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Temporal relationships between circulating levels of CC and CXC chemokines and developing atherosclerosis in apolipoprotein E*3 Leiden mice.
Arterioscler Thromb Vasc Biol. 2003 Sep 01; 23(9):1615-20.AT

Abstract

OBJECTIVE

CC and CXC chemokines are implicated in leukocyte recruitment during development of atherosclerotic lesions, suggesting circulating levels of chemokines may be useful serum markers of atherogenesis. Serum chemokine concentrations were measured in apolipoprotein (apo) E*3 Leiden mice and their nontransgenic littermates and related to the differing rates of atherogenesis in these animals.

METHODS AND RESULTS

Mice were fed a high-fat, high-cholesterol/cholate (HFC/C) diet for 18 weeks. Circulating levels of JE/monocyte chemotactic protein-1 increased (P<0.05) after 2 to 4 weeks, coincident with development of diet-induced hypercholesterolemia, and remained elevated throughout the study. Circulating KC concentrations increased (P<0.05) after consumption of HFC/C diet; however, unlike JE, serum KC concentrations increased more rapidly in apoE*3 Leiden mice than their nontransgenic littermates. Hepatic expression of JE and KC mRNA were detected by in situ hybridization in all mice fed HFC/C diet. Aortic expression of JE mRNA was seen only in apoE*3 Leiden mice within macrophage-rich atherosclerotic lesions. By contrast, no aortic expression of KC mRNA was detected by in situ hybridization.

CONCLUSIONS

Increases in serum chemokine concentrations did not reflect temporal aortic production of these molecules and proved less predictive than serum cholesterol of the markedly different extent of atheroma in apoE*3 Leiden and nontransgenic mice.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Royal Free and University College Medical School of UCL, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12842836

Citation

Murphy, Nuala, et al. "Temporal Relationships Between Circulating Levels of CC and CXC Chemokines and Developing Atherosclerosis in Apolipoprotein E*3 Leiden Mice." Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 23, no. 9, 2003, pp. 1615-20.
Murphy N, Bruckdorfer KR, Grimsditch DC, et al. Temporal relationships between circulating levels of CC and CXC chemokines and developing atherosclerosis in apolipoprotein E*3 Leiden mice. Arterioscler Thromb Vasc Biol. 2003;23(9):1615-20.
Murphy, N., Bruckdorfer, K. R., Grimsditch, D. C., Overend, P., Vidgeon-Hart, M., Groot, P. H., Benson, G. M., & Graham, A. (2003). Temporal relationships between circulating levels of CC and CXC chemokines and developing atherosclerosis in apolipoprotein E*3 Leiden mice. Arteriosclerosis, Thrombosis, and Vascular Biology, 23(9), 1615-20.
Murphy N, et al. Temporal Relationships Between Circulating Levels of CC and CXC Chemokines and Developing Atherosclerosis in Apolipoprotein E*3 Leiden Mice. Arterioscler Thromb Vasc Biol. 2003 Sep 1;23(9):1615-20. PubMed PMID: 12842836.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporal relationships between circulating levels of CC and CXC chemokines and developing atherosclerosis in apolipoprotein E*3 Leiden mice. AU - Murphy,Nuala, AU - Bruckdorfer,K Richard, AU - Grimsditch,David C, AU - Overend,Philip, AU - Vidgeon-Hart,Martin, AU - Groot,Pieter H E, AU - Benson,G Martin, AU - Graham,Annette, Y1 - 2003/07/03/ PY - 2003/7/5/pubmed PY - 2004/2/5/medline PY - 2003/7/5/entrez SP - 1615 EP - 20 JF - Arteriosclerosis, thrombosis, and vascular biology JO - Arterioscler Thromb Vasc Biol VL - 23 IS - 9 N2 - OBJECTIVE: CC and CXC chemokines are implicated in leukocyte recruitment during development of atherosclerotic lesions, suggesting circulating levels of chemokines may be useful serum markers of atherogenesis. Serum chemokine concentrations were measured in apolipoprotein (apo) E*3 Leiden mice and their nontransgenic littermates and related to the differing rates of atherogenesis in these animals. METHODS AND RESULTS: Mice were fed a high-fat, high-cholesterol/cholate (HFC/C) diet for 18 weeks. Circulating levels of JE/monocyte chemotactic protein-1 increased (P<0.05) after 2 to 4 weeks, coincident with development of diet-induced hypercholesterolemia, and remained elevated throughout the study. Circulating KC concentrations increased (P<0.05) after consumption of HFC/C diet; however, unlike JE, serum KC concentrations increased more rapidly in apoE*3 Leiden mice than their nontransgenic littermates. Hepatic expression of JE and KC mRNA were detected by in situ hybridization in all mice fed HFC/C diet. Aortic expression of JE mRNA was seen only in apoE*3 Leiden mice within macrophage-rich atherosclerotic lesions. By contrast, no aortic expression of KC mRNA was detected by in situ hybridization. CONCLUSIONS: Increases in serum chemokine concentrations did not reflect temporal aortic production of these molecules and proved less predictive than serum cholesterol of the markedly different extent of atheroma in apoE*3 Leiden and nontransgenic mice. SN - 1524-4636 UR - https://www.unboundmedicine.com/medline/citation/12842836/Temporal_relationships_between_circulating_levels_of_CC_and_CXC_chemokines_and_developing_atherosclerosis_in_apolipoprotein_E_3_Leiden_mice_ L2 - https://www.ahajournals.org/doi/10.1161/01.ATV.0000084636.01328.C7?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -