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Subcutaneous transplantation of macroencapsulated porcine pancreatic endocrine cells normalizes hyperglycemia in diabetic mice.
Transplantation. 2003 Jul 27; 76(2):290-6.T

Abstract

BACKGROUND

The ultimate goal of islet transplantation is the unlimited availability of insulin-secreting cells to be transplanted in a simple procedure that requires no use of immunosuppressive drugs. Immunoisolation of xenogeneic pig islets for transplantation has great potential therapeutic benefits for treatment of diabetes.

METHODS

Approximately 4 x 10(6) porcine pancreatic endocrine cells (PEC) isolated from 6-month-old pigs were macroencapsulated in agarose-poly(styrene sulfonic acid) mixed gel and implanted into a prevascularized subcutaneous site in streptozotocin-induced C57BL/6 diabetic mice. Animals receiving an equal number of free porcine PEC were used as controls. After transplantation, nonfasting blood glucose, body weight, intraperitoneal glucose tolerance test, and immunohistologic evaluations were processed.

RESULTS

All 10 animals receiving the subcutaneous xenografts of the macroencapsulated porcine PEC normalized hyperglycemia within 5 days after transplantation, maintained the duration of normoglycemia for 24 to 76 days, and gradually gained weight. The subcutaneous xenografts of free porcine PEC could not reverse hyperglycemia. The recipient became hyperglycemic again when the implanted graft was retrieved at day 45 after transplantation. The glucose clearances were significantly ameliorated at day 21 and day 45 after transplantation when compared with those in diabetic mice. The immunohistochemical results revealed an inherent intact structure of the macroencapsulated porcine PEC and positive double-immunofluorescence staining for insulin and glucagon.

CONCLUSIONS

Subcutaneous transplantation of macroencapsulated porcine PEC normalized hyperglycemia in diabetic mice. Our results identified a potential for a favorable development of subcutaneous transplantation of porcine PEC as a cure for diabetes.

Authors+Show Affiliations

Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

12883181

Citation

Wang, Wenjing, et al. "Subcutaneous Transplantation of Macroencapsulated Porcine Pancreatic Endocrine Cells Normalizes Hyperglycemia in Diabetic Mice." Transplantation, vol. 76, no. 2, 2003, pp. 290-6.
Wang W, Gu Y, Hori H, et al. Subcutaneous transplantation of macroencapsulated porcine pancreatic endocrine cells normalizes hyperglycemia in diabetic mice. Transplantation. 2003;76(2):290-6.
Wang, W., Gu, Y., Hori, H., Sakurai, T., Hiura, A., Sumi, S., Tabata, Y., & Inoue, K. (2003). Subcutaneous transplantation of macroencapsulated porcine pancreatic endocrine cells normalizes hyperglycemia in diabetic mice. Transplantation, 76(2), 290-6.
Wang W, et al. Subcutaneous Transplantation of Macroencapsulated Porcine Pancreatic Endocrine Cells Normalizes Hyperglycemia in Diabetic Mice. Transplantation. 2003 Jul 27;76(2):290-6. PubMed PMID: 12883181.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subcutaneous transplantation of macroencapsulated porcine pancreatic endocrine cells normalizes hyperglycemia in diabetic mice. AU - Wang,Wenjing, AU - Gu,Yuanjun, AU - Hori,Hiroshi, AU - Sakurai,Tomonori, AU - Hiura,Akihito, AU - Sumi,Shoichiro, AU - Tabata,Yasuhiko, AU - Inoue,Kazutomo, PY - 2003/7/29/pubmed PY - 2003/9/5/medline PY - 2003/7/29/entrez SP - 290 EP - 6 JF - Transplantation JO - Transplantation VL - 76 IS - 2 N2 - BACKGROUND: The ultimate goal of islet transplantation is the unlimited availability of insulin-secreting cells to be transplanted in a simple procedure that requires no use of immunosuppressive drugs. Immunoisolation of xenogeneic pig islets for transplantation has great potential therapeutic benefits for treatment of diabetes. METHODS: Approximately 4 x 10(6) porcine pancreatic endocrine cells (PEC) isolated from 6-month-old pigs were macroencapsulated in agarose-poly(styrene sulfonic acid) mixed gel and implanted into a prevascularized subcutaneous site in streptozotocin-induced C57BL/6 diabetic mice. Animals receiving an equal number of free porcine PEC were used as controls. After transplantation, nonfasting blood glucose, body weight, intraperitoneal glucose tolerance test, and immunohistologic evaluations were processed. RESULTS: All 10 animals receiving the subcutaneous xenografts of the macroencapsulated porcine PEC normalized hyperglycemia within 5 days after transplantation, maintained the duration of normoglycemia for 24 to 76 days, and gradually gained weight. The subcutaneous xenografts of free porcine PEC could not reverse hyperglycemia. The recipient became hyperglycemic again when the implanted graft was retrieved at day 45 after transplantation. The glucose clearances were significantly ameliorated at day 21 and day 45 after transplantation when compared with those in diabetic mice. The immunohistochemical results revealed an inherent intact structure of the macroencapsulated porcine PEC and positive double-immunofluorescence staining for insulin and glucagon. CONCLUSIONS: Subcutaneous transplantation of macroencapsulated porcine PEC normalized hyperglycemia in diabetic mice. Our results identified a potential for a favorable development of subcutaneous transplantation of porcine PEC as a cure for diabetes. SN - 0041-1337 UR - https://www.unboundmedicine.com/medline/citation/12883181/Subcutaneous_transplantation_of_macroencapsulated_porcine_pancreatic_endocrine_cells_normalizes_hyperglycemia_in_diabetic_mice_ L2 - https://doi.org/10.1097/01.TP.0000073613.25658.4D DB - PRIME DP - Unbound Medicine ER -