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Confirmation of amphetamine, methamphetamine, MDA and MDMA in urine samples using disk solid-phase extraction and gas chromatography-mass spectrometry after immunoassay screening.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 25; 792(2):241-7.JC

Abstract

A method using mixed phase disk solid-phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS) was developed for confirmation of amphetamine (AMP), methamphetamine (MET), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA) in urine samples after immunoassay screening. Disk SPE provided hydrophobic (C(18)) and strong cation-exchange (SCX) interactions. The analytes were retained on SCX functional groups in the disk and eluted with ammoniated ethyl acetate after washed with methanol. Confirmation and quantitation was exercised by selected ion monitoring using nikethamide as chromatographic standard. Recoveries of the amphetamines were between 73.0 and 104.6% with RSDs in range of 2.1-6.4% (n=3). The limits of detection were 2 ng/ml for AMP, MET and MDMA, and 4 ng/ml for MDA. Five real urine samples were tested with the method after immunoassay screening, and the results were comparable to those of traditional liquid-liquid extraction (LLE). The method was solvent-saved, simple, rapid and reliable, and the extract was cleaner than that of LLE.

Authors+Show Affiliations

Forensic Sciences Institute, Fujian Provincial Department of Public Security, Fuzhou 350003, China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12860031

Citation

Huang, Zengping, and Shaoyu Zhang. "Confirmation of Amphetamine, Methamphetamine, MDA and MDMA in Urine Samples Using Disk Solid-phase Extraction and Gas Chromatography-mass Spectrometry After Immunoassay Screening." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 792, no. 2, 2003, pp. 241-7.
Huang Z, Zhang S. Confirmation of amphetamine, methamphetamine, MDA and MDMA in urine samples using disk solid-phase extraction and gas chromatography-mass spectrometry after immunoassay screening. J Chromatogr B Analyt Technol Biomed Life Sci. 2003;792(2):241-7.
Huang, Z., & Zhang, S. (2003). Confirmation of amphetamine, methamphetamine, MDA and MDMA in urine samples using disk solid-phase extraction and gas chromatography-mass spectrometry after immunoassay screening. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 792(2), 241-7.
Huang Z, Zhang S. Confirmation of Amphetamine, Methamphetamine, MDA and MDMA in Urine Samples Using Disk Solid-phase Extraction and Gas Chromatography-mass Spectrometry After Immunoassay Screening. J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 25;792(2):241-7. PubMed PMID: 12860031.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Confirmation of amphetamine, methamphetamine, MDA and MDMA in urine samples using disk solid-phase extraction and gas chromatography-mass spectrometry after immunoassay screening. AU - Huang,Zengping, AU - Zhang,Shaoyu, PY - 2003/7/16/pubmed PY - 2004/3/25/medline PY - 2003/7/16/entrez SP - 241 EP - 7 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 792 IS - 2 N2 - A method using mixed phase disk solid-phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS) was developed for confirmation of amphetamine (AMP), methamphetamine (MET), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA) in urine samples after immunoassay screening. Disk SPE provided hydrophobic (C(18)) and strong cation-exchange (SCX) interactions. The analytes were retained on SCX functional groups in the disk and eluted with ammoniated ethyl acetate after washed with methanol. Confirmation and quantitation was exercised by selected ion monitoring using nikethamide as chromatographic standard. Recoveries of the amphetamines were between 73.0 and 104.6% with RSDs in range of 2.1-6.4% (n=3). The limits of detection were 2 ng/ml for AMP, MET and MDMA, and 4 ng/ml for MDA. Five real urine samples were tested with the method after immunoassay screening, and the results were comparable to those of traditional liquid-liquid extraction (LLE). The method was solvent-saved, simple, rapid and reliable, and the extract was cleaner than that of LLE. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/12860031/Confirmation_of_amphetamine_methamphetamine_MDA_and_MDMA_in_urine_samples_using_disk_solid_phase_extraction_and_gas_chromatography_mass_spectrometry_after_immunoassay_screening_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570023203002691 DB - PRIME DP - Unbound Medicine ER -