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[Ultrastructural characteristics of SARS associated virus in infected cells].
Zhonghua Bing Li Xue Za Zhi. 2003 Jun; 32(3):209-11.ZB

Abstract

OBJECTIVE

Electron microscopical study of infected cells to identify the pathogenic agent of SARS.

METHODS

Vero E6 cells infected with lung autopsy samples or nasopharyngeal swabs from SARS patients of Beijing and Guangzhou were inoculated. The supernatant and cultured cells exhibiting identifiable cytopathic effect (CPE) were prepared for electron microscopic study.

RESULTS

Examination of CPE cells on thin-section revealed characteristic coronavirus particles within the cisternae of endoplasmic reticulum, Golgi apparatus, vesicles and extracellular space. They were mainly spherical or oval in shape, annular or dense, about 80 nm in diameter. Negative-stain electron microscopy identified coronavirus particles in culture supernatant, 80 - 120 nm in diameter, with club-shaped surface projections. Elongated, rod-, kidney- or other irregular shaped virons with the size of 100 - 200 nm by 60 - 90 nm were also found in the cultured cells infected with the lung samples from the Guangdong patients. Infectious virons entered cells by endocytosis or membrane fusion and released through a budding process.

CONCLUSION

These data indicate a novel coronavirus as the causative agent of SARS. Most viral particles showed typical characteristics of coronavirus. The potential role of special shape viruses is expected to be further investigated.

Authors+Show Affiliations

Institute of Microbiology and Epidemiology, Beijing 100071, China. wangcuie1994@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

12882683

Citation

Wang, Cui-e, et al. "[Ultrastructural Characteristics of SARS Associated Virus in Infected Cells]." Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, vol. 32, no. 3, 2003, pp. 209-11.
Wang CE, Li YC, Wu XH, et al. [Ultrastructural characteristics of SARS associated virus in infected cells]. Zhonghua Bing Li Xue Za Zhi. 2003;32(3):209-11.
Wang, C. E., Li, Y. C., Wu, X. H., Cao, J. T., Yan, G., Li, J. F., Si, B. Y., Yu, M., Qin, E. D., & Zhu, Q. Y. (2003). [Ultrastructural characteristics of SARS associated virus in infected cells]. Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, 32(3), 209-11.
Wang CE, et al. [Ultrastructural Characteristics of SARS Associated Virus in Infected Cells]. Zhonghua Bing Li Xue Za Zhi. 2003;32(3):209-11. PubMed PMID: 12882683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Ultrastructural characteristics of SARS associated virus in infected cells]. AU - Wang,Cui-e, AU - Li,Yu-chuan, AU - Wu,Xiao-hong, AU - Cao,Jun-tian, AU - Yan,Ge, AU - Li,Jin-feng, AU - Si,Bing-yin, AU - Yu,Man, AU - Qin,E-de, AU - Zhu,Qing-yu, PY - 2003/7/29/pubmed PY - 2004/1/7/medline PY - 2003/7/29/entrez SP - 209 EP - 11 JF - Zhonghua bing li xue za zhi = Chinese journal of pathology JO - Zhonghua Bing Li Xue Za Zhi VL - 32 IS - 3 N2 - OBJECTIVE: Electron microscopical study of infected cells to identify the pathogenic agent of SARS. METHODS: Vero E6 cells infected with lung autopsy samples or nasopharyngeal swabs from SARS patients of Beijing and Guangzhou were inoculated. The supernatant and cultured cells exhibiting identifiable cytopathic effect (CPE) were prepared for electron microscopic study. RESULTS: Examination of CPE cells on thin-section revealed characteristic coronavirus particles within the cisternae of endoplasmic reticulum, Golgi apparatus, vesicles and extracellular space. They were mainly spherical or oval in shape, annular or dense, about 80 nm in diameter. Negative-stain electron microscopy identified coronavirus particles in culture supernatant, 80 - 120 nm in diameter, with club-shaped surface projections. Elongated, rod-, kidney- or other irregular shaped virons with the size of 100 - 200 nm by 60 - 90 nm were also found in the cultured cells infected with the lung samples from the Guangdong patients. Infectious virons entered cells by endocytosis or membrane fusion and released through a budding process. CONCLUSION: These data indicate a novel coronavirus as the causative agent of SARS. Most viral particles showed typical characteristics of coronavirus. The potential role of special shape viruses is expected to be further investigated. SN - 0529-5807 UR - https://www.unboundmedicine.com/medline/citation/12882683/[Ultrastructural_characteristics_of_SARS_associated_virus_in_infected_cells]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0529-5807&year=2003&vol=32&issue=3&fpage=209 DB - PRIME DP - Unbound Medicine ER -