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Analysis of gastrinomas by immunohistochemistry and in situ hybridization histochemistry.
Diagn Mol Pathol. 1992 Sep; 1(3):155-64.DM

Abstract

Gastrinomas from 25 patients were examined by immunohistochemistry (IHC) and in situ hybridization histochemistry (ISH). Most patients (84%) presented with the Zollinger-Ellison syndrome. Six had multiple endocrine neoplasia type I (MEN-I). Twelve patients (48%) had duodenal primaries and 11 of 12 of these had metastases to regional lymph nodes and/or liver in spite of the small sizes of the primary tumors (mean size of 0.9 cm). Five patients had pancreatic gastrinomas and eight patients had metastatic tumor in regional lymph nodes or liver at surgery but a primary was not found. IHC and ISH analyses showed that all cases were positive for gastrin protein and 24 of 25 (96%) expressed gastrin mRNA that was easily detected in formalin-fixed, paraffin-embedded tissue sections. Both benign and malignant tumors expressed alpha subunit of human chorionic gonadotropin protein (alpha-HCG). However, only malignant gastrinomas (29%) expressed adrenocorticotropic hormone protein or proopiomelanocortin (POMC) mRNA. ISH and Northern hybridization analysis revealed that chromogranin A mRNA was the most common member of the chromogranin/secretogranin (Cg/Sg) family which was expressed in both benign and malignant gastrinomas. These results indicate that duodenal gastrinomas are common in both sporadic and MEN-1-associated cases, and small duodenal primaries may be associated with extensive regional lymph node and liver metastases. Expression of ACTH/POMC protein and mRNA was consistently associated only with malignant gastrinomas while gastrin protein, gastrin mRNA and Cgs/Sgs mRNAs were readily detected in both benign and malignant gastrinomas.

Authors+Show Affiliations

Department of Pathology, University of Michigan Medical Center, Ann Arbor 48109-0054.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1285276

Citation

Perkins, P L., et al. "Analysis of Gastrinomas By Immunohistochemistry and in Situ Hybridization Histochemistry." Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B, vol. 1, no. 3, 1992, pp. 155-64.
Perkins PL, McLeod MK, Jin L, et al. Analysis of gastrinomas by immunohistochemistry and in situ hybridization histochemistry. Diagn Mol Pathol. 1992;1(3):155-64.
Perkins, P. L., McLeod, M. K., Jin, L., Fukuuchi, A., Cho, K. J., Thompson, N. W., & Lloyd, R. V. (1992). Analysis of gastrinomas by immunohistochemistry and in situ hybridization histochemistry. Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B, 1(3), 155-64.
Perkins PL, et al. Analysis of Gastrinomas By Immunohistochemistry and in Situ Hybridization Histochemistry. Diagn Mol Pathol. 1992;1(3):155-64. PubMed PMID: 1285276.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of gastrinomas by immunohistochemistry and in situ hybridization histochemistry. AU - Perkins,P L, AU - McLeod,M K, AU - Jin,L, AU - Fukuuchi,A, AU - Cho,K J, AU - Thompson,N W, AU - Lloyd,R V, PY - 1992/9/1/pubmed PY - 1992/9/1/medline PY - 1992/9/1/entrez SP - 155 EP - 64 JF - Diagnostic molecular pathology : the American journal of surgical pathology, part B JO - Diagn Mol Pathol VL - 1 IS - 3 N2 - Gastrinomas from 25 patients were examined by immunohistochemistry (IHC) and in situ hybridization histochemistry (ISH). Most patients (84%) presented with the Zollinger-Ellison syndrome. Six had multiple endocrine neoplasia type I (MEN-I). Twelve patients (48%) had duodenal primaries and 11 of 12 of these had metastases to regional lymph nodes and/or liver in spite of the small sizes of the primary tumors (mean size of 0.9 cm). Five patients had pancreatic gastrinomas and eight patients had metastatic tumor in regional lymph nodes or liver at surgery but a primary was not found. IHC and ISH analyses showed that all cases were positive for gastrin protein and 24 of 25 (96%) expressed gastrin mRNA that was easily detected in formalin-fixed, paraffin-embedded tissue sections. Both benign and malignant tumors expressed alpha subunit of human chorionic gonadotropin protein (alpha-HCG). However, only malignant gastrinomas (29%) expressed adrenocorticotropic hormone protein or proopiomelanocortin (POMC) mRNA. ISH and Northern hybridization analysis revealed that chromogranin A mRNA was the most common member of the chromogranin/secretogranin (Cg/Sg) family which was expressed in both benign and malignant gastrinomas. These results indicate that duodenal gastrinomas are common in both sporadic and MEN-1-associated cases, and small duodenal primaries may be associated with extensive regional lymph node and liver metastases. Expression of ACTH/POMC protein and mRNA was consistently associated only with malignant gastrinomas while gastrin protein, gastrin mRNA and Cgs/Sgs mRNAs were readily detected in both benign and malignant gastrinomas. SN - 1052-9551 UR - https://www.unboundmedicine.com/medline/citation/1285276/Analysis_of_gastrinomas_by_immunohistochemistry_and_in_situ_hybridization_histochemistry_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=1285276.ui DB - PRIME DP - Unbound Medicine ER -