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Relevance of B19 markers in serum samples for a diagnosis of parvovirus B19-correlated diseases.
J Med Virol. 2003 Sep; 71(1):135-9.JM

Abstract

In order to evaluate the optimal and essential diagnostic test(s) for a correct diagnosis of B19 diseases, 344 consecutive serum samples were tested from 344 patients with clinical suspicion of B19 infection during an epidemic period (early Spring-Autumn 2000). Sera were tested for B19 DNA by a standardized competitive polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA) and dot-blot hybridization and for specific IgM and IgG by ELISA. Of 344 patients examined, 125 were positive for markers of B19-associated disease: 49 had both B19 DNA and IgM, 50 had B19 DNA without IgM, and 26 had IgM without B19 DNA. After examination of the different patterns of B19 markers as diagnostic tools for B19 infection, IgM determination detected only 60% of B19-documented infections. IgM tests were nevertheless fundamental, as they were the unique diagnostic marker in 20.8% of documented infections (26 of 125 patients), in the diagnosis of recent, but still symptomatic infections when B19 DNA was no longer detectable. The determination of B19 DNA with PCR permitted detection of 79.2% of infections and therefore represented an essential test. PCR was fundamental for the diagnosis of B19 disease, as the unique diagnostic marker in 32% of documented infections (50 of 125 patients), both in acute infections at the onset of symptoms before the appearance of immunological response, and during the course of persistent B19 infections in which IgM had cleared. The contemporaneous determination of B19 DNA by PCR and specific IgM appears to be the most appropriate diagnostic protocol for the correct laboratory diagnosis of B19 infection.

Authors+Show Affiliations

Division of Microbiology, Department of Clinical and Experimental Medicine, University of Bologna, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12858419

Citation

Gallinella, G, et al. "Relevance of B19 Markers in Serum Samples for a Diagnosis of Parvovirus B19-correlated Diseases." Journal of Medical Virology, vol. 71, no. 1, 2003, pp. 135-9.
Gallinella G, Zuffi E, Gentilomi G, et al. Relevance of B19 markers in serum samples for a diagnosis of parvovirus B19-correlated diseases. J Med Virol. 2003;71(1):135-9.
Gallinella, G., Zuffi, E., Gentilomi, G., Manaresi, E., Venturoli, S., Bonvicini, F., Cricca, M., Zerbini, M., & Musiani, M. (2003). Relevance of B19 markers in serum samples for a diagnosis of parvovirus B19-correlated diseases. Journal of Medical Virology, 71(1), 135-9.
Gallinella G, et al. Relevance of B19 Markers in Serum Samples for a Diagnosis of Parvovirus B19-correlated Diseases. J Med Virol. 2003;71(1):135-9. PubMed PMID: 12858419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relevance of B19 markers in serum samples for a diagnosis of parvovirus B19-correlated diseases. AU - Gallinella,G, AU - Zuffi,E, AU - Gentilomi,G, AU - Manaresi,E, AU - Venturoli,S, AU - Bonvicini,F, AU - Cricca,M, AU - Zerbini,M, AU - Musiani,M, PY - 2003/7/15/pubmed PY - 2003/10/8/medline PY - 2003/7/15/entrez SP - 135 EP - 9 JF - Journal of medical virology JO - J Med Virol VL - 71 IS - 1 N2 - In order to evaluate the optimal and essential diagnostic test(s) for a correct diagnosis of B19 diseases, 344 consecutive serum samples were tested from 344 patients with clinical suspicion of B19 infection during an epidemic period (early Spring-Autumn 2000). Sera were tested for B19 DNA by a standardized competitive polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA) and dot-blot hybridization and for specific IgM and IgG by ELISA. Of 344 patients examined, 125 were positive for markers of B19-associated disease: 49 had both B19 DNA and IgM, 50 had B19 DNA without IgM, and 26 had IgM without B19 DNA. After examination of the different patterns of B19 markers as diagnostic tools for B19 infection, IgM determination detected only 60% of B19-documented infections. IgM tests were nevertheless fundamental, as they were the unique diagnostic marker in 20.8% of documented infections (26 of 125 patients), in the diagnosis of recent, but still symptomatic infections when B19 DNA was no longer detectable. The determination of B19 DNA with PCR permitted detection of 79.2% of infections and therefore represented an essential test. PCR was fundamental for the diagnosis of B19 disease, as the unique diagnostic marker in 32% of documented infections (50 of 125 patients), both in acute infections at the onset of symptoms before the appearance of immunological response, and during the course of persistent B19 infections in which IgM had cleared. The contemporaneous determination of B19 DNA by PCR and specific IgM appears to be the most appropriate diagnostic protocol for the correct laboratory diagnosis of B19 infection. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/12858419/Relevance_of_B19_markers_in_serum_samples_for_a_diagnosis_of_parvovirus_B19_correlated_diseases_ L2 - https://doi.org/10.1002/jmv.10452 DB - PRIME DP - Unbound Medicine ER -