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Ethylene and jasmonic acid signaling affect the NPR1-independent expression of defense genes without impacting resistance to Pseudomonas syringae and Peronospora parasitica in the Arabidopsis ssi1 mutant.
Mol Plant Microbe Interact. 2003 Jul; 16(7):588-99.MP

Abstract

Salicylic acid (SA), ethylene, and jasmonic acid (JA) are important signaling molecules in plant defense to biotic stress. An intricate signaling network involving SA, ethylene, and JA fine tunes plant defense responses. SA-dependent defense responses in Arabidopsis thaliana are mediated through NPR1-dependent and -independent mechanisms. We have previously shown that activation of an NPR1-independent defense mechanism confers enhanced disease resistance and constitutive expression of the pathogenesis-related (PR) genes in the Arabidopsis ssi1 mutant. In addition, the ssi1 mutant constitutively expresses the defensin gene PDF1.2. Moreover, SA is required for the ssi1-conferred constitutive expression of PDF1.2 in addition to PR genes. Hence, the ssi1 mutant appears to target a step common to SA- and ethylene- or JA-regulated defense pathways. In the present study, we show that, in addition to SA, ethylene and JA signaling also are required for the ssi1-conferred constitutive expression of PDF1.2 and the NPR1-independent expression of PR-1. Furthermore, the ethylene-insensitive ein2 and JA-insensitive jar1 mutants enhance susceptibility of ssi1 plants to the necrotrophic fungus Botrytis cinerea. However, defects in either the ethylene- or JA-signaling pathways do not compromise ssi1-conferred resistance to the bacterial pathogen Pseudomonas synringae pv. maculicola and the oomycete pathogen Peronospora parasitica. Interestingly, ssi1 exhibits a marginal increase in the levels of ethylene and JA, suggesting that low endogenous levels of these phytohormones are sufficient to activate expression of defense genes. Taken together, our results indicate that although cross talk in ssi1 renders expression of ethylene- or JA-responsive defense genes sensitive to SA and vice versa, it does not affect downstream signaling leading to resistance.

Authors+Show Affiliations

Division of Biology, Kansas State University, Manhattan 66506, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12848424

Citation

Nandi, Ashis, et al. "Ethylene and Jasmonic Acid Signaling Affect the NPR1-independent Expression of Defense Genes Without Impacting Resistance to Pseudomonas Syringae and Peronospora Parasitica in the Arabidopsis Ssi1 Mutant." Molecular Plant-microbe Interactions : MPMI, vol. 16, no. 7, 2003, pp. 588-99.
Nandi A, Kachroo P, Fukushige H, et al. Ethylene and jasmonic acid signaling affect the NPR1-independent expression of defense genes without impacting resistance to Pseudomonas syringae and Peronospora parasitica in the Arabidopsis ssi1 mutant. Mol Plant Microbe Interact. 2003;16(7):588-99.
Nandi, A., Kachroo, P., Fukushige, H., Hildebrand, D. F., Klessig, D. F., & Shah, J. (2003). Ethylene and jasmonic acid signaling affect the NPR1-independent expression of defense genes without impacting resistance to Pseudomonas syringae and Peronospora parasitica in the Arabidopsis ssi1 mutant. Molecular Plant-microbe Interactions : MPMI, 16(7), 588-99.
Nandi A, et al. Ethylene and Jasmonic Acid Signaling Affect the NPR1-independent Expression of Defense Genes Without Impacting Resistance to Pseudomonas Syringae and Peronospora Parasitica in the Arabidopsis Ssi1 Mutant. Mol Plant Microbe Interact. 2003;16(7):588-99. PubMed PMID: 12848424.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ethylene and jasmonic acid signaling affect the NPR1-independent expression of defense genes without impacting resistance to Pseudomonas syringae and Peronospora parasitica in the Arabidopsis ssi1 mutant. AU - Nandi,Ashis, AU - Kachroo,Pradeep, AU - Fukushige,Hirotada, AU - Hildebrand,David F, AU - Klessig,Daniel F, AU - Shah,Jyoti, PY - 2003/7/10/pubmed PY - 2003/9/18/medline PY - 2003/7/10/entrez SP - 588 EP - 99 JF - Molecular plant-microbe interactions : MPMI JO - Mol. Plant Microbe Interact. VL - 16 IS - 7 N2 - Salicylic acid (SA), ethylene, and jasmonic acid (JA) are important signaling molecules in plant defense to biotic stress. An intricate signaling network involving SA, ethylene, and JA fine tunes plant defense responses. SA-dependent defense responses in Arabidopsis thaliana are mediated through NPR1-dependent and -independent mechanisms. We have previously shown that activation of an NPR1-independent defense mechanism confers enhanced disease resistance and constitutive expression of the pathogenesis-related (PR) genes in the Arabidopsis ssi1 mutant. In addition, the ssi1 mutant constitutively expresses the defensin gene PDF1.2. Moreover, SA is required for the ssi1-conferred constitutive expression of PDF1.2 in addition to PR genes. Hence, the ssi1 mutant appears to target a step common to SA- and ethylene- or JA-regulated defense pathways. In the present study, we show that, in addition to SA, ethylene and JA signaling also are required for the ssi1-conferred constitutive expression of PDF1.2 and the NPR1-independent expression of PR-1. Furthermore, the ethylene-insensitive ein2 and JA-insensitive jar1 mutants enhance susceptibility of ssi1 plants to the necrotrophic fungus Botrytis cinerea. However, defects in either the ethylene- or JA-signaling pathways do not compromise ssi1-conferred resistance to the bacterial pathogen Pseudomonas synringae pv. maculicola and the oomycete pathogen Peronospora parasitica. Interestingly, ssi1 exhibits a marginal increase in the levels of ethylene and JA, suggesting that low endogenous levels of these phytohormones are sufficient to activate expression of defense genes. Taken together, our results indicate that although cross talk in ssi1 renders expression of ethylene- or JA-responsive defense genes sensitive to SA and vice versa, it does not affect downstream signaling leading to resistance. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/12848424/Ethylene_and_jasmonic_acid_signaling_affect_the_NPR1_independent_expression_of_defense_genes_without_impacting_resistance_to_Pseudomonas_syringae_and_Peronospora_parasitica_in_the_Arabidopsis_ssi1_mutant_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/MPMI.2003.16.7.588?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -