Tags

Type your tag names separated by a space and hit enter

The immune response modifier and Toll-like receptor 7 agonist S-27609 selectively induces IL-12 and TNF-alpha production in CD11c+CD11b+CD8- dendritic cells.
J Immunol. 2003 Aug 01; 171(3):1156-63.JI

Abstract

IL-12 and TNF-alpha production by dendritic cells (DCs) is a critical step in the initiation of local inflammation and adaptive immune responses. We show in this study that a small molecule immune response modifier that is a Toll-like receptor 7 (TLR7) agonist induces IL-12 and TNF-alpha production from murine CD11c(+)CD11b(+)CD8(-) DCs, a subset not previously known for this activity. Stimulation of these DCs through TLR7 in vivo induces significant cytokine production even 12 h after initial stimulation, as well as migration of the DC into T cell zones of the lymphoid tissue. In contrast, stimulation through TLR4 and TLR9 induced IL-12 production predominantly from CD8(+) DCs, consistent with previously published data. All TLR stimuli induced the increase in surface expression of the activation markers B7-1, B7-2, and class II in both CD8(+) and CD8(-) DCs, demonstrating that CD8(+) DCs do respond to TLR7-mediated stimuli. To date this is the only known stimuli to induce preferential cytokine production from CD8(-) DCs. Given the efficacy of TLR7 agonists as antiviral agents, the data collectively indicate that stimulation of CD8(-) DCs through TLR7 most likely plays a role in the generation of antiviral immune responses.

Authors+Show Affiliations

Department of Pharmacology, 3 M Pharmaceuticals, St Paul, MN 55144, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12874201

Citation

Doxsee, Christie L., et al. "The Immune Response Modifier and Toll-like Receptor 7 Agonist S-27609 Selectively Induces IL-12 and TNF-alpha Production in CD11c+CD11b+CD8- Dendritic Cells." Journal of Immunology (Baltimore, Md. : 1950), vol. 171, no. 3, 2003, pp. 1156-63.
Doxsee CL, Riter TR, Reiter MJ, et al. The immune response modifier and Toll-like receptor 7 agonist S-27609 selectively induces IL-12 and TNF-alpha production in CD11c+CD11b+CD8- dendritic cells. J Immunol. 2003;171(3):1156-63.
Doxsee, C. L., Riter, T. R., Reiter, M. J., Gibson, S. J., Vasilakos, J. P., & Kedl, R. M. (2003). The immune response modifier and Toll-like receptor 7 agonist S-27609 selectively induces IL-12 and TNF-alpha production in CD11c+CD11b+CD8- dendritic cells. Journal of Immunology (Baltimore, Md. : 1950), 171(3), 1156-63.
Doxsee CL, et al. The Immune Response Modifier and Toll-like Receptor 7 Agonist S-27609 Selectively Induces IL-12 and TNF-alpha Production in CD11c+CD11b+CD8- Dendritic Cells. J Immunol. 2003 Aug 1;171(3):1156-63. PubMed PMID: 12874201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The immune response modifier and Toll-like receptor 7 agonist S-27609 selectively induces IL-12 and TNF-alpha production in CD11c+CD11b+CD8- dendritic cells. AU - Doxsee,Christie L, AU - Riter,Tony R, AU - Reiter,Michael J, AU - Gibson,Shelia J, AU - Vasilakos,John P, AU - Kedl,Ross M, PY - 2003/7/23/pubmed PY - 2003/11/8/medline PY - 2003/7/23/entrez SP - 1156 EP - 63 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 171 IS - 3 N2 - IL-12 and TNF-alpha production by dendritic cells (DCs) is a critical step in the initiation of local inflammation and adaptive immune responses. We show in this study that a small molecule immune response modifier that is a Toll-like receptor 7 (TLR7) agonist induces IL-12 and TNF-alpha production from murine CD11c(+)CD11b(+)CD8(-) DCs, a subset not previously known for this activity. Stimulation of these DCs through TLR7 in vivo induces significant cytokine production even 12 h after initial stimulation, as well as migration of the DC into T cell zones of the lymphoid tissue. In contrast, stimulation through TLR4 and TLR9 induced IL-12 production predominantly from CD8(+) DCs, consistent with previously published data. All TLR stimuli induced the increase in surface expression of the activation markers B7-1, B7-2, and class II in both CD8(+) and CD8(-) DCs, demonstrating that CD8(+) DCs do respond to TLR7-mediated stimuli. To date this is the only known stimuli to induce preferential cytokine production from CD8(-) DCs. Given the efficacy of TLR7 agonists as antiviral agents, the data collectively indicate that stimulation of CD8(-) DCs through TLR7 most likely plays a role in the generation of antiviral immune responses. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/12874201/The_immune_response_modifier_and_Toll_like_receptor_7_agonist_S_27609_selectively_induces_IL_12_and_TNF_alpha_production_in_CD11c+CD11b+CD8__dendritic_cells_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=12874201 DB - PRIME DP - Unbound Medicine ER -