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Altered gene expression profile in the kidney of vitamin D receptor knockout mice.
J Cell Biochem 2003; 89(4):709-19JC

Abstract

The kidney is a primary target organ of the vitamin D endocrine system, and both vitamin D-deficiency and vitamin D receptor (VDR) ablation lead to impaired renal functions. As an initial step to understand the molecular basis underlying the renal dysfunctions resulted from VDR inactivation, we used DNA microarray technology to search for changes in the gene expression profile in the kidney of VDR knockout mice. Three independent DNA microarray experiments were performed using Affymetrix GeneChips, which included two replicate comparisons between VDR null and wild-type littermates, and a third comparison between 1,25-dihydroxyvitamin D(3)-treated and vehicle-treated wild-type mice. Based on the assumption that VDR inactivation and vitamin D stimulation cause opposite changes in the expression of vitamin D target genes, we identified 95 genes that displayed the same changes in the two VDR-null/wild-type comparisons but an opposite change in the third assay, of which 28 genes were up-regulated and 67 were down-regulated in VDR null mice. These genes can be divided into several functional categories involved in vitamin D and steroid metabolism, calcium metabolism and signaling, volume and electrolyte homeostasis, signal transduction, transcriptional regulation, cell adhesion, metabolism, immune response, and other functions. These data provide a basis for further investigations into the molecular bases underlying the physiological abnormalities associated with VDR- and vitamin D-deficiency.

Authors+Show Affiliations

Functional Genomics Facility, University of Chicago, Chicago, Illinois 60637, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12858337

Citation

Li, Xinmin, et al. "Altered Gene Expression Profile in the Kidney of Vitamin D Receptor Knockout Mice." Journal of Cellular Biochemistry, vol. 89, no. 4, 2003, pp. 709-19.
Li X, Zheng W, Li YC. Altered gene expression profile in the kidney of vitamin D receptor knockout mice. J Cell Biochem. 2003;89(4):709-19.
Li, X., Zheng, W., & Li, Y. C. (2003). Altered gene expression profile in the kidney of vitamin D receptor knockout mice. Journal of Cellular Biochemistry, 89(4), pp. 709-19.
Li X, Zheng W, Li YC. Altered Gene Expression Profile in the Kidney of Vitamin D Receptor Knockout Mice. J Cell Biochem. 2003 Jul 1;89(4):709-19. PubMed PMID: 12858337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered gene expression profile in the kidney of vitamin D receptor knockout mice. AU - Li,Xinmin, AU - Zheng,Wei, AU - Li,Yan Chun, PY - 2003/7/15/pubmed PY - 2003/12/3/medline PY - 2003/7/15/entrez SP - 709 EP - 19 JF - Journal of cellular biochemistry JO - J. Cell. Biochem. VL - 89 IS - 4 N2 - The kidney is a primary target organ of the vitamin D endocrine system, and both vitamin D-deficiency and vitamin D receptor (VDR) ablation lead to impaired renal functions. As an initial step to understand the molecular basis underlying the renal dysfunctions resulted from VDR inactivation, we used DNA microarray technology to search for changes in the gene expression profile in the kidney of VDR knockout mice. Three independent DNA microarray experiments were performed using Affymetrix GeneChips, which included two replicate comparisons between VDR null and wild-type littermates, and a third comparison between 1,25-dihydroxyvitamin D(3)-treated and vehicle-treated wild-type mice. Based on the assumption that VDR inactivation and vitamin D stimulation cause opposite changes in the expression of vitamin D target genes, we identified 95 genes that displayed the same changes in the two VDR-null/wild-type comparisons but an opposite change in the third assay, of which 28 genes were up-regulated and 67 were down-regulated in VDR null mice. These genes can be divided into several functional categories involved in vitamin D and steroid metabolism, calcium metabolism and signaling, volume and electrolyte homeostasis, signal transduction, transcriptional regulation, cell adhesion, metabolism, immune response, and other functions. These data provide a basis for further investigations into the molecular bases underlying the physiological abnormalities associated with VDR- and vitamin D-deficiency. SN - 0730-2312 UR - https://www.unboundmedicine.com/medline/citation/12858337/Altered_gene_expression_profile_in_the_kidney_of_vitamin_D_receptor_knockout_mice_ L2 - https://doi.org/10.1002/jcb.10547 DB - PRIME DP - Unbound Medicine ER -