Tags

Type your tag names separated by a space and hit enter

[Antibody response of patients with severe acute respiratory syndrome (SARS) to nucleocapsid antigen of SARS-associated coronavirus].
Di Yi Jun Yi Da Xue Xue Bao. 2003 Jul; 23(7):637-9.DY

Abstract

OBJECTIVE

To assess serum antibody responses of patients with severe acute respiratory syndrome (SARS) to nucleocapsid (N) antigen of SARS-associated coronavirus.

METHODS

The serum levels of IgM and IgG antibodies to N antigen were measured in 200 healthy blood donors and 13 SARS patients at different time points of acute and convalescent phases using indirect enzyme-linked immunosorbent assay (ELISA) with N fusion proteins of SARS-associated coronaviruses.

RESULTS

The IgM positive critical value of 0.233 and IgG of 0.239 were selected as the threshold value for positive results that equals the product of 2.1 and the mean IgM and IgG levels of 200 healthy blood donors. In 13 patients with SARS, the antibody responses to N antigen were not detectable in the first week after the onset of symptoms. The IgM and IgG seroprotection rates were 83.3% and 66.7% respectively in the second week, both reaching 100% at the third week. IgM seroprotection rates was 61.5% in the second month, and 38.5% at third month. The IgG peaked one month after the onset and remained at high levels in the following 2 months.

CONCLUSION

The antibody responses suggest that N protein of SARS is immunodominant and plays an important role in viral pathogenesis. This ELISA-based test for detecting anti- N antigen may be of significant value for SARS diagnosis.

Authors+Show Affiliations

Che XY
Central Laboratory, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China. linche@pub.guangzhou.gd.cn
Hao W
No affiliation info available
Qiu LW
No affiliation info available
Pan YX
No affiliation info available
Liao ZY
No affiliation info available
Xu H
No affiliation info available
Chen JJ
No affiliation info available
Hou JL
No affiliation info available
Woo PC
No affiliation info available
Lau SK
No affiliation info available
Kwok YY
No affiliation info available
Huang Z
No affiliation info available

MeSH

Antibodies, ViralAntigens, ViralHumansImmunoglobulin GImmunoglobulin MNucleocapsid ProteinsSevere acute respiratory syndrome-related coronavirusSerologic TestsSevere Acute Respiratory Syndrome

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12865207

Citation

Che, Xiao-yan, et al. "[Antibody Response of Patients With Severe Acute Respiratory Syndrome (SARS) to Nucleocapsid Antigen of SARS-associated Coronavirus]." Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, vol. 23, no. 7, 2003, pp. 637-9.
Che XY, Hao W, Qiu LW, et al. [Antibody response of patients with severe acute respiratory syndrome (SARS) to nucleocapsid antigen of SARS-associated coronavirus]. Di Yi Jun Yi Da Xue Xue Bao. 2003;23(7):637-9.
Che, X. Y., Hao, W., Qiu, L. W., Pan, Y. X., Liao, Z. Y., Xu, H., Chen, J. J., Hou, J. L., Woo, P. C., Lau, S. K., Kwok, Y. Y., & Huang, Z. (2003). [Antibody response of patients with severe acute respiratory syndrome (SARS) to nucleocapsid antigen of SARS-associated coronavirus]. Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, 23(7), 637-9.
Che XY, et al. [Antibody Response of Patients With Severe Acute Respiratory Syndrome (SARS) to Nucleocapsid Antigen of SARS-associated Coronavirus]. Di Yi Jun Yi Da Xue Xue Bao. 2003;23(7):637-9. PubMed PMID: 12865207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Antibody response of patients with severe acute respiratory syndrome (SARS) to nucleocapsid antigen of SARS-associated coronavirus]. AU - Che,Xiao-yan, AU - Hao,Wei, AU - Qiu,Li-wen, AU - Pan,Yu-xian, AU - Liao,Zhi-yong, AU - Xu,Hua, AU - Chen,Jin-jun, AU - Hou,Jin-lin, AU - Woo,Patrick C Y, AU - Lau,Susanna K P, AU - Kwok,Yung Yuen, AU - Huang,Zhen, PY - 2003/7/17/pubmed PY - 2004/1/30/medline PY - 2003/7/17/entrez SP - 637 EP - 9 JF - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA JO - Di Yi Jun Yi Da Xue Xue Bao VL - 23 IS - 7 N2 - OBJECTIVE: To assess serum antibody responses of patients with severe acute respiratory syndrome (SARS) to nucleocapsid (N) antigen of SARS-associated coronavirus. METHODS: The serum levels of IgM and IgG antibodies to N antigen were measured in 200 healthy blood donors and 13 SARS patients at different time points of acute and convalescent phases using indirect enzyme-linked immunosorbent assay (ELISA) with N fusion proteins of SARS-associated coronaviruses. RESULTS: The IgM positive critical value of 0.233 and IgG of 0.239 were selected as the threshold value for positive results that equals the product of 2.1 and the mean IgM and IgG levels of 200 healthy blood donors. In 13 patients with SARS, the antibody responses to N antigen were not detectable in the first week after the onset of symptoms. The IgM and IgG seroprotection rates were 83.3% and 66.7% respectively in the second week, both reaching 100% at the third week. IgM seroprotection rates was 61.5% in the second month, and 38.5% at third month. The IgG peaked one month after the onset and remained at high levels in the following 2 months. CONCLUSION: The antibody responses suggest that N protein of SARS is immunodominant and plays an important role in viral pathogenesis. This ELISA-based test for detecting anti- N antigen may be of significant value for SARS diagnosis. SN - 1000-2588 UR - https://www.unboundmedicine.com/medline/citation/12865207/[Antibody_response_of_patients_with_severe_acute_respiratory_syndrome__SARS__to_nucleocapsid_antigen_of_SARS_associated_coronavirus]_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓13]

Related Citations

More