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[The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome].
Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Jun; 24(6):484-6.ZL

Abstract

OBJECTIVE

To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS.

METHODS

Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire.

RESULTS

The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM. Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (chi(2) for trend = 16.376, P = 0.00005 for IgG; chi(2) for trend = 28.736, P = 0.00000 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%.

CONCLUSION

IFA can be used to assist diagnosis of SARS after 10 days of the onset of disease.

Authors+Show Affiliations

Fang LQ
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Zhang PH
No affiliation info available
Yang BA
No affiliation info available
Wu XM
No affiliation info available
Zhao QM
No affiliation info available
Liu W
No affiliation info available
Liu H
No affiliation info available
Deng YQ
No affiliation info available
Zhan L
No affiliation info available
Han WG
No affiliation info available
Lu FS
No affiliation info available
Wu JS
No affiliation info available
Yang H
No affiliation info available
Zhu QY
No affiliation info available
Cao WC
No affiliation info available

MeSH

Antibodies, ViralEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectHumansImmunoglobulin GImmunoglobulin MSevere acute respiratory syndrome-related coronavirusSevere Acute Respiratory Syndrome

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12848915

Citation

Fang, Li-qun, et al. "[The Application of Indirect Immuno-fluorescence Assay in the Diagnosis of Severe Acute Respiratory Syndrome]." Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, vol. 24, no. 6, 2003, pp. 484-6.
Fang LQ, Zhang PH, Yang BA, et al. [The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome]. Zhonghua Liu Xing Bing Xue Za Zhi. 2003;24(6):484-6.
Fang, L. Q., Zhang, P. H., Yang, B. A., Wu, X. M., Zhao, Q. M., Liu, W., Liu, H., Deng, Y. Q., Zhan, L., Han, W. G., Lu, F. S., Wu, J. S., Yang, H., Zhu, Q. Y., & Cao, W. C. (2003). [The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome]. Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 24(6), 484-6.
Fang LQ, et al. [The Application of Indirect Immuno-fluorescence Assay in the Diagnosis of Severe Acute Respiratory Syndrome]. Zhonghua Liu Xing Bing Xue Za Zhi. 2003;24(6):484-6. PubMed PMID: 12848915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome]. AU - Fang,Li-qun, AU - Zhang,Pan-he, AU - Yang,Bao-an, AU - Wu,Xiao-ming, AU - Zhao,Qiu-min, AU - Liu,Wei, AU - Liu,Hong, AU - Deng,Yong-qiang, AU - Zhan,Lin, AU - Han,Wei-guo, AU - Lu,Fu-shuang, AU - Wu,Jin-song, AU - Yang,Hong, AU - Zhu,Qing-yu, AU - Cao,Wu-chun, PY - 2003/7/10/pubmed PY - 2003/12/19/medline PY - 2003/7/10/entrez SP - 484 EP - 6 JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi JO - Zhonghua Liu Xing Bing Xue Za Zhi VL - 24 IS - 6 N2 - OBJECTIVE: To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS. METHODS: Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire. RESULTS: The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM. Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (chi(2) for trend = 16.376, P = 0.00005 for IgG; chi(2) for trend = 28.736, P = 0.00000 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%. CONCLUSION: IFA can be used to assist diagnosis of SARS after 10 days of the onset of disease. SN - 0254-6450 UR - https://www.unboundmedicine.com/medline/citation/12848915/[The_application_of_indirect_immuno_fluorescence_assay_in_the_diagnosis_of_severe_acute_respiratory_syndrome]_ DB - PRIME DP - Unbound Medicine ER -