20.92 pg/ml vs 39.16 +/- 14.40 pg/ml, P < 0.01; 39.77 +/- 5.57 micromol/L vs 35.35 +/- 5.24 micromol/L, P < 0.01). Serum levels of IL-8 and NO in the cytotoxin-associated gene A protein (CagA)(+)HP group were significantly higher than those in the CagA(-)HP group (83.45 +/- 16.92 pg/ml vs 66.24 +/- 23.21 pg/ml, P < 0.01; 40.97 +/- 4.59 micromol/L vs 37.62 +/- 6.58 micromol/L, P < 0.05). Serum levels of IL-8 and NO showed positive correlation between superficial gastritis and atrophic gastritis groups (r = 0.881, r = 0.995), whereas no correlation was found in the normal or gastric cancer groups.

CONCLUSION

Serum levels of IL-8 and NO are correlated with CagA(+)HP strain infection. Combined detection of serum level of IL-8, NO and HP-CagA will contribute to the early diagnosis of precancerous lesion in the stomach.

Authors+Show Affiliations

Cancer Institute, First Hospital, Chinese Medical College, Shenyang 110001, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12839689

Citation

Song, Chun-fang, et al. "[Significance of Serum Level of NO and IL-8 in Helicobacter Pylori Associated Gastric Diseases]." Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], vol. 25, no. 3, 2003, pp. 258-60.
Song CF, Sun LP, Dai WY, et al. [Significance of serum level of NO and IL-8 in Helicobacter pylori associated gastric diseases]. Zhonghua Zhong Liu Za Zhi. 2003;25(3):258-60.
Song, C. F., Sun, L. P., Dai, W. Y., & Yuan, Y. (2003). [Significance of serum level of NO and IL-8 in Helicobacter pylori associated gastric diseases]. Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], 25(3), pp. 258-60.
Song CF, et al. [Significance of Serum Level of NO and IL-8 in Helicobacter Pylori Associated Gastric Diseases]. Zhonghua Zhong Liu Za Zhi. 2003;25(3):258-60. PubMed PMID: 12839689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Significance of serum level of NO and IL-8 in Helicobacter pylori associated gastric diseases]. AU - Song,Chun-fang, AU - Sun,Li-ping, AU - Dai,Wen-ying, AU - Yuan,Yuan, PY - 2003/7/4/pubmed PY - 2010/5/19/medline PY - 2003/7/4/entrez SP - 258 EP - 60 JF - Zhonghua zhong liu za zhi [Chinese journal of oncology] JO - Zhonghua Zhong Liu Za Zhi VL - 25 IS - 3 N2 - OBJECTIVE: To investigate the relation between serum level of interleukin-8 (IL-8), nitrogen monoxide (NO) and Helicobacter pylori (HP) infection, as well as the possible molecular mechanism of HP infection causing the imbalance of apoptosis and proliferation in gastric epithelial cells, which may lead to oncogenesis in stomach. METHODS: Serum IL-8 level was detected with enzyme-linked immunosorbent assay (ELISA). Serum NO was detected with chrome reduction method using plated copper. RESULTS: Serum level of IL-8 were 22.50 +/- 1.87 pg/ml in the normal tissue, 34.99 +/- 7.89 pg/ml in superficial gastritis, 65.27 +/- 10.60 pg/ml in atrophic gastritis and 94.84 +/- 11.09 pg/ml in gastric cancer (P < 0.01). Serum level of NO in the atrophic gastritis group (39.93 +/- 5.43 micromol/L) was significantly higher than that in the gastric cancer group (37.02 +/- 4.13 micromol/L) (P < 0.05). The differences in the other groups were not significant. IL-8 and NO levels in the HP(+) group were significantly higher than those in the HP(-) group (77.30 +/- 20.92 pg/ml vs 39.16 +/- 14.40 pg/ml, P < 0.01; 39.77 +/- 5.57 micromol/L vs 35.35 +/- 5.24 micromol/L, P < 0.01). Serum levels of IL-8 and NO in the cytotoxin-associated gene A protein (CagA)(+)HP group were significantly higher than those in the CagA(-)HP group (83.45 +/- 16.92 pg/ml vs 66.24 +/- 23.21 pg/ml, P < 0.01; 40.97 +/- 4.59 micromol/L vs 37.62 +/- 6.58 micromol/L, P < 0.05). Serum levels of IL-8 and NO showed positive correlation between superficial gastritis and atrophic gastritis groups (r = 0.881, r = 0.995), whereas no correlation was found in the normal or gastric cancer groups. CONCLUSION: Serum levels of IL-8 and NO are correlated with CagA(+)HP strain infection. Combined detection of serum level of IL-8, NO and HP-CagA will contribute to the early diagnosis of precancerous lesion in the stomach. SN - 0253-3766 UR - https://www.unboundmedicine.com/medline/citation/12839689/[Significance_of_serum_level_of_NO_and_IL_8_in_Helicobacter_pylori_associated_gastric_diseases]_ L2 - https://medlineplus.gov/helicobacterpyloriinfections.html DB - PRIME DP - Unbound Medicine ER -