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Acute food deprivation and chronic food restriction differentially affect hypothalamic NPY mRNA expression.
Am J Physiol Regul Integr Comp Physiol. 2003 Nov; 285(5):R1030-6.AJ

Abstract

Although acute food deprivation and chronic food restriction both result in body weight loss, they produce different metabolic states. To evaluate how these two treatments affect hypothalamic peptide systems involved in energy homeostasis, we compared patterns of hypothalamic neuropeptide Y (NPY), agouti-related protein (AgRP), proopiomelanocotin (POMC), and leptin receptor gene expression in acutely food-deprived and chronically food-restricted rats. Both acute food deprivation and chronic food restriction reduced body weight and circulating leptin levels and resulted in increased arcuate NPY and decreased arcuate POMC gene expression. Arcuate AgRP mRNA levels were only elevated in acutely deprived rats. NPY gene expression was increased in the compact subregion of the dorsomedial hypothalamus (DMH) in response to chronic food restriction, but not in response to acute food deprivation. Leptin receptor expression was not affected by either treatment. Double in situ hybridization histochemistry revealed that, in contrast to the situation in the arcuate nucleus, NPY and leptin receptor mRNA-expressing neurons were not colocalized in the DMH. Together, these data suggest that arcuate and DMH NPY gene expression are differentially regulated. DMH NPY-expressing neurons do not appear to be under the direct control of leptin signaling.

Authors+Show Affiliations

Dept. of Psychiatry and Behavioral Sciences, Johns Hopkins Univ. School of Medicine, 720 Rutland Ave., Ross 618, Baltimore, MD 21205, USA. sbi@jhmi.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12842868

Citation

Bi, Sheng, et al. "Acute Food Deprivation and Chronic Food Restriction Differentially Affect Hypothalamic NPY mRNA Expression." American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, vol. 285, no. 5, 2003, pp. R1030-6.
Bi S, Robinson BM, Moran TH. Acute food deprivation and chronic food restriction differentially affect hypothalamic NPY mRNA expression. Am J Physiol Regul Integr Comp Physiol. 2003;285(5):R1030-6.
Bi, S., Robinson, B. M., & Moran, T. H. (2003). Acute food deprivation and chronic food restriction differentially affect hypothalamic NPY mRNA expression. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 285(5), R1030-6.
Bi S, Robinson BM, Moran TH. Acute Food Deprivation and Chronic Food Restriction Differentially Affect Hypothalamic NPY mRNA Expression. Am J Physiol Regul Integr Comp Physiol. 2003;285(5):R1030-6. PubMed PMID: 12842868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute food deprivation and chronic food restriction differentially affect hypothalamic NPY mRNA expression. AU - Bi,Sheng, AU - Robinson,Benjamin M, AU - Moran,Timothy H, Y1 - 2003/07/03/ PY - 2003/7/5/pubmed PY - 2003/12/3/medline PY - 2003/7/5/entrez SP - R1030 EP - 6 JF - American journal of physiology. Regulatory, integrative and comparative physiology JO - Am. J. Physiol. Regul. Integr. Comp. Physiol. VL - 285 IS - 5 N2 - Although acute food deprivation and chronic food restriction both result in body weight loss, they produce different metabolic states. To evaluate how these two treatments affect hypothalamic peptide systems involved in energy homeostasis, we compared patterns of hypothalamic neuropeptide Y (NPY), agouti-related protein (AgRP), proopiomelanocotin (POMC), and leptin receptor gene expression in acutely food-deprived and chronically food-restricted rats. Both acute food deprivation and chronic food restriction reduced body weight and circulating leptin levels and resulted in increased arcuate NPY and decreased arcuate POMC gene expression. Arcuate AgRP mRNA levels were only elevated in acutely deprived rats. NPY gene expression was increased in the compact subregion of the dorsomedial hypothalamus (DMH) in response to chronic food restriction, but not in response to acute food deprivation. Leptin receptor expression was not affected by either treatment. Double in situ hybridization histochemistry revealed that, in contrast to the situation in the arcuate nucleus, NPY and leptin receptor mRNA-expressing neurons were not colocalized in the DMH. Together, these data suggest that arcuate and DMH NPY gene expression are differentially regulated. DMH NPY-expressing neurons do not appear to be under the direct control of leptin signaling. SN - 0363-6119 UR - https://www.unboundmedicine.com/medline/citation/12842868/Acute_food_deprivation_and_chronic_food_restriction_differentially_affect_hypothalamic_NPY_mRNA_expression_ L2 - http://www.physiology.org/doi/full/10.1152/ajpregu.00734.2002?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -