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[Antiviral therapy adjustment in corneal recipients using antibody testing in the aqueous humor].
J Fr Ophtalmol. 2003 Apr; 26(4):350-4.JF

Abstract

INTRODUCTION

In corneal recipients with herpes infection, acyclovir given for 1 year postoperatively prevents viral reactivation and improves graft outcome. The indication for prophylactic antiviral therapy relies on the preoperative diagnosis of herpes. However, many patients present with corneal scars featuring sequelae of herpes without a proven history of herpes. Here we report the results of a prospective study of anti-herpes simplex virus (anti-HSV) and varicella zoster virus (VZV) antibody testing in the aqueous humor at the time of corneal transplantation to refine the indication of the antiviral treatment.

MATERIAL AND METHODS

The study involved 33 keratitis corneal graft recipients, 21 of whom had documented herpes keratitis. A control group was made with 11 cataract patients. An anterior chamber puncture was performed just before surgery. The micro-ELISA test was done on both aqueous humor and serum, and local anti-HSV or VZV antibody synthesis was acknowledged if the ratio of antibody concentrations was above 4.

RESULTS

Local antibody synthesis to HSV was detected in 22 cases, to VZV in 9 cases, to both HSV and VZV in 6 cases, and no synthesis in 8 cases. The sensitivity of the test was 65% in patients with a documented history of herpes (14 cases out of 21). Among non-herpetic patients, the test was positive in 9 patients, who thus benefited from postoperative antiviral therapy. No viral reactivation was encountered after a minimum follow-up of 1 year.

CONCLUSIONS

Antibody testing in the aqueous humor at the time of keratoplasty is a convenient, inexpensive diagnostic tool in corneal recipients. It provides useful information before prescribing a long and expensive postoperative antiviral therapy.

Authors+Show Affiliations

Service d'Ophtalmologie, CHU Dupuytren, Limoges, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

fre

PubMed ID

12843891

Citation

Robert, P-Y, et al. "[Antiviral Therapy Adjustment in Corneal Recipients Using Antibody Testing in the Aqueous Humor]." Journal Francais D'ophtalmologie, vol. 26, no. 4, 2003, pp. 350-4.
Robert PY, Liekfeld A, Ranger-Rogez S, et al. [Antiviral therapy adjustment in corneal recipients using antibody testing in the aqueous humor]. J Fr Ophtalmol. 2003;26(4):350-4.
Robert, P. Y., Liekfeld, A., Ranger-Rogez, S., Jaekel, C., Pleyer, U., Adenis, J. P., & Hartmann, C. (2003). [Antiviral therapy adjustment in corneal recipients using antibody testing in the aqueous humor]. Journal Francais D'ophtalmologie, 26(4), 350-4.
Robert PY, et al. [Antiviral Therapy Adjustment in Corneal Recipients Using Antibody Testing in the Aqueous Humor]. J Fr Ophtalmol. 2003;26(4):350-4. PubMed PMID: 12843891.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Antiviral therapy adjustment in corneal recipients using antibody testing in the aqueous humor]. AU - Robert,P-Y, AU - Liekfeld,A, AU - Ranger-Rogez,S, AU - Jaekel,C, AU - Pleyer,U, AU - Adenis,J-P, AU - Hartmann,C, PY - 2003/7/5/pubmed PY - 2003/8/21/medline PY - 2003/7/5/entrez SP - 350 EP - 4 JF - Journal francais d'ophtalmologie JO - J Fr Ophtalmol VL - 26 IS - 4 N2 - INTRODUCTION: In corneal recipients with herpes infection, acyclovir given for 1 year postoperatively prevents viral reactivation and improves graft outcome. The indication for prophylactic antiviral therapy relies on the preoperative diagnosis of herpes. However, many patients present with corneal scars featuring sequelae of herpes without a proven history of herpes. Here we report the results of a prospective study of anti-herpes simplex virus (anti-HSV) and varicella zoster virus (VZV) antibody testing in the aqueous humor at the time of corneal transplantation to refine the indication of the antiviral treatment. MATERIAL AND METHODS: The study involved 33 keratitis corneal graft recipients, 21 of whom had documented herpes keratitis. A control group was made with 11 cataract patients. An anterior chamber puncture was performed just before surgery. The micro-ELISA test was done on both aqueous humor and serum, and local anti-HSV or VZV antibody synthesis was acknowledged if the ratio of antibody concentrations was above 4. RESULTS: Local antibody synthesis to HSV was detected in 22 cases, to VZV in 9 cases, to both HSV and VZV in 6 cases, and no synthesis in 8 cases. The sensitivity of the test was 65% in patients with a documented history of herpes (14 cases out of 21). Among non-herpetic patients, the test was positive in 9 patients, who thus benefited from postoperative antiviral therapy. No viral reactivation was encountered after a minimum follow-up of 1 year. CONCLUSIONS: Antibody testing in the aqueous humor at the time of keratoplasty is a convenient, inexpensive diagnostic tool in corneal recipients. It provides useful information before prescribing a long and expensive postoperative antiviral therapy. SN - 0181-5512 UR - https://www.unboundmedicine.com/medline/citation/12843891/[Antiviral_therapy_adjustment_in_corneal_recipients_using_antibody_testing_in_the_aqueous_humor]_ L2 - https://medlineplus.gov/aftersurgery.html DB - PRIME DP - Unbound Medicine ER -