Tags

Type your tag names separated by a space and hit enter

Analysis of oxidized epigallocatechin gallate by liquid chromatography/mass spectrometry.
Rapid Commun Mass Spectrom. 2003; 17(16):1915-8.RC

Abstract

(-)-Epigallocatechin gallate (EGCG) of catechins changes from non-colored at around neutral pH to yellow at higher pH region in aqueous solution. The pH-dependent oxidation of EGCG was analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). LC/MS/MS analysis of EGCG and its related compounds, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin (EGC), successfully elucidated the structure relationship of EGCG solution involving the color change reaction at different pH conditions. The oxidation species produced at alkaline pH was detected at a different retention time from EGCG in the chromatograms of the EGCG sample. The oxidation species was found to correspond to M+14 (where M is the molecular weight of EGCG), which has two hydrogen atoms removed and addition of one oxygen atom to the gallyl moiety in the B-ring of EGCG.

Authors+Show Affiliations

Department of Applied Chemistry, Kansai University, Suita, Osaka 564-8680, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12876693

Citation

Mizooku, Yasuo, et al. "Analysis of Oxidized Epigallocatechin Gallate By Liquid Chromatography/mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 17, no. 16, 2003, pp. 1915-8.
Mizooku Y, Yoshikawa M, Tsuneyoshi T, et al. Analysis of oxidized epigallocatechin gallate by liquid chromatography/mass spectrometry. Rapid Commun Mass Spectrom. 2003;17(16):1915-8.
Mizooku, Y., Yoshikawa, M., Tsuneyoshi, T., & Arakawa, R. (2003). Analysis of oxidized epigallocatechin gallate by liquid chromatography/mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 17(16), 1915-8.
Mizooku Y, et al. Analysis of Oxidized Epigallocatechin Gallate By Liquid Chromatography/mass Spectrometry. Rapid Commun Mass Spectrom. 2003;17(16):1915-8. PubMed PMID: 12876693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of oxidized epigallocatechin gallate by liquid chromatography/mass spectrometry. AU - Mizooku,Yasuo, AU - Yoshikawa,Muneyoshi, AU - Tsuneyoshi,Toshihiro, AU - Arakawa,Ryuichi, PY - 2003/7/24/pubmed PY - 2003/9/23/medline PY - 2003/7/24/entrez SP - 1915 EP - 8 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 17 IS - 16 N2 - (-)-Epigallocatechin gallate (EGCG) of catechins changes from non-colored at around neutral pH to yellow at higher pH region in aqueous solution. The pH-dependent oxidation of EGCG was analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). LC/MS/MS analysis of EGCG and its related compounds, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin (EGC), successfully elucidated the structure relationship of EGCG solution involving the color change reaction at different pH conditions. The oxidation species produced at alkaline pH was detected at a different retention time from EGCG in the chromatograms of the EGCG sample. The oxidation species was found to correspond to M+14 (where M is the molecular weight of EGCG), which has two hydrogen atoms removed and addition of one oxygen atom to the gallyl moiety in the B-ring of EGCG. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/12876693/Analysis_of_oxidized_epigallocatechin_gallate_by_liquid_chromatography/mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.1135 DB - PRIME DP - Unbound Medicine ER -