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Roles of tert-butyl formate, tert-butyl alcohol and acetone in the regulation of methyl tert-butyl ether degradation by Mycobacterium austroafricanum IFP 2012.
Appl Microbiol Biotechnol. 2003 Aug; 62(2-3):256-62.AM

Abstract

Mycobacterium austroafricanum IFP 2012 is a Gram-positive strain able to grow on methyl tert-butyl ether (MTBE) as a sole carbon and energy source. The effect of two downstream metabolites of MTBE, tert-butyl formate (TBF) and tert-butyl alcohol (TBA) on MTBE degradation was investigated using resting cells. The addition of low concentrations of TBF decreased the MTBE degradation rate by about 30%. In contrast, the addition of TBA did not have a significant effect on MTBE degradation rate, even at high concentrations; and it was also shown that TBA degradation occurred only once MTBE was exhausted. At neutral pH, TBF hydrolysis involved mainly an esterase-type activity regulated by the presence of TBA. The TBF degradation rate was about four times lower than the MTBE degradation rate. Furthermore, acetone was identified as an intermediate during TBA degradation. An acetone mono-oxygenase activity, inhibited by methimazole but not by acetylene, was suggested. It was different from the MTBE/TBA mono-oxygenase and, thus, acetone did not appear to compete with MTBE and TBA for the same enzyme. These new results show that the metabolic regulation of the early steps of MTBE degradation by M. austroafricanum IFP 2012 is complex, involving inhibition and competition phenomena.

Authors+Show Affiliations

Département de Microbiologie, Institut Français du Pétrole, 1 & 4 avenue de Bois-Préau, 92852, Rueil-Malmaison Cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

12883872

Citation

François, A, et al. "Roles of Tert-butyl Formate, Tert-butyl Alcohol and Acetone in the Regulation of Methyl Tert-butyl Ether Degradation By Mycobacterium Austroafricanum IFP 2012." Applied Microbiology and Biotechnology, vol. 62, no. 2-3, 2003, pp. 256-62.
François A, Garnier L, Mathis H, et al. Roles of tert-butyl formate, tert-butyl alcohol and acetone in the regulation of methyl tert-butyl ether degradation by Mycobacterium austroafricanum IFP 2012. Appl Microbiol Biotechnol. 2003;62(2-3):256-62.
François, A., Garnier, L., Mathis, H., Fayolle, F., & Monot, F. (2003). Roles of tert-butyl formate, tert-butyl alcohol and acetone in the regulation of methyl tert-butyl ether degradation by Mycobacterium austroafricanum IFP 2012. Applied Microbiology and Biotechnology, 62(2-3), 256-62.
François A, et al. Roles of Tert-butyl Formate, Tert-butyl Alcohol and Acetone in the Regulation of Methyl Tert-butyl Ether Degradation By Mycobacterium Austroafricanum IFP 2012. Appl Microbiol Biotechnol. 2003;62(2-3):256-62. PubMed PMID: 12883872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Roles of tert-butyl formate, tert-butyl alcohol and acetone in the regulation of methyl tert-butyl ether degradation by Mycobacterium austroafricanum IFP 2012. AU - François,A, AU - Garnier,L, AU - Mathis,H, AU - Fayolle,F, AU - Monot,F, Y1 - 2003/03/20/ PY - 2002/08/26/received PY - 2003/01/20/revised PY - 2003/01/24/accepted PY - 2003/7/29/pubmed PY - 2003/11/7/medline PY - 2003/7/29/entrez SP - 256 EP - 62 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 62 IS - 2-3 N2 - Mycobacterium austroafricanum IFP 2012 is a Gram-positive strain able to grow on methyl tert-butyl ether (MTBE) as a sole carbon and energy source. The effect of two downstream metabolites of MTBE, tert-butyl formate (TBF) and tert-butyl alcohol (TBA) on MTBE degradation was investigated using resting cells. The addition of low concentrations of TBF decreased the MTBE degradation rate by about 30%. In contrast, the addition of TBA did not have a significant effect on MTBE degradation rate, even at high concentrations; and it was also shown that TBA degradation occurred only once MTBE was exhausted. At neutral pH, TBF hydrolysis involved mainly an esterase-type activity regulated by the presence of TBA. The TBF degradation rate was about four times lower than the MTBE degradation rate. Furthermore, acetone was identified as an intermediate during TBA degradation. An acetone mono-oxygenase activity, inhibited by methimazole but not by acetylene, was suggested. It was different from the MTBE/TBA mono-oxygenase and, thus, acetone did not appear to compete with MTBE and TBA for the same enzyme. These new results show that the metabolic regulation of the early steps of MTBE degradation by M. austroafricanum IFP 2012 is complex, involving inhibition and competition phenomena. SN - 0175-7598 UR - https://www.unboundmedicine.com/medline/citation/12883872/Roles_of_tert_butyl_formate_tert_butyl_alcohol_and_acetone_in_the_regulation_of_methyl_tert_butyl_ether_degradation_by_Mycobacterium_austroafricanum_IFP_2012_ L2 - https://dx.doi.org/10.1007/s00253-003-1268-9 DB - PRIME DP - Unbound Medicine ER -