Tags

Type your tag names separated by a space and hit enter

New haplotypes of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene among chloroquine-resistant parasite isolates.
Am J Trop Med Hyg. 2003 Apr; 68(4):398-402.AJ

Abstract

Mutations in the Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene were examined to assess their associations with chloroquine resistance in clinical samples from Armopa (Papua) and Papua New Guinea. In Papua, two of the five pfcrt haplotypes found were new: SVIET from Armopa and CVIKT from an isolate in Timika. There was also a strong association (P < 0.0001) between the pfcrt 76T allele and chloroquine resistance in 50 samples. In Papua New Guinea, mutations in the pfcrt gene were observed in 15 isolates with chloroquine minimum inhibitory concentrations (MICs) of 16-64 pmol, while the remaining six isolates, which had a wild-type pfcrt gene at codon 76, had MICs of 2-8 pmol. These observations confirm that mutations at codon 76 in the pfcrt gene are present in both in vivo and in vitro cases of chloroquine resistance, and that detection of the pfcrt 76T allele could predict potential chloroquine treatment failures.

Authors+Show Affiliations

The Walter and Eliza Hall Institute for Medical Research, Melbourne, Australia. hadya@wehi.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

12875286

Citation

Nagesha, Hadya S., et al. "New Haplotypes of the Plasmodium Falciparum Chloroquine Resistance Transporter (pfcrt) Gene Among Chloroquine-resistant Parasite Isolates." The American Journal of Tropical Medicine and Hygiene, vol. 68, no. 4, 2003, pp. 398-402.
Nagesha HS, Casey GJ, Rieckmann KH, et al. New haplotypes of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene among chloroquine-resistant parasite isolates. Am J Trop Med Hyg. 2003;68(4):398-402.
Nagesha, H. S., Casey, G. J., Rieckmann, K. H., Fryauff, D. J., Laksana, B. S., Reeder, J. C., Maguire, J. D., & Baird, J. K. (2003). New haplotypes of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene among chloroquine-resistant parasite isolates. The American Journal of Tropical Medicine and Hygiene, 68(4), 398-402.
Nagesha HS, et al. New Haplotypes of the Plasmodium Falciparum Chloroquine Resistance Transporter (pfcrt) Gene Among Chloroquine-resistant Parasite Isolates. Am J Trop Med Hyg. 2003;68(4):398-402. PubMed PMID: 12875286.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New haplotypes of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene among chloroquine-resistant parasite isolates. AU - Nagesha,Hadya S, AU - Casey,Gerard J, AU - Rieckmann,Karl H, AU - Fryauff,David J, AU - Laksana,Budi S, AU - Reeder,John C, AU - Maguire,Jason D, AU - Baird,J Kevin, PY - 2003/7/24/pubmed PY - 2003/8/2/medline PY - 2003/7/24/entrez SP - 398 EP - 402 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 68 IS - 4 N2 - Mutations in the Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene were examined to assess their associations with chloroquine resistance in clinical samples from Armopa (Papua) and Papua New Guinea. In Papua, two of the five pfcrt haplotypes found were new: SVIET from Armopa and CVIKT from an isolate in Timika. There was also a strong association (P < 0.0001) between the pfcrt 76T allele and chloroquine resistance in 50 samples. In Papua New Guinea, mutations in the pfcrt gene were observed in 15 isolates with chloroquine minimum inhibitory concentrations (MICs) of 16-64 pmol, while the remaining six isolates, which had a wild-type pfcrt gene at codon 76, had MICs of 2-8 pmol. These observations confirm that mutations at codon 76 in the pfcrt gene are present in both in vivo and in vitro cases of chloroquine resistance, and that detection of the pfcrt 76T allele could predict potential chloroquine treatment failures. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/12875286/New_haplotypes_of_the_Plasmodium_falciparum_chloroquine_resistance_transporter__pfcrt__gene_among_chloroquine_resistant_parasite_isolates_ DB - PRIME DP - Unbound Medicine ER -