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Effect of inactivation of the Arg- and/or Lys-gingipain gene on selected virulence and physiological properties of Porphyromonas gingivalis.
Infect Immun. 2003 Aug; 71(8):4742-8.II

Abstract

Proteolytic enzymes produced by Porphyromonas gingivalis are thought to play critical roles in the pathogenesis of periodontitis. The aim of this study was to investigate the effect of gingipain cysteine proteinase gene inactivation on selected pathological and physiological functions of P. gingivalis. Our results showed that Arg- and Lys-gingipain activities are critical components for the efficient growth of P. gingivalis in human serum. However, when the serum was supplemented with peptides provided as pancreatic casein hydrolysate, the gingipains did not appear to be essential for growth. The effect of gingipain gene inactivation on the susceptibility of P. gingivalis to serum bactericidal activity was investigated using standardized human serum. The wild-type strain, P. gingivalis ATCC 33277, was largely unaffected by the bactericidal activity of human serum complement. On the other hand, mutants lacking Arg-gingipain A, Arg-gingipain B, or Lys-gingipain activity were susceptible to complement. Since gingipains are mostly located on the outer membrane of P. gingivalis, inactivation of the genes for these enzymes may modify cell surface properties. We showed that gingipain-deficient mutants differed in their capacities to assimilate radiolabeled amino acids, cause hemolysis, express adhesins, hemagglutinate, and form biofilms. Lastly, the gingipains, more specifically Arg-gingipains, were responsible for causing major cell damage to human gingival fibroblasts. In conclusion, our study indicated that, in addition to being critical in the pathogenic process, gingipains may play a variety of physiological roles in P. gingivalis, including controlling the expression and/or processing of virulence factors. Mutations in gingipain genes thus give rise to pleiotropic effects.

Authors+Show Affiliations

Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada. Daniel.Grenier@greb.ulaval.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12874356

Citation

Grenier, Daniel, et al. "Effect of Inactivation of the Arg- And/or Lys-gingipain Gene On Selected Virulence and Physiological Properties of Porphyromonas Gingivalis." Infection and Immunity, vol. 71, no. 8, 2003, pp. 4742-8.
Grenier D, Roy S, Chandad F, et al. Effect of inactivation of the Arg- and/or Lys-gingipain gene on selected virulence and physiological properties of Porphyromonas gingivalis. Infect Immun. 2003;71(8):4742-8.
Grenier, D., Roy, S., Chandad, F., Plamondon, P., Yoshioka, M., Nakayama, K., & Mayrand, D. (2003). Effect of inactivation of the Arg- and/or Lys-gingipain gene on selected virulence and physiological properties of Porphyromonas gingivalis. Infection and Immunity, 71(8), 4742-8.
Grenier D, et al. Effect of Inactivation of the Arg- And/or Lys-gingipain Gene On Selected Virulence and Physiological Properties of Porphyromonas Gingivalis. Infect Immun. 2003;71(8):4742-8. PubMed PMID: 12874356.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of inactivation of the Arg- and/or Lys-gingipain gene on selected virulence and physiological properties of Porphyromonas gingivalis. AU - Grenier,Daniel, AU - Roy,Sophie, AU - Chandad,Fatiha, AU - Plamondon,Pascale, AU - Yoshioka,Masami, AU - Nakayama,Koji, AU - Mayrand,Denis, PY - 2003/7/23/pubmed PY - 2003/8/27/medline PY - 2003/7/23/entrez SP - 4742 EP - 8 JF - Infection and immunity JO - Infect Immun VL - 71 IS - 8 N2 - Proteolytic enzymes produced by Porphyromonas gingivalis are thought to play critical roles in the pathogenesis of periodontitis. The aim of this study was to investigate the effect of gingipain cysteine proteinase gene inactivation on selected pathological and physiological functions of P. gingivalis. Our results showed that Arg- and Lys-gingipain activities are critical components for the efficient growth of P. gingivalis in human serum. However, when the serum was supplemented with peptides provided as pancreatic casein hydrolysate, the gingipains did not appear to be essential for growth. The effect of gingipain gene inactivation on the susceptibility of P. gingivalis to serum bactericidal activity was investigated using standardized human serum. The wild-type strain, P. gingivalis ATCC 33277, was largely unaffected by the bactericidal activity of human serum complement. On the other hand, mutants lacking Arg-gingipain A, Arg-gingipain B, or Lys-gingipain activity were susceptible to complement. Since gingipains are mostly located on the outer membrane of P. gingivalis, inactivation of the genes for these enzymes may modify cell surface properties. We showed that gingipain-deficient mutants differed in their capacities to assimilate radiolabeled amino acids, cause hemolysis, express adhesins, hemagglutinate, and form biofilms. Lastly, the gingipains, more specifically Arg-gingipains, were responsible for causing major cell damage to human gingival fibroblasts. In conclusion, our study indicated that, in addition to being critical in the pathogenic process, gingipains may play a variety of physiological roles in P. gingivalis, including controlling the expression and/or processing of virulence factors. Mutations in gingipain genes thus give rise to pleiotropic effects. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/12874356/Effect_of_inactivation_of_the_Arg__and/or_Lys_gingipain_gene_on_selected_virulence_and_physiological_properties_of_Porphyromonas_gingivalis_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=12874356 DB - PRIME DP - Unbound Medicine ER -