Tags

Type your tag names separated by a space and hit enter

Enhanced sensitivity electrochemical assay of low-molecular-weight heparins using rotating polyion-sensitive membrane electrodes.
Anal Bioanal Chem 2003; 377(5):929-36AB

Abstract

Use of a novel rotating polycation-sensitive polymer membrane electrode yields sensors that can serve as simple potentiometric titration endpoint detectors for the determination of three FDA approved low-molecular-weight heparin (LMWH) anticoagulant drugs (Fragmin, Normiflo, and Lovenox). The rotating electrode configuration dramatically improves the reproducibility and increases the sensitivity for LMWH determinations by protamine titration. At a rotation speed of 3000 rpm, electrodes with optimized thin (50 microm) polymer membranes doped with dinonylnaphthalene sulfonate (DNNS) respond to low levels of protamine (<2 microg mL(-1)) with good precision (+/-1 mV, N=10), when protamine is infused continuously into a Tris-buffer solution, pH 7.4. When infusing protamine (at 5 microg min(-1)) continuously into solutions containing Fragmin, a clear endpoint is obtained, with the amount of protamine required to reach this endpoint proportional to the level of Fragmin present. A detection limit of less than 0.02 U mL(-1) Fragmin can be obtained via this new method, approximately one order of magnitude lower than that previously reported based on a non-rotating polycation electrode. Similar low detection limits can be achieved for potentiometric titrations of Normiflo and Lovenox. Such titrations can also be carried out in undiluted plasma samples containing the various LMWH species. In this case, detection of the LMWHs at clinically relevant concentrations (>0.2 U mL(-1)) can be readily achieved.

Authors+Show Affiliations

Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

12879195

Citation

Qin, Wei, et al. "Enhanced Sensitivity Electrochemical Assay of Low-molecular-weight Heparins Using Rotating Polyion-sensitive Membrane Electrodes." Analytical and Bioanalytical Chemistry, vol. 377, no. 5, 2003, pp. 929-36.
Qin W, Zhang W, Xiao KP, et al. Enhanced sensitivity electrochemical assay of low-molecular-weight heparins using rotating polyion-sensitive membrane electrodes. Anal Bioanal Chem. 2003;377(5):929-36.
Qin, W., Zhang, W., Xiao, K. P., & Meyerhoff, M. E. (2003). Enhanced sensitivity electrochemical assay of low-molecular-weight heparins using rotating polyion-sensitive membrane electrodes. Analytical and Bioanalytical Chemistry, 377(5), pp. 929-36.
Qin W, et al. Enhanced Sensitivity Electrochemical Assay of Low-molecular-weight Heparins Using Rotating Polyion-sensitive Membrane Electrodes. Anal Bioanal Chem. 2003;377(5):929-36. PubMed PMID: 12879195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced sensitivity electrochemical assay of low-molecular-weight heparins using rotating polyion-sensitive membrane electrodes. AU - Qin,Wei, AU - Zhang,Wei, AU - Xiao,Kang Ping, AU - Meyerhoff,Mark E, Y1 - 2003/07/19/ PY - 2003/04/23/received PY - 2003/05/21/revised PY - 2003/05/26/accepted PY - 2003/7/25/pubmed PY - 2004/2/5/medline PY - 2003/7/25/entrez SP - 929 EP - 36 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 377 IS - 5 N2 - Use of a novel rotating polycation-sensitive polymer membrane electrode yields sensors that can serve as simple potentiometric titration endpoint detectors for the determination of three FDA approved low-molecular-weight heparin (LMWH) anticoagulant drugs (Fragmin, Normiflo, and Lovenox). The rotating electrode configuration dramatically improves the reproducibility and increases the sensitivity for LMWH determinations by protamine titration. At a rotation speed of 3000 rpm, electrodes with optimized thin (50 microm) polymer membranes doped with dinonylnaphthalene sulfonate (DNNS) respond to low levels of protamine (<2 microg mL(-1)) with good precision (+/-1 mV, N=10), when protamine is infused continuously into a Tris-buffer solution, pH 7.4. When infusing protamine (at 5 microg min(-1)) continuously into solutions containing Fragmin, a clear endpoint is obtained, with the amount of protamine required to reach this endpoint proportional to the level of Fragmin present. A detection limit of less than 0.02 U mL(-1) Fragmin can be obtained via this new method, approximately one order of magnitude lower than that previously reported based on a non-rotating polycation electrode. Similar low detection limits can be achieved for potentiometric titrations of Normiflo and Lovenox. Such titrations can also be carried out in undiluted plasma samples containing the various LMWH species. In this case, detection of the LMWHs at clinically relevant concentrations (>0.2 U mL(-1)) can be readily achieved. SN - 1618-2642 UR - https://www.unboundmedicine.com/medline/citation/12879195/Enhanced_sensitivity_electrochemical_assay_of_low-molecular-weight_heparins_using_rotating_polyion-sensitive_membrane_electrodes L2 - https://dx.doi.org/10.1007/s00216-003-2083-y DB - PRIME DP - Unbound Medicine ER -