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Laboratory and field experiments used to identify Canis lupus var. familiaris active odor signature chemicals from drugs, explosives, and humans.
Anal Bioanal Chem. 2003 Aug; 376(8):1212-24.AB

Abstract

This paper describes the use of headspace solid-phase microextraction (SPME) combined with gas chromatography to identify the signature odors that law enforcement-certified detector dogs alert to when searching for drugs, explosives, and humans. Background information is provided on the many types of detector dog available and specific samples highlighted in this paper are the drugs cocaine and 3,4-methylenedioxy- N-methylamphetamine (MDMA or Ecstasy), the explosives TNT and C4, and human remains. Studies include the analysis and identification of the headspace "fingerprint" of a variety of samples, followed by completion of double-blind dog trials of the individual components in an attempt to isolate and understand the target compounds that dogs alert to. SPME-GC/MS has been demonstrated to have a unique capability for the extraction of volatiles from the headspace of forensic specimens including drugs and explosives and shows great potential to aid in the investigation and understanding of the complicated process of canine odor detection. Major variables evaluated for the headspace SPME included fiber chemistry and a variety of sampling times ranging from several hours to several seconds and the resultant effect on ratios of isolated volatile components. For the drug odor studies, the CW/DVB and PDMS SPME fibers proved to be the optimal fiber types. For explosives, the results demonstrated that the best fibers in field and laboratory applications were PDMS and CW/DVB, respectively. Gas chromatography with electron capture detector (GC/ECD) and mass spectrometry (GC/MS) was better for analysis of nitromethane and TNT odors, and C-4 odors, respectively. Field studies with detector dogs have demonstrated possible candidates for new pseudo scents as well as the potential use of controlled permeation devices as non-hazardous training aids providing consistent permeation of target odors.

Authors+Show Affiliations

Department of Chemistry and International Forensic Research Institute (IFRI), Florida International University, University Park, Miami, FL 33199, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12845400

Citation

Lorenzo, Norma, et al. "Laboratory and Field Experiments Used to Identify Canis Lupus Var. Familiaris Active Odor Signature Chemicals From Drugs, Explosives, and Humans." Analytical and Bioanalytical Chemistry, vol. 376, no. 8, 2003, pp. 1212-24.
Lorenzo N, Wan T, Harper RJ, et al. Laboratory and field experiments used to identify Canis lupus var. familiaris active odor signature chemicals from drugs, explosives, and humans. Anal Bioanal Chem. 2003;376(8):1212-24.
Lorenzo, N., Wan, T., Harper, R. J., Hsu, Y. L., Chow, M., Rose, S., & Furton, K. G. (2003). Laboratory and field experiments used to identify Canis lupus var. familiaris active odor signature chemicals from drugs, explosives, and humans. Analytical and Bioanalytical Chemistry, 376(8), 1212-24.
Lorenzo N, et al. Laboratory and Field Experiments Used to Identify Canis Lupus Var. Familiaris Active Odor Signature Chemicals From Drugs, Explosives, and Humans. Anal Bioanal Chem. 2003;376(8):1212-24. PubMed PMID: 12845400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Laboratory and field experiments used to identify Canis lupus var. familiaris active odor signature chemicals from drugs, explosives, and humans. AU - Lorenzo,Norma, AU - Wan,TianLang, AU - Harper,Ross J, AU - Hsu,Ya-Li, AU - Chow,Michael, AU - Rose,Stefan, AU - Furton,Kenneth G, Y1 - 2003/07/04/ PY - 2003/01/31/received PY - 2003/04/28/revised PY - 2003/04/29/accepted PY - 2003/7/8/pubmed PY - 2003/10/16/medline PY - 2003/7/8/entrez SP - 1212 EP - 24 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 376 IS - 8 N2 - This paper describes the use of headspace solid-phase microextraction (SPME) combined with gas chromatography to identify the signature odors that law enforcement-certified detector dogs alert to when searching for drugs, explosives, and humans. Background information is provided on the many types of detector dog available and specific samples highlighted in this paper are the drugs cocaine and 3,4-methylenedioxy- N-methylamphetamine (MDMA or Ecstasy), the explosives TNT and C4, and human remains. Studies include the analysis and identification of the headspace "fingerprint" of a variety of samples, followed by completion of double-blind dog trials of the individual components in an attempt to isolate and understand the target compounds that dogs alert to. SPME-GC/MS has been demonstrated to have a unique capability for the extraction of volatiles from the headspace of forensic specimens including drugs and explosives and shows great potential to aid in the investigation and understanding of the complicated process of canine odor detection. Major variables evaluated for the headspace SPME included fiber chemistry and a variety of sampling times ranging from several hours to several seconds and the resultant effect on ratios of isolated volatile components. For the drug odor studies, the CW/DVB and PDMS SPME fibers proved to be the optimal fiber types. For explosives, the results demonstrated that the best fibers in field and laboratory applications were PDMS and CW/DVB, respectively. Gas chromatography with electron capture detector (GC/ECD) and mass spectrometry (GC/MS) was better for analysis of nitromethane and TNT odors, and C-4 odors, respectively. Field studies with detector dogs have demonstrated possible candidates for new pseudo scents as well as the potential use of controlled permeation devices as non-hazardous training aids providing consistent permeation of target odors. SN - 1618-2642 UR - https://www.unboundmedicine.com/medline/citation/12845400/Laboratory_and_field_experiments_used_to_identify_Canis_lupus_var__familiaris_active_odor_signature_chemicals_from_drugs_explosives_and_humans_ L2 - https://dx.doi.org/10.1007/s00216-003-2018-7 DB - PRIME DP - Unbound Medicine ER -