Tags

Type your tag names separated by a space and hit enter

Hippocampal cGMP-dependent protein kinase I supports an age- and protein synthesis-dependent component of long-term potentiation but is not essential for spatial reference and contextual memory.
J Neurosci 2003; 23(14):6005-12JN

Abstract

cGMP-dependent protein kinase I (cGKI) is expressed in the hippocampus, but its role in hippocampal long-term potentiation (LTP) is controversial. In addition, whether cGKI is involved in spatial learning has not been investigated. To address these issues, we generated mice with a hippocampus-specific deletion of cGKI (cGKIhko mice). Unlike conventional cGKI knock-out mice, cGKIhko mice lack gastrointestinal and cardiovascular phenotypes and have a normal life expectancy, which enables us to analyze hippocampal synaptic plasticity and learning in young and adult animals. Hippocampal LTP after repetitive episodes of theta burst stimulation was impaired in adult (12-14 weeks of age) but not in juvenile (3-4 weeks of age) cGKIhko mice. The difference in LTP between adult control and cGKIhko mice was abolished by the protein synthesis inhibitor anisomycin, suggesting that the impairment of LTP in adult cGKIhko mice reflects a defect in late-phase LTP. Despite their deficit in LTP, adult cGKIhko mutants showed normal performance in a discriminatory water maze and had intact contextual fear conditioning. These results suggest that hippocampal cGKI supports an age- and protein synthesis-dependent form of hippocampal LTP, whereas it is dispensable for hippocampus-dependent spatial reference and contextual memory.

Authors+Show Affiliations

Institut für Pharmakologie und Toxikologie, 80802 München, Germany, Max-Planck-Institut für Psychiatrie, 80804 München, Germany. kleppisch@ipt.med.tu-muenchen.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12853418

Citation

Kleppisch, Thomas, et al. "Hippocampal cGMP-dependent Protein Kinase I Supports an Age- and Protein Synthesis-dependent Component of Long-term Potentiation but Is Not Essential for Spatial Reference and Contextual Memory." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 23, no. 14, 2003, pp. 6005-12.
Kleppisch T, Wolfsgruber W, Feil S, et al. Hippocampal cGMP-dependent protein kinase I supports an age- and protein synthesis-dependent component of long-term potentiation but is not essential for spatial reference and contextual memory. J Neurosci. 2003;23(14):6005-12.
Kleppisch, T., Wolfsgruber, W., Feil, S., Allmann, R., Wotjak, C. T., Goebbels, S., ... Feil, R. (2003). Hippocampal cGMP-dependent protein kinase I supports an age- and protein synthesis-dependent component of long-term potentiation but is not essential for spatial reference and contextual memory. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 23(14), pp. 6005-12.
Kleppisch T, et al. Hippocampal cGMP-dependent Protein Kinase I Supports an Age- and Protein Synthesis-dependent Component of Long-term Potentiation but Is Not Essential for Spatial Reference and Contextual Memory. J Neurosci. 2003 Jul 9;23(14):6005-12. PubMed PMID: 12853418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hippocampal cGMP-dependent protein kinase I supports an age- and protein synthesis-dependent component of long-term potentiation but is not essential for spatial reference and contextual memory. AU - Kleppisch,Thomas, AU - Wolfsgruber,Wiebke, AU - Feil,Susanne, AU - Allmann,Rüdiger, AU - Wotjak,Carsten T, AU - Goebbels,Sandra, AU - Nave,Klaus-Armin, AU - Hofmann,Franz, AU - Feil,Robert, PY - 2003/7/11/pubmed PY - 2003/8/9/medline PY - 2003/7/11/entrez SP - 6005 EP - 12 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J. Neurosci. VL - 23 IS - 14 N2 - cGMP-dependent protein kinase I (cGKI) is expressed in the hippocampus, but its role in hippocampal long-term potentiation (LTP) is controversial. In addition, whether cGKI is involved in spatial learning has not been investigated. To address these issues, we generated mice with a hippocampus-specific deletion of cGKI (cGKIhko mice). Unlike conventional cGKI knock-out mice, cGKIhko mice lack gastrointestinal and cardiovascular phenotypes and have a normal life expectancy, which enables us to analyze hippocampal synaptic plasticity and learning in young and adult animals. Hippocampal LTP after repetitive episodes of theta burst stimulation was impaired in adult (12-14 weeks of age) but not in juvenile (3-4 weeks of age) cGKIhko mice. The difference in LTP between adult control and cGKIhko mice was abolished by the protein synthesis inhibitor anisomycin, suggesting that the impairment of LTP in adult cGKIhko mice reflects a defect in late-phase LTP. Despite their deficit in LTP, adult cGKIhko mutants showed normal performance in a discriminatory water maze and had intact contextual fear conditioning. These results suggest that hippocampal cGKI supports an age- and protein synthesis-dependent form of hippocampal LTP, whereas it is dispensable for hippocampus-dependent spatial reference and contextual memory. SN - 1529-2401 UR - https://www.unboundmedicine.com/medline/citation/12853418/Hippocampal_cGMP_dependent_protein_kinase_I_supports_an_age__and_protein_synthesis_dependent_component_of_long_term_potentiation_but_is_not_essential_for_spatial_reference_and_contextual_memory_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=12853418 DB - PRIME DP - Unbound Medicine ER -