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In situ localization of cell wall alpha-1,3-glucan in the fission yeast Schizosaccharomyces pombe.
J Electron Microsc (Tokyo). 2003; 52(2):237-42.JE

Abstract

The yeast cell walls of the budding yeast Saccharomyces cerevisiae are well studied and the results show the existence of a framework composed of beta-1,3-glucan. It is reported that the cell wall of the fission yeast Schizosaccharomyces pombe has different components and our analysis by 13C-nuclear magnetic resonance (NMR) spectroscopy also showed there is alpha-1,3-glucan in its cell wall. To refine our understanding of the architecture of the yeast cell wall, we re-examined the cell wall glucans of S. pombe by NMR spectroscopy and prepared antibody against alpha-1,3-glucan, which is a characteristic component of this yeast. By the competitive enzyme-linked immunosorbent assay (ELISA) system, specificity of the antibody was restricted to alpha-1,3-glucan, which did not take a highly ordered structure. We analysed the localization of the cell wall glucans by immunoelectron microscopy. Transmission electron microscope (TEM) images showed that most of the alpha-1,3-glucan was along the cell membrane and appeared to enclose the cytoplasm, supporting previous reports that this glucan is synthesized on the cell membrane.

Authors

Sugawara T
No affiliation info available
Sato M
No affiliation info available
Takagi T
No affiliation info available
Kamasaki T
No affiliation info available
Ohno N
No affiliation info available
Osumi M
No affiliation info available

MeSH

Antibodies, MonoclonalCell WallFreezingGlucansMagnetic Resonance SpectroscopyMicroscopy, ElectronPressureSchizosaccharomyces

Pub Type(s)

Letter

Language

eng

PubMed ID

12868594

Citation

Sugawara, Tomoko, et al. "In Situ Localization of Cell Wall Alpha-1,3-glucan in the Fission Yeast Schizosaccharomyces Pombe." Journal of Electron Microscopy, vol. 52, no. 2, 2003, pp. 237-42.
Sugawara T, Sato M, Takagi T, et al. In situ localization of cell wall alpha-1,3-glucan in the fission yeast Schizosaccharomyces pombe. J Electron Microsc (Tokyo). 2003;52(2):237-42.
Sugawara, T., Sato, M., Takagi, T., Kamasaki, T., Ohno, N., & Osumi, M. (2003). In situ localization of cell wall alpha-1,3-glucan in the fission yeast Schizosaccharomyces pombe. Journal of Electron Microscopy, 52(2), 237-42.
Sugawara T, et al. In Situ Localization of Cell Wall Alpha-1,3-glucan in the Fission Yeast Schizosaccharomyces Pombe. J Electron Microsc (Tokyo). 2003;52(2):237-42. PubMed PMID: 12868594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ localization of cell wall alpha-1,3-glucan in the fission yeast Schizosaccharomyces pombe. AU - Sugawara,Tomoko, AU - Sato,Mamiko, AU - Takagi,Tomoko, AU - Kamasaki,Tomoko, AU - Ohno,Naohito, AU - Osumi,Masako, PY - 2003/7/19/pubmed PY - 2003/9/11/medline PY - 2003/7/19/entrez SP - 237 EP - 42 JF - Journal of electron microscopy JO - J Electron Microsc (Tokyo) VL - 52 IS - 2 N2 - The yeast cell walls of the budding yeast Saccharomyces cerevisiae are well studied and the results show the existence of a framework composed of beta-1,3-glucan. It is reported that the cell wall of the fission yeast Schizosaccharomyces pombe has different components and our analysis by 13C-nuclear magnetic resonance (NMR) spectroscopy also showed there is alpha-1,3-glucan in its cell wall. To refine our understanding of the architecture of the yeast cell wall, we re-examined the cell wall glucans of S. pombe by NMR spectroscopy and prepared antibody against alpha-1,3-glucan, which is a characteristic component of this yeast. By the competitive enzyme-linked immunosorbent assay (ELISA) system, specificity of the antibody was restricted to alpha-1,3-glucan, which did not take a highly ordered structure. We analysed the localization of the cell wall glucans by immunoelectron microscopy. Transmission electron microscope (TEM) images showed that most of the alpha-1,3-glucan was along the cell membrane and appeared to enclose the cytoplasm, supporting previous reports that this glucan is synthesized on the cell membrane. SN - 0022-0744 UR - https://www.unboundmedicine.com/medline/citation/12868594/In_situ_localization_of_cell_wall_alpha_13_glucan_in_the_fission_yeast_Schizosaccharomyces_pombe_ DB - PRIME DP - Unbound Medicine ER -