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Urine drug testing for opioids, cocaine, and metabolites by direct injection liquid chromatography/tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2003; 17(14):1665-70.RC

Abstract

A sensitive and specific liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the simultaneous quantification of opioids, cocaine, and metabolites in urine was developed and validated. A 10-microL aliquot of urine was injected directly onto the LC/MS/MS system. The lack of sample preparation substantially reduced total analysis time. Separation was performed by reversed-phase chromatography with gradient elution for all analytes in 26 min. Atmospheric pressure chemical ionization (APCI) was a rugged and efficient ionization technique for basic drugs. Identification and quantification was based on selected reaction monitoring (SRM). Calibration, with deuterated internal standards, was performed by linear regression analysis (weighting factor 1/x). Limits of quantitation (LOQ) were established between 10-100 ng/mL and linearity was obtained up to a maximum of 10 000 ng/mL with an average correlation coefficient (R(2)) > 0.99. Analytical validation criteria for specificity, precision, accuracy, dilution integrity, matrix effect, and stability were fulfilled. The method proved to be simple and time efficient, and was applicable for illicit drug use monitoring and methadone treatment compliance in clinical research projects at the National Institute on Drug Abuse (NIDA).

Authors+Show Affiliations

Chemistry and Drug Metabolism, National Institute on Drug Abuse, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12845594

Citation

Dams, Riet, et al. "Urine Drug Testing for Opioids, Cocaine, and Metabolites By Direct Injection Liquid Chromatography/tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 17, no. 14, 2003, pp. 1665-70.
Dams R, Murphy CM, Lambert WE, et al. Urine drug testing for opioids, cocaine, and metabolites by direct injection liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom. 2003;17(14):1665-70.
Dams, R., Murphy, C. M., Lambert, W. E., & Huestis, M. A. (2003). Urine drug testing for opioids, cocaine, and metabolites by direct injection liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 17(14), 1665-70.
Dams R, et al. Urine Drug Testing for Opioids, Cocaine, and Metabolites By Direct Injection Liquid Chromatography/tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2003;17(14):1665-70. PubMed PMID: 12845594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Urine drug testing for opioids, cocaine, and metabolites by direct injection liquid chromatography/tandem mass spectrometry. AU - Dams,Riet, AU - Murphy,Constance M, AU - Lambert,Willy E, AU - Huestis,Marilyn A, PY - 2003/7/8/pubmed PY - 2003/8/20/medline PY - 2003/7/8/entrez SP - 1665 EP - 70 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 17 IS - 14 N2 - A sensitive and specific liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the simultaneous quantification of opioids, cocaine, and metabolites in urine was developed and validated. A 10-microL aliquot of urine was injected directly onto the LC/MS/MS system. The lack of sample preparation substantially reduced total analysis time. Separation was performed by reversed-phase chromatography with gradient elution for all analytes in 26 min. Atmospheric pressure chemical ionization (APCI) was a rugged and efficient ionization technique for basic drugs. Identification and quantification was based on selected reaction monitoring (SRM). Calibration, with deuterated internal standards, was performed by linear regression analysis (weighting factor 1/x). Limits of quantitation (LOQ) were established between 10-100 ng/mL and linearity was obtained up to a maximum of 10 000 ng/mL with an average correlation coefficient (R(2)) > 0.99. Analytical validation criteria for specificity, precision, accuracy, dilution integrity, matrix effect, and stability were fulfilled. The method proved to be simple and time efficient, and was applicable for illicit drug use monitoring and methadone treatment compliance in clinical research projects at the National Institute on Drug Abuse (NIDA). SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/12845594/Urine_drug_testing_for_opioids_cocaine_and_metabolites_by_direct_injection_liquid_chromatography/tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.1098 DB - PRIME DP - Unbound Medicine ER -