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Determination of biogenic amines by 3-(2-furoyl)quinoline-2-carboxaldehyde and capillary electrophoresis with laser-induced fluorescence detection.
J Chromatogr A. 2003 May 23; 998(1-2):213-9.JC

Abstract

Micellar electrokinetic capillary chromatography with laser-induced fluorescence detection was applied to the determination of biogenic amines including putrescine, histamine, cadaverine, tyramine, tryptamine, 2-phenylethylamine, spermine, and spermidine. A fluorogenic derivatization reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde, was successfully used to fluorescently label these biogenic amines. Different variables that affect derivatization (derivatization reagent concentration, reaction time and temperature) and separation (buffer concentration, addition of organic modifiers and sodium deoxycholate concentration) were studied. The linearities within concentration ranges of up to two orders of magnitudes were achieved for those species with correlation coefficients from 0.9967 to 0.9992. The detection limits (signal to noise = 3) of biogenic amines can reach 5 x 10(-10) mol l(-1), which are equivalent to or better than the detection limits obtained by other analytical methods of biogenic amines. As a preliminary application, this method has been successfully employed to determine biogenic amines in the extract of tobacco leaf.

Authors+Show Affiliations

Liu X
Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Yang LX
No affiliation info available
Lu YT
No affiliation info available

MeSH

Biogenic AminesCalibrationElectrophoresis, CapillaryLasersReproducibility of ResultsSensitivity and SpecificitySpectrometry, Fluorescence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12862385

Citation

Liu, Xin, et al. "Determination of Biogenic Amines By 3-(2-furoyl)quinoline-2-carboxaldehyde and Capillary Electrophoresis With Laser-induced Fluorescence Detection." Journal of Chromatography. A, vol. 998, no. 1-2, 2003, pp. 213-9.
Liu X, Yang LX, Lu YT. Determination of biogenic amines by 3-(2-furoyl)quinoline-2-carboxaldehyde and capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2003;998(1-2):213-9.
Liu, X., Yang, L. X., & Lu, Y. T. (2003). Determination of biogenic amines by 3-(2-furoyl)quinoline-2-carboxaldehyde and capillary electrophoresis with laser-induced fluorescence detection. Journal of Chromatography. A, 998(1-2), 213-9.
Liu X, Yang LX, Lu YT. Determination of Biogenic Amines By 3-(2-furoyl)quinoline-2-carboxaldehyde and Capillary Electrophoresis With Laser-induced Fluorescence Detection. J Chromatogr A. 2003 May 23;998(1-2):213-9. PubMed PMID: 12862385.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of biogenic amines by 3-(2-furoyl)quinoline-2-carboxaldehyde and capillary electrophoresis with laser-induced fluorescence detection. AU - Liu,Xin, AU - Yang,Li-Xiang, AU - Lu,Ying-Tang, PY - 2003/7/17/pubmed PY - 2004/3/3/medline PY - 2003/7/17/entrez SP - 213 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 998 IS - 1-2 N2 - Micellar electrokinetic capillary chromatography with laser-induced fluorescence detection was applied to the determination of biogenic amines including putrescine, histamine, cadaverine, tyramine, tryptamine, 2-phenylethylamine, spermine, and spermidine. A fluorogenic derivatization reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde, was successfully used to fluorescently label these biogenic amines. Different variables that affect derivatization (derivatization reagent concentration, reaction time and temperature) and separation (buffer concentration, addition of organic modifiers and sodium deoxycholate concentration) were studied. The linearities within concentration ranges of up to two orders of magnitudes were achieved for those species with correlation coefficients from 0.9967 to 0.9992. The detection limits (signal to noise = 3) of biogenic amines can reach 5 x 10(-10) mol l(-1), which are equivalent to or better than the detection limits obtained by other analytical methods of biogenic amines. As a preliminary application, this method has been successfully employed to determine biogenic amines in the extract of tobacco leaf. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/12862385/Determination_of_biogenic_amines_by_3__2_furoyl_quinoline_2_carboxaldehyde_and_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -